Recombinant Human Transglutaminase 2/TGM2 Protein, CF

Images

 
There are currently no images for Transglutaminase 2/TGM2 (4376-TG).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human Transglutaminase 2/TGM2 Protein, CF Summary

Details of Functionality
Measured by its ability to cleave a synthetic peptide Benzyloxycarbonyl-Gln-Gly and NH2OH. The specific activity is >1000 pmol/min/ug, as measured under the described condition.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Transglutaminase 2/TGM2 protein
Ala2-Ala687 (Asn533Thr and Leu655Val), with an N-terminal Met and 6-His tag
Accession #
N-terminal Sequence
No results obtained
Protein/Peptide Type
Recombinant Enzymes
Gene
TGM2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
82 kDa, reducing conditions
Publications
Read Publications using
4376-TG in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer
Supplied as a 0.2 μm filtered solution in Tris, NaCl, Glycerol and DTT.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Assay Procedure
  • Assay Diluent: deionized water
  • Recombinant Human Transglutaminase 2/TGM2 (rhTGM2) (Catalog # 4376-TG)
  • Substrate: Z-Gln-Gly (Sigma, Catalog # C6154), dissolve 500 mM in deionized water, then neutralize it to pH 9.0 with NaOH
  • MES, pH 6.0, 1 M stock
  • DTT (Sigma, Catalog # D-0632), 1 M stock in deionized water
  • CaCl2,1 M stock in deionized water
  • 1 M Hydroxylamine Hydrochloride (Sigma, Catalog # 159417), dissolve in deionized H2O, then neutralize it to pH 6.0 with NaOH
  • Stop Solution: 0.37 M FeCl3 (Sigma, Catalog # 236489), 0.67 M HCl, 0.2 M Trichloroacetic Acid
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: Spectramax Plus by Molecular Devices) or equivalent
  1. Prepare Substrate Mixture fresh before running assay. Mix the following components (Dilute all components to the correct concentration in Assay Diluent.):
    1. 10 µL of 500 mM Substrate
    2. 50 µL of 400 mM MES, pH 6.0
    3. 5 µL of 200 mM DTT
    4. 5 µL of 200 mM CaCl2
    5. 10 µL of 1 M Hydroxylamine Hydrochloride
      (Note: Multiply the volume for each component by the number of reaction vials + 1 to make enough substrate mixture for the assays. For example: If there are 2 reaction vials (including blank), multiply all volumes by 3.)
  2. Dilute rhTGM2 to 0.1 mg/mL in Assay Diluent.
  3. Mix 20 µL of the diluted rhTGM2 with 80 µL Substrate Mixture. For the Substrate Blank mix 20 µL of Assay Diluent with 80 µL Substrate Mixture.
  4. Incubate at 37 °C for 2 hours.
  5. After incubation, stop the reaction with 400 µL of the Stop Solution. Mix well.
  6. Centrifuge at top speed for 2 minutes in a microcentrifuge.
  7. Load 200 µL of the supernatant into a plate.
  8. Read at 525 nm (absorbance) in endpoint mode.
  9. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard L-glutamic acid gamma -monohydroxamate (Sigma, Catalog # G2253).

Per Well:
  • rhTGM2: 0.8 µg
  • Substrate: 10 mM

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Transglutaminase 2/TGM2 Protein, CF

  • C polypeptide
  • EC 2.3.2.13
  • G-ALPHA-h
  • protein-glutamine gamma-glutamyltransferase 2
  • protein-glutamine-gamma-glutamyltransferase
  • TG(C)
  • TG2
  • TGase C
  • TGase H
  • TGase-2
  • TGase-H
  • TGC
  • TGCGNAH
  • TGM2
  • Tissue transglutaminase
  • transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
  • Transglutaminase 2
  • Transglutaminase C
  • Transglutaminase H
  • Transglutaminase-2
  • tTG

Background

Transglutaminase 2 (TG2), encoded by the TGM2 gene, is also known as tissue transglutaminase (tTG), transgluytaminase C (TGC), and protein‑glutamine‑ gamma -glutamyltransferase. It belongs to the family of transglutaminases that catalyze the posttranslational modification of proteins via calcium dependent cross-linking reactions (1‑3). In addition to its function in protein cross-linking, TGM2 is also capable of hydrolyzing both GTP and ATP (4) and has intrinsic kinase activity (5). TGM2 has been implicated in a variety of human diseases including celiac disease, inclusion body myositis, atherosclerosis, and neurodegenerative diseases (6, 7).

  1. Gentile, V. et al. (1991) J. Biol. Chem. 266:478.
  2. Chen, J.S.K. and K. Mehta (1999) Internat. J. Biochem. Cell Biol. 31:817.
  3. Griffin, M. et al. (2002) Biochem. J. 368:377.
  4. Lai, T.S. et al. (1998) J. Biol. Chem. 273:1776.
  5. Mishra, S. et al. (2007) J. Biol. Chem. 282:18108.
  6. Kim, S-Y. et al. (2002) Neurochem. Int. 40:85.
  7. Lesort, M. et al. (2000) Prog. Neurobiol. 61:439.

Customers Who Viewed This Item Also Viewed...

NBP2-70696
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, CyTOF-ready
NBP1-91258
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq, Fe
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-34062
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NB120-22711
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-16382
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP3-03780
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
NBP2-75531
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB100-56098
Species: Hu, Mu, Rt, Ca
Applications: WB, IHC, IHC-P, IP
AF808
Species: Mu
Applications: WB, IHC, ELISA(Cap), ELISA(Det), ICC, Neut, ELISA(Sta)
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB110-41551
Species: Hu, Mu, Rt, Ch
Applications: WB, IHC, IHC-Fr, IHC-P, IP
4376-TG
Species: Hu
Applications: Enzyme Activity

Publications for Transglutaminase 2/TGM2 (4376-TG)(6)

We have publications tested in 2 confirmed species: Human, Plant - Wheat.

We have publications tested in 2 applications: Bioassay, Enzyme Assay.


Filter By Application
Bioassay
(4)
Enzyme Assay
(2)
All Applications
Filter By Species
Human
(4)
Plant - Wheat
(1)
All Species
Showing Publications 1 - 6 of 6.
Publications using 4376-TG Applications Species
LA Farrelly, RE Thompson, S Zhao, AE Lepack, Y Lyu, NV Bhanu, B Zhang, YE Loh, A Ramakrishn, KC Vadodaria, KJ Heard, G Erikson, T Nakadai, RM Bastle, BJ Lukasak, H Zebroski, N Alenina, M Bader, O Berton, RG Roeder, H Molina, FH Gage, L Shen, BA Garcia, H Li, TW Muir, I Maze Histone serotonylation is a permissive modification that enhances TFIID binding to H3K4me3 Nature, 2019;0(0):. 2019 [PMID: 30867594] (Bioassay, Human) Bioassay Human
E Png, A Hou, L Tong Mechanistic role of transglutaminase-2 in focal adhesions Sci Rep, 2018;8(1):12370. 2018 [PMID: 30120307] (Enzyme Assay, Human) Enzyme Assay Human
C Valero, H Amaveda, M Mora, JM García-Azn Combined experimental and computational characterization of crosslinked collagen-based hydrogels PLoS ONE, 2018;13(4):e0195820. 2018 [PMID: 29664953] (Bioassay) Bioassay
Addressing proteolytic efficiency in enzymatic degradation therapy for celiac disease Sci Rep, 2016;6(0):30980. 2016 [PMID: 27481162] (Bioassay, Plant - Wheat) Bioassay Plant - Wheat
Enzymatically Regulated Peptide Pairing and Catalysis for the Bioanalysis of Extracellular Prometastatic Activities of Functionally Linked Enzymes Sci Rep, 2016;6(0):25362. 2016 [PMID: 27140831] (Enzyme Assay, Human) Enzyme Assay Human
Arjomandi M, Frelinger J, Donde A Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions. PLoS ONE, 2011;6(10):e25678. 2011 [PMID: 22031818] (Bioassay, Human) Bioassay Human

Reviews for Transglutaminase 2/TGM2 (4376-TG) (0)

There are no reviews for Transglutaminase 2/TGM2 (4376-TG). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Transglutaminase 2/TGM2 (4376-TG) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional Transglutaminase 2/TGM2 Products

Bioinformatics Tool for Transglutaminase 2/TGM2 (4376-TG)

Discover related pathways, diseases and genes to Transglutaminase 2/TGM2 (4376-TG). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Transglutaminase 2/TGM2 (4376-TG)

Discover more about diseases related to Transglutaminase 2/TGM2 (4376-TG).
 

Pathways for Transglutaminase 2/TGM2 (4376-TG)

View related products by pathway.

PTMs for Transglutaminase 2/TGM2 (4376-TG)

Learn more about PTMs related to Transglutaminase 2/TGM2 (4376-TG).

Blogs on Transglutaminase 2/TGM2

There are no specific blogs for Transglutaminase 2/TGM2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human Transglutaminase 2/TGM2 Protein, CF and receive a gift card or discount.

Bioinformatics

Gene Symbol TGM2
Uniprot