Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119), CF

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Summary
Reactivity MuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119), CF Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with mouse CCR3. The ED50 for this effect is 150-600 ng/mL.
In the same assay, the ED50 of the aa 27-119 mouse Eotaxin-2 variant (R&D Systems, Catalog # 1017-MP) is 1-5 ng/mL. The aa 24-119 mouse Eotaxin-2 variant is about 150-fold less active than the aa 27-119 mouse Eotaxin-2 variant.
Source
E. coli-derived mouse CCL24/Eotaxin-2/MPIF-2 protein
Ile24-Val119
Accession #
N-terminal Sequence
Ile24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl24
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
10.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL24/Eotaxin-2/MPIF-2 (aa 24-119), CF

  • CCL24
  • chemokine (C-C motif) ligand 24
  • Ckb-6
  • Eosinophil chemotactic protein 2
  • Eotaxin-2
  • member 24
  • MPIF2
  • MPIF-2
  • MPIF2Myeloid progenitor inhibitory factor 2
  • MPIF-2Small-inducible cytokine A24
  • SCYA24CK-beta-6

Background

Eotaxin-2, also named myeloid progenitor inhibitory factor (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. LPS and IL-4 also differentially regulate the expression of Eotaxin-2 in monocytes and macrophages. Mouse Eotaxin-2 cDNA encodes a 119 amino acid (aa) residue precursor protein that shares approximately 58% aa sequence identity with human Eotaxin-2. Functionally, Eotaxin-2 is most closely related to eotaxin/CCL11 and Eotaxin-3/CCL26. The three proteins share low sequence homology but have been shown to be potent eosinophil chemoattractants that bind and activate the chemokine receptor CCR3, a receptor that is highly expressed in eosinophils. Eotaxin-2 also has the ability to suppress myeloid cell proliferation, a biological function not shared by eotaxin. Two amino-terminally-truncated isoforms of mouse Eotaxin-2 have been produced at R&D Systems. The Val 27 - Val 119 mouse Eotaxin-2 isoform has been shown to be 150-fold more potent than the Ile 24 - Val 119 isoform as a chemoattractant for mouse BaR/3 cells transfected with mouse CCR3 (1 - 3).

  1. Zimmermann, N. et al. (2000) J. Immunol. 165:5839.
  2. Watanabe, K. et al. (2002) J. Immunol. 168:1911.
  3. Grzegorzewski, K.J. et al. (2001) Cytokine 13:209.

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Bioinformatics

Gene Symbol Ccl24
Uniprot