Recombinant Human TNF RII/TNFRSF1B Fc Avi-tag Protein, CF

2 μg/lane of Biotinylated Recombinant Human TNF RII/TNFRSF1B Fc Chimera Avi-tag Protein (Catalog # AVI10911) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 68-81 kDa and 140-160 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human TNF RII/TNFRSF1B Fc Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human TNF alpha (Catalog # 210-TA) is coated at 0.100 μg/mL (100 μL/well), Biotinylated Recombinant Human TNF RII/TNFRSF1B Fc Chimra Avi‑tag binds with an ED₅₀ of 4.00-24.0 ng/mL. The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.
• Source: Human embryonic kidney cell, HEK293-derived human TNF RII/TNFRSF1B protein
  

  Human TNF RII/TNFRSF1B (Leu23-Asp257) Accession #AAA36755.1	IEGRMD	Human IgG1 (Pro100-Lys330)	Avi-tag
  N-terminus			C-terminus

• Accession #: AAA36755.1
• N-terminal Sequence: Leu23
• Structure / Form: Biotinylated via Avi-tag.
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 68-81 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TNF RII/TNFRSF1B Fc Avi-tag Protein, CF

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily (1-4). The TNF receptor superfamily is comprised of structurally related receptors that bind to TNF-related ligands and regulate numerous processes such as immune cell activation and apoptosis. Receptors in this superfamily are characterized by the presence of a cysteine-rich region in their extracellular domain (ECD) (1-3, 5). Human TNF RII shares 57% and 56% sequence identity in the ECD with its mouse and rat homolog. Several receptors in the TNF superfamily also contain intracellular death domains (DDs) that recruit caspase-interacting proteins to initiate apoptosis upon ligand binding. Those receptors that lack DDs, like TNF RII, bind TNF Receptor-associated Factors, which transduce signals generated by activation of these receptors (6, 7). TNF RII is expressed predominantly on cells of the hematopoietic lineage, such as T and natural killer cells, as well as on endothelial cells, microglia, astrocytes, neurons, oligodendrocytes, cardiac myocytes, and thymocytes (6, 8, 9). TNF RII activation primarily initiates pro-inflammatory and pro-survival responses via NF kappa B-dependent signaling pathways (6, 7). However, under certain conditions, TNF RII signaling can induce apoptosis (8). Soluble TNF RII is believed to inhibit TNF biological activity by binding TNF thereby preventing it from activating membrane TNF receptors (10).. Our Avi-tag Biotinylated human TNF RII/TNFRSF1B Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

1. Dembic, Z. et al. (1990) Cytokine 2:231.
2. Kohno, T. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8331.
3. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
4. Loetscher, H. et al. (1990) J. Biol. Chem. 265:20131.
5. Beltinger, C.P. et al. (1996) Genomics 35:94.
6. Faustman, D. and M. Davis (2010) Nat. Rev. Drug Discov. 9:482.
7. Ihnatko, R. and M. Kubeš (2007) Gen. Physiol. Biophys. 26:159.
8. Mason, A.T. et al. (1995) J. Leukoc. Biol. 58:249.
9. Speeckaert, M.M. et al. (2012) Am. J. Nephrol. 36:261.
10. Sennikov, S.V. et al. (2014) Mediators Inflamm. 2014:745909.

Bioinformatics

• Uniprot:
  • AAA36755.1()