Recombinant Mouse CKAP4/p63 Protein, CF

When Recombinant Mouse Dkk‑1 (Catalog # 5897-DK) isimmobilized at 1 μg/mL (100 μL/well), Recombinant Mouse CKAP4/p63 binds with anED50 of 35-280 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CKAP4/p63 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Dkk‑1 (Catalog # 5897-DK) is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CKAP4/p63 that produces 50% of the optimal binding response is 35-280 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse CKAP4/p63 protein
  Val109-Ile575, with an N-terminal 6-His tag
• Accession #: AAH25522
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 45-59 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CKAP4/p63 Protein, CF

• 63 kDa membrane protein
• CKAP4
• CLIMP-63
• cytoskeleton-associated protein 4
• ERGIC-63
• MGC99554
• p63
• transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediatecompartment
• type-II transmembrane protein p63

Background

CKAP4 (Cytoskeleton-associated protein 4; also CLIMP63 and p63) is a 63-64 kDa molecule that belongs to no known protein family. It is found intracellularly, and on the plasma membrane of select cells such as vascular smooth muscle and Type II Greater alveolar lung cells (1). CKAP4 has a bimodal distribution. First, it is embedded in the membrane of a compartment that links the ER with the Golgi apparatus (2). This localization is dependent upon its ability to form homooligomers, and its presence serves to anchor microtubules and direct the formation of tubular ER. Second, it is embedded in the plasma membrane and serves as a receptor for SP‑A/surfactant protein-A (in lung) (2) and tPA (in vessels) (3). Mouse CKAP4 is a 575 amino acid (aa) type II transmembrane nonglycosylated protein. It contains an 85 aa N-terminal cytoplasmic region plus a 467 aa C-terminal luminal domain (aa 109-575) (4). The luminal domain contains three coiled-coil regions (aa 125-193; 236‑438; 507-575) plus three utilized phosphorylation sites (5). CKAP4 undergoes reversible palmitoylation (6). Over aa 109-575, mouse CKAP4 shares 82% and 94% aa sequence identity with human and rat CKAP4, respectively. CKAP4 has been shown to be required for APF-mediated signaling (6-9), for gentamycin-induced cytotoxicity (8), for regulation of tPA function (3), and for SP-A-induced surfactant uptake by pneumocytes (10). CKAP4 interacts with Dickkopf-1 (Dkk-1) to promote activation of AKT-dependent, Wnt-independent signaling(11). Both CKAP4 and Dkk-1 are frequently up-regulated in pancreatic and lung cancers (11). Dkk-1 binds to CKAP4 with high affinity that is comparable to but independent of its interaction with LRP5/6 (11).

1. Bates, S. R. et al. (2008) Am J Physiol Lung Cell Mol Physiol, 295:L658.
2. Gupta, N. et al. (2006). Am J Physiol Lung Cell Mol Physiol 291:L436.
3. Razzaq, T. M. et al. (2003). J. Biol. Chem. 278:42679.
4. Schweizer, A. et al. (1994). J. Cell Biol.126:25.
5. Huttlin, E. L. et al. (2010) Cell 143:1174.
6. Planey, S. L. et al. (2009). Mol Biol Cell. 20:1454.
7. Conrads, T.P. (2006) J Bio Chem, 281:49.
8. Matika, C.A. (2012) Mol Bio Cell, 23:10.
9. Shahjee, H.M. (2010) J Exp and Clin Cancer Res, 29:1.
10. Kazi, A.S. (2010) Am J Physiol Lung Cell Mol Physiol 299:6.
11. Kimura, H. et al. (2016) J Clin Invest, 126:2689.

Bioinformatics

• Uniprot:
  • AAH25522()