Recombinant Human Syndecan-3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Syndecan-3 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to inhibit the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. The ED₅₀ for this effect is 0.75-4.5 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Syndecan-3 protein
  Gln48-Lys383, with a C-terminal 6-His tag
• Accession #: NP_055469
• N-terminal Sequence: No results obtained: Gln48 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: SDC3
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 35.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 100-150 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Syndecan-3 Protein, CF

• KIAA0468
• N-Syndecan
• SDC3
• SDCN
• SYND3syndecan proteoglycan 3
• syndecan 3 (N-syndecan)
• syndecan 3
• syndecan neural type
• Syndecan3
• Syndecan-3

Background

Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of human Syndecan-3 shares 83%, 83%, 92%, 91% and 91% aa identity with of mouse, rat, equine, bovine and canine Syndecan-3, respectively. Splice isoforms of 384 aa and 346 aa, containing either a 28 aa substitution for aa 1 - 86 or deletion of aa 1 - 96, have been reported (4). Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N-terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 - 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta , it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 - 13). In adults, it is upregulated during regeneration, such as following myocardial infarction (14).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
2. Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
3. Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
4. ENTREZ protein Accession # O75056, EAX076736 and EAX07637.
5. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
6. Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
7. Hienola, A. et al. (2006) J. Cell Biol. 174:569.
8. Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
9. Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
10. Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
11. Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
12. Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
13. Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
14. Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.

Publications for SDC3 (3539-SD)(2)

Publications using 3539-SD

• Matsunuma, M;Kan, R;Yamada, Y;Hamada, K;Kanagawa, M;Nomizu, M;Kikkawa, Y; Chain-specificity of laminin ?1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding Scientific reports 2023-06-27 [PMID: 37369727] (Bioassay, Human)
• AD Eustace, EF McNaughton, S King, O Kehoe, A Kungl, D Mattey, AH Nobbs, N Williams, J Middleton Soluble syndecan-3 binds chemokines, reduces leukocyte migration in vitro and ameliorates disease severity in models of rheumatoid arthritis Arthritis Res. Ther., 2019-07-12;21(1):172. 2019-07-12 [PMID: 31300004] (In Vivo, Mouse)

Bioinformatics

• Gene Symbol: SDC3
• Uniprot:
  • NP_055469(Human)