Recombinant Mouse Axl Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Axl Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Mouse Axl Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Gas6 (Catalog # 986‑GS) that produces 50% optimal binding response is 0.4‑2 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Axl protein
  

  Mouse Axl (His20-Pro443) Accession # Q80YQ3	IEGRMD	Human IgG1 (Pro100-Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: Q80YQ3
• N-terminal Sequence: His20
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Axl
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 73.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-110 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Axl Fc Chimera Protein, CF

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig‑like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Publications for Axl (854-AX)(2)

Publications using 854-AX

• Vandewalle, N;Satilmis, H;Verheye, E;Fan, R;Wang, Y;Groof, T;Bridoux, J;Kerre, T;Beule, N;Becker, A;Bruyne, E;Menu, E;Vanderkerken, K;Breckpot, K;Devoogdt, N;Veirman, K; AXL-specific single domain antibodies show diagnostic potential and anti-tumor activity in Acute Myeloid Leukemia Theranostics 2024-01-01 [PMID: 38773967] (Immunization, Alpaca)
• Rothlin CV, Ghosh S, Zuniga EI, Oldstone MB, Lemke G TAM receptors are pleiotropic inhibitors of the innate immune response. Cell, 2007-12-14;131(6):1124-36. 2007-12-14 [PMID: 18083102] (Bioassay, Mouse)

Review for Axl (854-AX) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Verified Customer Binding assay/Protein-protein interaction 02/26/2018

Summary

• Application: Binding assay/Protein-protein interaction
• Lot: BRY0117011

Bioinformatics

• Gene Symbol: Axl
• Uniprot:
  • Q80YQ3()