Recombinant Mouse CXCL1/KC Protein

Images

 
There are currently no images for .

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications BA, BA2

Order Details

Recombinant Mouse CXCL1/KC Protein Summary

Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.3-0.9 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 3-15 ng/mL.
Source
E. coli-derived
Arg20-Lys96
Accession #
N-terminal Sequence
Arg20
Protein/Peptide Type
Recombinant Proteins
Gene
Cxcl1
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
453-KC in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL1/KC Protein

  • CINC1
  • CINC-1
  • CXCL1
  • GRO alpha
  • KC
  • MGSA-alpha

Background

CXCL1, also known as KC, GRO alpha, and CINC-1, is an approximately 8 kDa proinflammatory chemokine that plays a key role in neutrophil migration and activation (1). Mature mouse CXCL1 shares 64% and 92% aa sequence identity with human and rat CXCL1, respectively [oquendo 4133, ryseck 266, cochran 939]. It is produced by many cell types in inflammatory sites and during chronic inflammatory diseases (1). CXCL1 can associate into bioactive dimers and primarily signals through CXCR2/IL-8 RB but can also bind with lower affinity to CXCR2/IL-8 RA (5-7). It induces neutrophil migration, extravasation, respiratory burst, and degranulation and also induces T cells to produce proinflammatory IL-17 (6, 8, 9). CXCL1 additionally binds to Syndecan-1 on epithelial cells which acts as a sink for CXCL1 activity until Syndecan-1 cleavage by MMP-7 (10). CXCL1 is up-regulated in spinal cord astrocytes by inflammatory stimuli or tumor cell injection, and it exacerbates pain sensation by potentiating excitatory NMDA neurotransmission (11, 12). In the circulatory system, CXCL1 interacts with CXCR2 on endothelial cells to promote lymphatic tube formation and angiogenesis (13, 14). It promotes the hypertrophic differentiation of chondrocytes resulting in cartilage matrix deposition, calcification, and remodeling (15). It interacts with both CXCR1 and CXCR2 on adipose stromal cells and promotes their recruitment to prostate tumors in obese patients (16). It also binds CXCR2 on ovarian cancer cells, leading to cleavage of cell surface HB-EGF, transactivation of EGF R, and cell proliferation (17).

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NBP1-05164
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-Fr, IHC-P
MAB331
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
MAB393
Species: Hu
Applications: WB, Simple Western, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
AF3667
Species: Hu, Mu
Applications: WB, Simple Western, IHC, ICC
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
MAB330
Species: Hu
Applications: Flow, IHC, CyTOF-reported, Neut

Publications for (43)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA Standard, In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(12)
ELISA Standard
(9)
In Vivo
(18)
All Applications
Filter By Species
Human
(2)
Mouse
(29)
N/A
(9)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 453-KC Applications Species
J García-Pri, R Villena-Gu, M Gómez, E Bernardo, A Pun-García, I García-Lun, G Crainiciuc, R Fernández-, V Sreeramkum, R Bourio-Mar, JM García-Rui, AS Del Valle, D Sanz-Rosa, G Pizarro, A Fernández-, A Hidalgo, V Fuster, B Ibanez Neutrophil stunning by metoprolol reduces infarct size Nat Commun, 2017;8(0):14780. 2017 [PMID: 28416795] (Bioassay, Mouse) Bioassay Mouse
T Ishiguro, T Fukawa, K Akaki, K Nagaoka, T Takeda, Y Iwakura, K Inaba, K Takahara Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28127756] (Bioassay, Mouse) Bioassay Mouse
J Latasiewic, A Artz, D Jing, MP Blanco, SM Currie, MV Avila, M Schnoor, D Vestweber HS1 deficiency impairs neutrophil recruitment in vivo and activation of the small GTPases Rac1 and Rap1 J. Leukoc. Biol, 2017;0(0):. 2017 [PMID: 28122813] (In Vivo, Mouse) In Vivo Mouse
iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation Cell Rep, 2016;16(12):3260-72. 2016 [PMID: 27653688] (In Vivo, Mouse) In Vivo Mouse
Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype PLoS ONE, 2016;11(8):e0159986. 2016 [PMID: 27525971] (Bioassay, Mouse) Bioassay Mouse
Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow. J Immunol, 2015;195(8):3880-9. 2015 [PMID: 26355151] (Bioassay, Mouse) Bioassay Mouse
Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014;32(5):1208-19. 2014 [PMID: 24446149] (Bioassay, Human) Bioassay Human
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013;190(10):5329-36. 2013 [PMID: 23589623] (Bioassay, Mouse) Bioassay Mouse
Aeffner F, Davis I Respiratory syncytial virus reverses airway hyperresponsiveness to methacholine in ovalbumin-sensitized mice. PLoS ONE, 2012;7(10):e46660. 2012 [PMID: 23056391] (In Vivo, Mouse) In Vivo Mouse
Reichel CA, Puhr-Westerheide D, Zuchtriegel G, Uhl B, Berberich N, Zahler S, Wymann MP, Luckow B, Krombach F C-C motif chemokine CCL3 and canonical neutrophil attractants promote neutrophil extravasation through common and distinct mechanisms. Blood, 2012;120(4):880-90. 2012 [PMID: 22674804] (In Vivo, Mouse) In Vivo Mouse
Show All 43 Publications.

Reviews for CXCL1/GRO alpha/KC/CINC-1 (0)

There are no reviews for CXCL1/GRO alpha/KC/CINC-1. By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for CXCL1/GRO alpha/KC/CINC-1 (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional CXCL1/GRO alpha/KC/CINC-1 Products

Bioinformatics Tool for CXCL1/GRO alpha/KC/CINC-1

Discover related pathways, diseases and genes to CXCL1/GRO alpha/KC/CINC-1. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CXCL1/GRO alpha/KC/CINC-1

Discover more about diseases related to CXCL1/GRO alpha/KC/CINC-1.
 

Pathways for CXCL1/GRO alpha/KC/CINC-1

View related products by pathway.

PTMs for CXCL1/GRO alpha/KC/CINC-1

Learn more about PTMs related to CXCL1/GRO alpha/KC/CINC-1.

Blogs on CXCL1/GRO alpha/KC/CINC-1

There are no specific blogs for CXCL1/GRO alpha/KC/CINC-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Mouse CXCL1/KC Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol Cxcl1
Entrez
Uniprot