Recombinant Mouse CCL2/JE/MCP-1 Protein

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1 μg/lane of Recombinant Mouse CCL2/JE/MCP-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 8 kDa.
Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A. The ED50 for this effect is 2-10 ng/mL.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Bioactivity

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Recombinant Mouse CCL2/JE/MCP-1 Protein Summary

Details of Functionality
Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR2A. The ED50 for this effect is typically 2-10 ng/mL.
Source
E. coli-derived mouse CCL2/JE/MCP-1 protein
Gln24-Arg96
Accession #
N-terminal Sequence
Gln24
Protein/Peptide Type
Recombinant Proteins
Gene
Ccl2
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
8.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
479-JE in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse CCL2/JE/MCP-1 Protein

  • C-C motif chemokine ligand 2
  • CCL2
  • GDCF-2
  • HC11
  • HSMCR30
  • MCAF
  • Mcp1
  • MCP-1
  • SCYA2
  • SMC-CF

Background

CCL2, also called monocyte chemotactic protein-1 (MCP-1) or JE, is a member of the C-C or beta  chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Mouse CCL2 cDNA encodes a 148 amino acid (aa) precursor protein with a 23 aa signal peptide and a 125 aa mature protein (1). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding (3). Mouse CCL2 forms a broad band around 25 kDa on SDS-PAGE due to non-covalent dimerization and variable carbohydrate content (1). Mouse and rat express a form of CCL2 that is extended by 49 aa compared to other species. Mature mouse CCL2 shares 82% amino acid (aa) identity with rat CCL2 over the entire sequence, and 58%, 56%, 55%, 53% and 53% aa identity with human, equine, porcine, bovine and canine CCL2, respectively, over aa 24 ‑ 101.  Human CCL2 can, however, induce a limited response in rodent cells, and mouse CCL2 has full activity on human cells (2, 4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7). When a DNA sequence encoding the 125 aa residue of the mature CCL2 protein was expressed in E. coli at R&D Systems, the purified protein had the predicted N-terminus but a mass of 8525 Da. The truncation of most of the C-terminal extension could be due either to purification artifact or to post-translational modification. The truncated recombinant CCL2 has a potency similar to that of human MCP-1 in the monocyte chemotaxis assay.

  1. Rollins, B.J. et al. (1988) Proc. Natl. Acad. Sci. USA. 85:3738.
  2. Deshmane, S.L. et al. (2009) J. Interferon Cytokine Res. 29:313.
  3. Dean, R.A. et al. (2008) Blood. 112:3455.
  4. Van Riper, G. et al. (1993) J. Exp. Med. 177:851.
  5. Luther, S.A. and J.G. Cyster (2001) Nat. Immunol. 2:102.
  6. Daly, C. et al. (2003) Microcirculation 10:247.
  7. Aukrust, P. et al. (2008) Arterioscler. Thromb. Vasc. Biol. 28:1909.

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Publications for CCL2/JE/MCP-1 (479-JE)(71)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, N/A.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Binding Assay
(1)
Bioassay
(47)
Cell Culture
(2)
ELISA (Standard)
(6)
In Vivo
(15)
All Applications
Filter By Species
Human
(3)
Mouse
(61)
Rat
(1)
N/A
(6)
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Showing Publications 1 - 10 of 71. Show All 71 Publications.
Publications using 479-JE Applications Species
GB Kitchen, PS Cunningham, TM Poolman, M Iqbal, R Maidstone, M Baxter, J Bagnall, N Begley, B Saer, T Hussell, LC Matthews, DH Dockrell, HJ Durrington, JE Gibbs, JF Blaikley, AS Loudon, DW Ray The clock gene Bmal1 inhibits macrophage motility, phagocytosis, and impairs defense against pneumonia Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):. 2020 [PMID: 31900362] (Cell Culture, Mouse) Cell Culture Mouse
S Sharif, N Ueda, Y Nakatani, LM Wise, S Clifton, Z Lateef, AA Mercer, SB Fleming Chemokine-Binding Proteins Encoded by Parapoxvirus of Red Deer of New Zealand Display Evidence of Gene Duplication and Divergence of Ligand Specificity Front Microbiol, 2019;10(0):1421. 2019 [PMID: 31293551] (Binding Assay, Mouse) Binding Assay Mouse
K Kwiatkowsk, K Popiolek-B, A Piotrowska, E Rojewska, K Ciapa?a, W Makuch, J Mika Chemokines CCL2 and CCL7, but not CCL12, play a significant role in the development of pain-related behavior and opioid-induced analgesia Cytokine, 2019;119(0):202-213. 2019 [PMID: 31003094] (In Vivo, Mouse) In Vivo Mouse
M Qiu, K Huang, Y Liu, Y Yang, H Tang, X Liu, C Wang, H Chen, Y Xiong, J Zhang, J Yang Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis Mucosal Immunol, 2019;0(0):. 2019 [PMID: 30755716] (Bioassay, Mouse) Bioassay Mouse
Z Ma, X Zhao, M Deng, Z Huang, J Wang, Y Wu, D Cui, Y Liu, R Liu, G Ouyang Bone Marrow Mesenchymal Stromal Cell-Derived Periostin Promotes B-ALL Progression by Modulating CCL2 in Leukemia Cells Cell Rep, 2019;26(6):1533-1543.e4. 2019 [PMID: 30726736] (Bioassay, Mouse) Bioassay Mouse
PLD Silva, F Lauretti-F, M Caldas de, SS Lima, AE Covarrubia, M De Franco, E Carvalho, PL Ho, RMA da Costa, EAL Martins, JB Da Silva Phagocytosis of Leptospira by leukocytes from mice with different susceptibility to leptospirosis and possible role of chemokines BMC Microbiol., 2019;19(1):4. 2019 [PMID: 30616505] (Bioassay, Mouse) Bioassay Mouse
T Kubota, M Inoue, N Kubota, I Takamoto, T Mineyama, K Iwayama, K Tokuyama, M Moroi, K Ueki, T Yamauchi, T Kadowaki Downregulation of macrophage Irs2 by hyperinsulinemia impairs IL-4-indeuced M2a-subtype macrophage activation in obesity Nat Commun, 2018;9(1):4863. 2018 [PMID: 30451856] (Bioassay, Mouse) Bioassay Mouse
S Lee, JS Kong, S You, HM Kwon, SA Yoo, CS Cho, WU Kim Transcription Factor NFAT5 Promotes Migration and Invasion of Rheumatoid Synoviocytes via Coagulation Factor III and CCL2 J. Immunol., 2018;0(0):. 2018 [PMID: 29794013] (Bioassay, Human) Bioassay Human
A Zou, N Magee, F Deng, S Lehn, C Zhong, Y Zhang Hepatocyte nuclear receptor SHP suppresses inflammation and fibrosis in a mouse model of nonalcoholic steatohepatitis J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29666185] (Bioassay, Mouse) Bioassay Mouse
J Guo, X Qiu, L Zhang, R Wei Smurf1 regulates macrophage proliferation, apoptosis and migration via JNK and p38 MAPK signaling pathways Mol. Immunol., 2018;97(0):20-26. 2018 [PMID: 29550577] (Bioassay, Mouse) Bioassay Mouse
Show All 71 Publications.

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Bioinformatics

Gene Symbol Ccl2
Uniprot