Recombinant Mouse CD83 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD83 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of human monocyte-derived dendritic cells. When 5 x 10⁴ cells/well are added to mouse CD83/Fc Chimera coated plates (2.5 µg/mL with 100 µL/well), approximately 50-75% will adhere after 1 hour incubation at room temperature.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse CD83 protein
  

  Mouse CD83 (Met22 - Ala134) Accession #NP_033986	DIEGRMD	Human IgG1 (Pro100 - Lys330)
  N-terminus		C-terminus

• Accession #: NP_033986
• N-terminal Sequence: Met22
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd83
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 39.1 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 52 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD83 Fc Chimera Protein, CF

• B-cell activation protein
• BL11
• BL11CD83 antigen
• CD83 antigen (activated B lymphocytes, immunoglobulin superfamily)
• CD83 molecule
• CD83
• Cell surface protein HB15
• cell-surface glycoprotein
• HB15
• HB15hCD83

Background

Mouse CD83 is a 30 - 35 kDa member of the Siglec (or sialic-acid-binding immunoglobulin-like lectin) family of transmembrane proteins (1, 2, 3). CD83 is synthesized as a type I transmembrane glycoprotein that contains a 114 amino acid (aa) extracellular region, a 22 aa transmembrane segment, and a 39 aa cytoplasmic domain. It contains one V type Ig-like domain in the extracellular region with no inhibitory cytoplasmic motif(s). In the extracellular region, mouse and human CD83 are 66% aa identical (1, 2, 4). Relative to mouse, human CD83 has an 11 aa insertion in its extracellular domain and is expressed as a 45 - 55 kDa protein (1, 4, 5, 6). No alternate splice variants have been reported for mouse. In human, however, one soluble splice form has been reported and proteolytic processing is suggested to generate a second circulating isoform (6, 7). Notably, although soluble CD83 has the potential to exist as either a monomer or disulfide-linked dimer, both show immunosuppressive activity (4, 8, 9). Membrane CD83, by contrast, is immunostimulatory (10). CD83 is a primary marker for dendritic cells (3, 5, 6). It is also found on B cells (6, 11), neutrophils (12), monocytes and macrophages (13). Except for dendritic cells, CD83 expression is often transient. CD83 binds to sialic acids on monocytes (3). The function of CD83 is only now becoming clear. As noted, membrane-immobilized CD83 appears to promote T cell proliferation, particularly of CD8⁺ cytotoxic T cells (14). On monocytes, CD83 may also drive monocytes into a fibrocyte phenotype (14). And a lack of membrane-expressed CD83 leads to an unusual IL-4/IL-10 producing CD4⁺ T cell phenotype (15).

1. Berchtold, S. et. al. (1999) FEBS Lett. 461:211.
2. Fujimoto, Y. and T.F. Tedder (2006) J. Med. Dent. Sci. 53:85.
3. Scholler, N. et. al. (2001) J. Immunol. 166:3865.
4. Lechmann, M. et al. (2005) Biochem. Biophys. Res. Commun. 329:132.
5. Zhou, L-J. et. al. (1992) J. Immunol. 149:735.
6. Hock, B.D. et al. (2001) Int. Immunol. 13:959.
7. Dudziak, D. et al. (2005) J. Immunol. 174:6672.
8. Kotzor, N. et al. (2004) Immunobiology 209:129.
9. Zinser, E. et al. (2006) Immunobiology 21:449.
10. Hirano, N. et al. (2006) Blood 107:1528.
11. Cramer, S.O. et al. (2000) Int. Immunol. 12:1347.
12. Yamashiro, S. et al. (2000) Blood 96:3958.
13. Cao, W. et al. (2005) Biochem. J. 385:85.
14. Scholler, N. et al. (2002) J. Immunol. 168:2599.
15. Garcia-Martinez, L.F. et al. (2004) J. Immunol. 173:2995.

Bioinformatics

• Gene Symbol: Cd83
• Uniprot:
  • NP_033986()