CCL6/C10 Products

487-C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemo-taxis elicited by Recombinant Mouse CCL6/C10 (1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB487). The ND₅₀ is typically 6-24 µg/mL." class="big_lightbox" target="_blank">

487-C) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL6/C10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL6/C10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF487). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox" target="_blank">

487-C) was serially diluted and captured by Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4871) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse CCL6/C10 Monoclonal Antibody (Catalog # MAB4872) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">