Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED₅₀ for this effect is 0.75-3.5 μg/mL.
  Optimal dilutions should be determined by each laboratory for each application.
• Source: Chinese Hamster Ovary cell line, CHO-derived mouse CD300e/LMIR6 protein
  

  Mouse CD300E (Leu18-Leu166) Accession # EDL34457	IEGRMDP	Mouse IgG2A (Glu98-Lys330)
  N-terminus		C-terminus

• Accession #: EDL34457
• N-terminal Sequence: Leu18
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Cd300e
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 43.6 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 55-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CD300e/LMIR6 Fc Chimera Protein, CF

• CD300 antigen like family member E
• CD300 antigen-like family member E
• CD300e antigenPIgR-2
• CD300e molecule
• CD300e
• CD300LE
• CD300LEPIgR2
• CLM2
• CLM-2
• CLM2CMRF35-A5
• CMRF35A5
• CMRF35-like molecule 2
• Immune receptor expressed on myeloid cells 2
• IREM-2
• IREM2IREM-2
• LMIR6
• Poly-Ig receptor 2
• Polymeric immunoglobulin receptor 2

Background

CD300E, also known as LMIR6, IREM-2, and CMRF35-like 2 (CLM-2), is an approximately 35 kDa type I transmembrane glycoprotein in the LMIR family of immune regulatory proteins (1). It consists of a 154 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 4 aa cytoplasmic stub (2). Within the ECD, mouse CD300E shares 57% and 78% aa sequence identity with human and rat CD300E, respectively. CD300E is expressed on monocytes and dendritic cells (3, 4). Its transmembrane segment contains a charged lysine residue which mediates CD300E association with the signaling adaptor molecule DAP12 and enables CD300E to function as an activating receptor (2, 3). Antibody crosslinking of CD300E promotes monocyte and dendritic cell survival, production of inflammatory chemokines and cytokines, upregulation of costimulatory molecules, and also the ability of dendritic cells to induce T cell proliferation (4).

1. Clark, G.J. et al. (2009) Trends Immunol. 30:209.
2. Chung, D.H. et al. (2003) J. Immunol. 141:6541.
3. Aguilar, H. et al. (2004) J. Immunol. 173:6703.
4. Brckalo, T. et al. (2010) Eur. J. Immunol. 40:722.

Bioinformatics

• Gene Symbol: Cd300e
• Uniprot:
  • EDL34457()