Recombinant Human Syndecan-1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human Syndecan-1 Protein, CF Summary

Details of Functionality
Measured by the ability of the immobilized protein to enhance the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin.

When 5 x 104 cells/well are added to human Syndecan-1 and human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 2.5‑10 μg/mL.

Source
Mouse myeloma cell line, NS0-derived human Syndecan-1/CD138 protein
Gln18-Glu251, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
No results obtained: Gln18 predicted
Protein/Peptide Type
Recombinant Proteins
Gene
SDC1
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
60-90 kDa, reducing conditions
Publications
Read Publication using
2780-SD in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Syndecan-1 Protein, CF

  • CD138 antigen
  • CD138
  • SDC
  • SDC1
  • SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
  • syndecan 1
  • syndecan proteoglycan 1
  • syndecan
  • Syndecan1
  • Syndecan-1

Background

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65 - 71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4 - 8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10 - 12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).

  1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
  2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
  3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
  4. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
  5. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
  6. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
  7. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
  8. Vanhoutte, D. et al. (2007) Circulation 115:475.
  9. Li, Q. et al. (2002) Cell 111:635.
  10. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
  11. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
  12. Derksen, P.W.B. et al. (2002) Blood 99:1405.
  13. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

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Publications for Syndecan-1/CD138 (2780-SD)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol SDC1
Entrez
Uniprot