Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

• Reactivity: Mu
• Applications: Binding Activity, Bioactivity

Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein Summary

• Details of Functionality: Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED₅₀ for this effect is 0.008-0.05 µg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050). Measured by its ability to bind Recombinant Mouse 4‑1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B) in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived mouse 4-1BB Ligand/TNFSF9 protein
  

  Met	10-His tag	GGGSGGGSGGGSIEGR	Mouse 4-1BB Ligand (Arg104-Glu309) Accession # P41274
  N-terminus			C-terminus

• Accession #: P41274
• N-terminal Sequence: Met
• Protein/Peptide Type: Recombinant Proteins
• Gene: Tnfsf9
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
  • Bioactivity
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 41-44 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

• 41BB Ligand
• 4-1BB Ligand
• 4-1BBL
• 4-1BB-L
• CD137L
• homolog of mouse 4-1BB-L
• receptor 4-1BB ligand
• TNFSF9
• tumor necrosis factor (ligand) superfamily, member 9
• tumor necrosis factor ligand superfamily member 9

Background

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The approximately 50 kDa mouse 4-1BB Ligand consists of an 82 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, mouse 4-1BB Ligand shares 36% and 86% aa sequence identity with human and rat 4-1BB Ligand, respectively. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10). 4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4⁺ and CD8⁺ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4⁺ and CD8⁺ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through
4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
2. Goodwin, R.G. et al. (1993) Eur. J. Immunol. 23:2631.
3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
7. Baessler, T. et al. (2010) Blood 115:3058.
8. Gullo, C. et al. (2010) PLoS One 5:e10845.
9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
11. Wen, T. et al. (2002) J. Immunol. 168:4897.
12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Publications for 4-1BB Ligand/TNFSF9 (1246-4L)(5)

Publications using 1246-4L

• D Wan, S Ai, H Ouyang, L Cheng Activation of 4-1BB signaling in bone marrow stromal cells triggers bone loss via the p-38 MAPK-DKK1 axis in aged mice Experimental & Molecular Medicine, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859350] (Bioassay, Mouse)
• Madireddi S, Eun S, Lee S, Nemcovicova I, Mehta A, Zajonc D, Nishi N, Niki T, Hirashima M, Croft M Galectin-9 controls the therapeutic activity of 4-1BB-targeting antibodies. J Exp Med, 2014-06-23;211(7):1433-48. 2014-06-23 [PMID: 24958847] (Bioassay, Mouse)
• Mahmud , Shawn A, Manlove , Luke S, Schmitz , Heather, Xing , Yan, Wang , Yanyan, Owen , David L, Schenkel , Jason M, Boomer , Jonathan, Green , Jonathan, Yagita , Hideo, Chi , Hongbo, Hogquist , Kristin, Farrar , Michael Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells. Nat Immunol, 2014-03-16;15(5):473-81. 2014-03-16 [PMID: 24633226] (Bioassay, Mouse)
• Regulation of innate CD8+ T-cell activation mediated by cytokines. Proc Natl Acad Sci U S A, 2012-06-04;109(25):9971-6. 2012-06-04 [PMID: 22665806] (Bioassay, Mouse)
• Kayamuro H, Yoshioka Y, Abe Y, Katayama K, Yoshida T, Yamashita K, Yoshikawa T, Hiroi T, Itoh N, Kawai Y, Mayumi T, Kamada H, Tsunoda S, Tsutsumi Y TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 2009-05-04;384(3):296-300. 2009-05-04 [PMID: 19406102] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: Tnfsf9
• Uniprot:
  • P41274()