POLR3K Antibody - BSA Free

Immunohistochemistry: POLR3K Antibody - BSA Free [NBP3-46938] - Immunohistochemistry of paraffin-embedded human gliomas using (POLR3K antibody) at dilution of 1:100

Western Blot: POLR3K Antibody - BSA Free [NBP3-46938] - Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with (POLR3K Antibody) at dilution of 1:1000

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

POLR3K Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit POLR3K Antibody - BSA Free (NBP3-46938) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: polymerase(RNA) III(DNA directed) polypeptide K, 12.3 kDa. (Uniprot# Q9Y2Y1)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: POLR3K
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:500
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:5000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for POLR3K Antibody - BSA Free

• C11
• DNA-directed RNA polymerase III subunit K
• DNA-directed RNA polymerase III subunit RPC10
• DNA-directed RNA polymerases III 12.5 kDa polypeptide
• hRPC11
• HsC11p
• polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa)
• polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
• RNA polymerase III 12.5 kDa subunit
• RNA polymerase III subunit (hRPC11)
• RNA polymerase III subunit C10
• RNA polymerase III subunit C11
• RNA polymerase III subunit CII
• RPC10
• RPC11C11-RNP3
• RPC12.5

Background

POLR3K encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR3K