Recombinant Mouse CCL6/C10 Protein

Recombinant Mouse CCL6/C10 (Catalog # 487-C)chemoattracts BaF3 mouse pro B cells transfected with human CCR1. The ED50 for this effect is 50-250 ng/mL.

• Reactivity: Mu
• Applications: Bioactivity

Recombinant Mouse CCL6/C10 Protein Summary

• Details of Functionality: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR1. The ED₅₀ for this effect is 50-250 ng/mL.
• Source: E. coli-derived mouse CCL6/C10 protein
  Gly22-Ala116
• Accession #: P27784
• N-terminal Sequence: Gly22
• Protein/Peptide Type: Recombinant Proteins
• Gene: Ccl6
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 10.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL6/C10 Protein

• C10
• c10;C-C motif chemokine 6;MRP;MRP-1;Scy;Scya6
• CCL6
• MRP-1
• SCYA6

Background

Mouse CCL6, a member of the beta subfamily of chemokines, was initially identified as a transcript that is induced in bone marrow cells upon stimulation with GM-CSF. A human homologue for mouse CCL6 has not been identified. The mCCL6 cDNA encodes a 116 amino acid residue precursor protein with a hydrophobic signal peptide that is cleaved to yield a 95 amino acid residue mature protein. Compared to other chemokines, mCCL6 has a large N-terminal extension. The expression of CCL6 mRNA has been detected in mouse monocytes and neutrophils, where it is strongly induced upon GM-CSF stimulation. CCL6 expression has also been detected in an IL-2-dependent T cell line, where the expression is inhibited following T cell stimulation. Mouse CCL6 expression was also shown to be strongly induced by IL-4 in mouse macrophages. The gene for CCL6 has been mapped to mouse chromosome 11. The E. coli expressed CCL6 produced at R&D Systems has been shown to be a monocyte chemoattractant.

Publications for CCL6/C10 (487-C)(2)

Publications using 487-C

• Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa. Mucosal Immunol, 2009-10-07;3(1):40-8. 2009-10-07 [PMID: 19812544] (Bioassay, ELISA (Standard), Mouse)
• Ueno T, Kuse S, Saito F, Nitta T, Kakiuchi T, Hollander GA, Takahama Y The role of CCL21 in recruitment of T-precursor cells to fetal thymi. Blood, 2004-09-09;105(1):31-9. 2004-09-09 [PMID: 15358618] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: Ccl6
• Uniprot:
  • P27784()