Recombinant Mouse CXCL1/KC Protein


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Recombinant Mouse CXCL1/KC Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 3-15 ng/mL.
E. coli-derived mouse CXCL1/GRO alpha/KC/CINC-1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
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453-KC in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, -20 to -70 °C under sterile conditions after reconstitution.
  • 3 months, -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CXCL1/KC Protein

  • CINC1
  • CINC-1
  • CXCL1
  • GRO alpha
  • KC
  • MGSA-alpha


CXCL1, also known as KC, GRO alpha, and CINC-1, is an approximately 8 kDa proinflammatory chemokine that plays a key role in neutrophil migration and activation (1). Mature mouse CXCL1 shares 64% and 92% aa sequence identity with human and rat CXCL1, respectively [oquendo 4133, ryseck 266, cochran 939]. It is produced by many cell types in inflammatory sites and during chronic inflammatory diseases (1). CXCL1 can associate into bioactive dimers and primarily signals through CXCR2/IL-8 RB but can also bind with lower affinity to CXCR2/IL-8 RA (5-7). It induces neutrophil migration, extravasation, respiratory burst, and degranulation and also induces T cells to produce proinflammatory IL-17 (6, 8, 9). CXCL1 additionally binds to Syndecan-1 on epithelial cells which acts as a sink for CXCL1 activity until Syndecan-1 cleavage by MMP-7 (10). CXCL1 is up-regulated in spinal cord astrocytes by inflammatory stimuli or tumor cell injection, and it exacerbates pain sensation by potentiating excitatory NMDA neurotransmission (11, 12). In the circulatory system, CXCL1 interacts with CXCR2 on endothelial cells to promote lymphatic tube formation and angiogenesis (13, 14). It promotes the hypertrophic differentiation of chondrocytes resulting in cartilage matrix deposition, calcification, and remodeling (15). It interacts with both CXCR1 and CXCR2 on adipose stromal cells and promotes their recruitment to prostate tumors in obese patients (16). It also binds CXCR2 on ovarian cancer cells, leading to cleavage of cell surface HB-EGF, transactivation of EGF R, and cell proliferation (17).
  1. Strieter, R.M. et al. (2005) Cytokine Growth Factor Rev. 16:593.
  2. Cochran, B.H. et al. (1983) Cell 33:939.
  3. Oquendo, P. et al. (1989) J. Biol. Chem. 264:4133.
  4. Ryseck, R.P. et al. (1989) Exp. Cell Res. 180:266.
  5. Sawant, K.V. et al. (2015) J. Innate Immun. 7:647.
  6. Geiser, T. et al. (1993) J. Biol. Chem. 268:15419.
  7. Ahuja, S.K. and P.M. Murphy (1996) J. Biol. Chem. 271:20545.
  8. Jin, L. et al. (2014) J. Immunol. 193:3549.
  9. De Filippo, K. et al. (2013) Blood 121:4930.
  10. Gill, S.E. et al. (2016) Am. J. Respir. Cell. Mol. Biol. PMID 26934670.
  11. Cao, D.-L. et al. (2014) Exp. Neurol. 261:328.
  12. Xu, J. et al. (2014) J. Neuroinflamm. 11:38.
  13. Xu, J. et al. (2012) Int. J. Cancer 130:787.
  14. Miyake, M. et al. (2013) Lab. Invest. 93:768.
  15. Merz, D. et al. (2003) J. Immunol. 171:4406.
  16. Zhang, T. et al. (2016) Nat. Commun. 7:11674.
  17. Bolitho, C. et al. (2010) Endocr. Relat. Cancer 17:929.

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Publications for CXCL1/GRO alpha/KC/CINC-1 (453-KC)(45)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Binding Assay, Bioassay, ELISA Standard, In Vivo.

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Showing Publications 1 - 10 of 45. Show All 45 Publications.
Publications using 453-KC Applications Species
TA Pádua, ND Torres, ALP Candéa, MFS Costa, JD Silva, JL Silva-Filh, FTM Costa, PRM Rocco, MC Souza, MG Henriques Therapeutic effect of Lipoxin A4 �in malaria-induced acute lung injury J. Leukoc. Biol., 2018;0(0):. 2018 [PMID: 29345368] (Bioassay, Mouse) Bioassay Mouse
J García-Pri, R Villena-Gu, M Gómez, E Bernardo, A Pun-García, I García-Lun, G Crainiciuc, R Fernández-, V Sreeramkum, R Bourio-Mar, JM García-Rui, AS Del Valle, D Sanz-Rosa, G Pizarro, A Fernández-, A Hidalgo, V Fuster, B Ibanez Neutrophil stunning by metoprolol reduces infarct size Nat Commun, 2017;8(0):14780. 2017 [PMID: 28416795] (Bioassay, Mouse) Bioassay Mouse
T Ishiguro, T Fukawa, K Akaki, K Nagaoka, T Takeda, Y Iwakura, K Inaba, K Takahara Absence of DCIR1 reduces the mortality rate of endotoxemic hepatitis in mice Eur. J. Immunol, 2017;0(0):. 2017 [PMID: 28127756] (Bioassay, Mouse) Bioassay Mouse
J Latasiewic, A Artz, D Jing, MP Blanco, SM Currie, MV Avila, M Schnoor, D Vestweber HS1 deficiency impairs neutrophil recruitment in vivo and activation of the small GTPases Rac1 and Rap1 J. Leukoc. Biol, 2017;0(0):. 2017 [PMID: 28122813] (In Vivo, Mouse) In Vivo Mouse
Clara Jana-Lui Busch Malondialdehyde epitopes are sterile mediators of hepatic inflammation in hypercholesterolemic mice Hepatology, 2016;0(0):. 2016 [PMID: 27981604] (ELISA Standard) ELISA Standard
iNKT Cell Emigration out of the Lung Vasculature Requires Neutrophils and Monocyte-Derived Dendritic Cells in Inflammation Cell Rep, 2016;16(12):3260-72. 2016 [PMID: 27653688] (In Vivo, Mouse) In Vivo Mouse
Allostimulatory Effects of Dendritic Cells with Characteristic Features of a Regulatory Phenotype PLoS ONE, 2016;11(8):e0159986. 2016 [PMID: 27525971] (Bioassay, Mouse) Bioassay Mouse
Yago T, Tsukamoto H, Liu Z, Wang Y, Thompson L, McEver R Multi-Inhibitory Effects of A2A Adenosine Receptor Signaling on Neutrophil Adhesion Under Flow. J Immunol, 2015;195(8):3880-9. 2015 [PMID: 26355151] (Bioassay, Mouse) Bioassay Mouse
Fujihara Y, Takato T, Hoshi K Macrophage-inducing FasL on chondrocytes forms immune privilege in cartilage tissue engineering, enhancing in vivo regeneration. Stem Cells, 2014;32(5):1208-19. 2014 [PMID: 24446149] (Bioassay, Human) Bioassay Human
Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis. J Immunol, 2013;190(10):5329-36. 2013 [PMID: 23589623] (Bioassay, Mouse) Bioassay Mouse
Show All 45 Publications.

Review for CXCL1/GRO alpha/KC/CINC-1 (453-KC) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using 453-KC:
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Gene Symbol Cxcl1