Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CXCL10/IP-10/CRG-2 Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR3. The ED₅₀ for this effect is 0.05-0.3 µg/mL.
• Source: E. coli-derived mouse CXCL10/IP-10/CRG-2 protein
  Ile22-Pro98, with an N-terminal Met
• Accession #: Q548V9
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cxcl10
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity2
• Theoretical MW: 8.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL10/IP-10/CRG-2 Protein

• C7
• chemokine (C-X-C motif) ligand 10
• CRG2
• CRG-2
• CXCL10
• gIP-10
• IFI10
• INP10
• IP-10
• mob-1
• SCYB10

Background

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
3. Sgadari, C. et al. (1996) Blood, 87:3877.

Publications for CXCL10/IP-10/CRG-2 (466-CR)(18)

Publications using 466-CR

• Seynhaeve, ALB;Liu, H;Priester, MI;Valentijn, M;van Holten-Neelen, C;Brouwer, RWW;van Brakel, M;Dik, WA;van IJcken, WFJ;Debets, R;Stubbs, AP;Ten Hagen, TLM; CXCL10 Secreted by Pericytes Mediates TNF?-Induced Vascular Leakage in Tumors and Enhances Extravasation of Nanoparticle-Based Chemotherapeutics Cancer research 2025-02-26 [PMID: 40009768] (In vivo assay, Mouse)
• M Hashimoto, K Araki, MA Cardenas, P Li, RR Jadhav, HT Kissick, WH Hudson, DJ McGuire, RC Obeng, A Wieland, J Lee, DT McManus, JL Ross, SJ Im, J Lee, JX Lin, B Hu, EE West, CD Scharer, GJ Freeman, AH Sharpe, SS Ramalingam, A Pellerin, V Teichgräbe, WJ Greenleaf, C Klein, JJ Goronzy, P Umaña, WJ Leonard, KA Smith, R Ahmed PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program Nature, 2022-09-28;610(7930):173-181. 2022-09-28 [PMID: 36171288] (Bioassay, Mouse)
• JA Carroll, BR Groveman, K Williams, R Moore, B Race, CL Haigh Prion protein N1 cleavage peptides stimulate microglial interaction with surrounding cells Sci Rep, 2020-04-20;10(1):6654. 2020-04-20 [PMID: 32313035] (Bioassay, Mouse)
• YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy J. Clin. Invest., 2019-07-16;130(0):. 2019-07-16 [PMID: 31310587] (Bioassay, Mouse)
• S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019-06-25;10(0):1421. 2019-06-25 [PMID: 31293551] (Binding Assay, Mouse)
• Q Zhao, T Kim, J Pang, W Sun, X Yang, J Wang, Y Song, H Zhang, H Sun, V Rangan, S Deshpande, H Tang, ME Cvijic, R Westhouse, T Olah, J Xie, M Struthers, L Salter-Cid A novel function of CXCL10 in mediating monocyte production of proinflammatory cytokines J. Leukoc. Biol., 2017-09-12;0(0):. 2017-09-12 [PMID: 28899907] (In Vivo, Mouse)
• S Geng, Y Zhong, X Zhou, G Zhao, X Xie, Y Pei, H Liu, H Zhang, Y Shi, B Wang Induced Regulatory T Cells Superimpose Their Suppressive Capacity with Effector T Cells in Lymph Nodes via Antigen-Specific S1p1-Dependent Egress Blockage Front Immunol, 2017-06-07;8(0):663. 2017-06-07 [PMID: 28638384] (Bioassay, Mouse)
• EM Putz, AJ Mayfosh, K Kos, DS Barkauskas, K Nakamura, L Town, KJ Goodall, DY Yee, IK Poon, N Baschuk, F Souza-Fons, MD Hulett, MJ Smyth NK cell heparanase controls tumor invasion and immune surveillance J. Clin. Invest., 2017-06-05;0(0):. 2017-06-05 [PMID: 28581441] (Bioassay, Mouse)
• GM Castillo, A Nishimoto-, CC Jones, KK Kabirov, A Zakharov, AV Lyubimov Protected graft copolymer-formulated fibroblast growth factors mitigate the lethality of partial body irradiation injury PLoS ONE, 2017-02-16;12(2):e0171703. 2017-02-16 [PMID: 28207794] (ELISA (Standard), Mouse)
• Ioannidis L, Nie C, Ly A, Ryg-Cornejo V, Chiu C, Hansen D Monocyte- and Neutrophil-Derived CXCL10 Impairs Efficient Control of Blood-Stage Malaria Infection and Promotes Severe Disease. J Immunol, 2015-12-30;196(3):1227-38. 2015-12-30 [PMID: 26718341] (ELISA (Standard), Mouse)
• Mitagami Y, Yasunaga J, Kinosada H, Ohshima K, Matsuoka M Interferon-gamma Promotes Inflammation and Development of T-Cell Lymphoma in HTLV-1 bZIP Factor Transgenic Mice. PLoS Pathog, 2015-08-21;11(8):e1005120. 2015-08-21 [PMID: 26296091] (Bioassay, Mouse)
• Clarner T, Janssen K, Nellessen L, Stangel M, Skripuletz T, Krauspe B, Hess F, Denecke B, Beutner C, Linnartz-Gerlach B, Neumann H, Vallieres L, Amor S, Ohl K, Tenbrock K, Beyer C, Kipp M CXCL10 triggers early microglial activation in the cuprizone model. J Immunol, 2015-02-27;194(7):3400-13. 2015-02-27 [PMID: 25725102] (Bioassay, Mouse)
• Cuenca A, Joiner D, Gentile L, Cuenca A, Wynn J, Kelly-Scumpia K, Scumpia P, Behrns K, Efron P, Nacionales D, Lui C, Wallet S, Reeves W, Mathews C, Moldawer L TRIF-dependent innate immune activation is critical for survival to neonatal gram-negative sepsis. J Immunol, 2014-12-29;194(3):1169-77. 2014-12-29 [PMID: 25548220] (Bioassay, Mouse)
• Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer&#039;s disease model. J Clin Invest, 2014-12-15;125(1):365-78. 2014-12-15 [PMID: 25500888] (Bioassay, Mouse)
• Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014-05-01;32(5):1208-19. 2014-05-01 [PMID: 24446149] (Bioassay, Human, Mouse)
• Lacotte S, Decossas M, Le Coz C, Brun S, Muller S, Dumortier H Early differentiated CD138(high) MHCII+ IgG+ plasma cells express CXCR3 and localize into inflamed kidneys of lupus mice. PLoS ONE, 2013-03-08;8(3):e58140. 2013-03-08 [PMID: 23520491] (Bioassay, Mouse)
• Kim HS, Go H, Akira S, Chung DH TLR2-mediated production of IL-27 and chemokines by respiratory epithelial cells promotes bleomycin-induced pulmonary fibrosis in mice. J. Immunol., 2011-09-19;187(8):4007-17. 2011-09-19 [PMID: 21930967] (In Vivo, Mouse)
• Liu C, Luo D, Reynolds BA Chemokine receptor CXCR3 promotes growth of glioma. Carcinogenesis, 2010-11-03;32(2):129-37. 2010-11-03 [PMID: 21051441] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Cxcl10
• Uniprot:
  • Q548V9()