Recombinant Human Transglutaminase 2/TGM2 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Enzyme Activity

Order Details

Recombinant Human Transglutaminase 2/TGM2 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH. The specific activity is >1000 pmol/min/ug, as measured under the described condition.
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 2/TGM2 protein
Ala2-Ala687 (Asn533Thr and Leu655Val), with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
No results obtained
Protein/Peptide Type
Recombinant Enzymes
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
82 kDa, reducing conditions
Read Publications using
4376-TG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Diluent: deionized water
  • Recombinant Human Transglutaminase 2/TGM2 (rhTGM2) (Catalog # 4376-TG)
  • Substrate: Z-Gln-Gly (Sigma, Catalog # C6154), dissolve 500 mM in deionized water, then neutralize it to pH 9.0 with NaOH
  • MES, pH 6.0, 1 M stock
  • DTT (Sigma, Catalog # D-0632), 1 M stock in deionized water
  • CaCl2,1 M stock in deionized water
  • 1 M Hydroxylamine Hydrochloride (Sigma, Catalog # 159417), dissolve in deionized H2O, then neutralize it to pH 6.0 with NaOH
  • Stop Solution: 0.37 M FeCl3 (Sigma, Catalog # 236489), 0.67 M HCl, 0.2 M Trichloroacetic Acid
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: Spectramax Plus by Molecular Devices) or equivalent
  1. Prepare Substrate Mixture fresh before running assay. Mix the following components (Dilute all components to the correct concentration in Assay Diluent.):
    1. 10 µL of 500 mM Substrate
    2. 50 µL of 400 mM MES, pH 6.0
    3. 5 µL of 200 mM DTT
    4. 5 µL of 200 mM CaCl2
    5. 10 µL of 1 M Hydroxylamine Hydrochloride
      (Note: Multiply the volume for each component by the number of reaction vials + 1 to make enough substrate mixture for the assays. For example: If there are 2 reaction vials (including blank), multiply all volumes by 3.)
  2. Dilute rhTGM2 to 0.1 mg/mL in Assay Diluent.
  3. Mix 20 µL of the diluted rhTGM2 with 80 µL Substrate Mixture. For the Substrate Blank mix 20 µL of Assay Diluent with 80 µL Substrate Mixture.
  4. Incubate at 37 °C for 2 hours.
  5. After incubation, stop the reaction with 400 µL of the Stop Solution. Mix well.
  6. Centrifuge at top speed for 2 minutes in a microcentrifuge.
  7. Load 200 µL of the supernatant into a plate.
  8. Read at 525 nm (absorbance) in endpoint mode.
  9. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard L-glutamic acid gamma -monohydroxamate (Sigma, Catalog # G2253).

Per Well:
  • rhTGM2: 0.8 µg
  • Substrate: 10 mM


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Transglutaminase 2/TGM2 Protein, CF

  • C polypeptide
  • EC
  • G-ALPHA-h
  • protein-glutamine gamma-glutamyltransferase 2
  • protein-glutamine-gamma-glutamyltransferase
  • TG(C)
  • TG2
  • TGase C
  • TGase H
  • TGase-2
  • TGase-H
  • TGC
  • TGM2
  • Tissue transglutaminase
  • transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 2
  • Transglutaminase C
  • Transglutaminase H
  • Transglutaminase-2
  • tTG


Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein‑glutamine‑ gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).

  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

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Publications for Transglutaminase 2/TGM2 (4376-TG)(6)

We have publications tested in 2 confirmed species: Human, Plant - Wheat.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.

Filter By Application
Enzyme Assay
All Applications
Filter By Species
Plant - Wheat
All Species
Showing Publications 1 - 6 of 6.
Publications using 4376-TG Applications Species
LA Farrelly, RE Thompson, S Zhao, AE Lepack, Y Lyu, NV Bhanu, B Zhang, YE Loh, A Ramakrishn, KC Vadodaria, KJ Heard, G Erikson, T Nakadai, RM Bastle, BJ Lukasak, H Zebroski, N Alenina, M Bader, O Berton, RG Roeder, H Molina, FH Gage, L Shen, BA Garcia, H Li, TW Muir, I Maze Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3 Nature, 2019;0(0):. 2019 [PMID: 30867594] (Bioassay, Human) Bioassay Human
E Png, A Hou, L Tong Mechanistic role of transglutaminase-2 in focal adhesions Sci Rep, 2018;8(1):12370. 2018 [PMID: 30120307] (Enzyme Assay, Human) Enzyme Assay Human
C Valero, H Amaveda, M Mora, JM García-Azn Combined experimental and computational characterization of crosslinked collagen-based hydrogels PLoS ONE, 2018;13(4):e0195820. 2018 [PMID: 29664953] (Bioassay) Bioassay
Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease Sci Rep, 2016;6(0):30980. 2016 [PMID: 27481162] (Bioassay, Plant - Wheat) Bioassay Plant - Wheat
Enzymatically Regulated Peptide Pairing and Catalysis for the Bioanalysis of Extracellular Prometastatic Activities of Functionally Linked Enzymes Sci Rep, 2016;6(0):25362. 2016 [PMID: 27140831] (Enzyme Assay, Human) Enzyme Assay Human
Arjomandi M, Frelinger J, Donde A Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions. PLoS ONE, 2011;6(10):e25678. 2011 [PMID: 22031818] (Bioassay, Human) Bioassay Human

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Gene Symbol TGM2