Recombinant Human Syndecan-1 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Syndecan-1 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to enhance the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin. Measured by the ability of the immobilized protein to enhance the adhesion of Saos‑2 human osteosarcoma cells to human Fibronectin (0.5 μg/mL, Catalog # 4305-FNB). The ED₅₀ for this effect is 2.5‑10 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Syndecan-1/CD138 protein
  Gln18-Glu251, with a C-terminal 6-His tag
• Accession #: NP_002988
• N-terminal Sequence: No results obtained: Gln18 predicted
• Protein/Peptide Type: Recombinant Proteins
• Gene: SDC1
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 24.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Syndecan-1 Protein, CF

• CD138 antigen
• CD138
• SDC
• SDC1
• SYND1heparan sulfate proteoglycan fibroblast growth factor receptor
• syndecan 1
• syndecan proteoglycan 1
• syndecan
• Syndecan1
• Syndecan-1

Background

Syndecan-1, designated CD138, is a dimeric type I transmembrane (TM) protein that belongs to the syndecan family of Type 1 transmembrane proteins (1, 2). The four syndecan family members are major carriers of heparan sulfate (HS) and chondroitin sulfate glycosaminoglycans (GAGs) that have different expression patterns and extracellular sequences. Syndecan-1 forms weak non-covalent homodimers, or heterodimers with Syndecan-2 or -3, through interactions of the transmembrane domain (3). It is synthesized as a 310 amino acid (aa) precursor with a 17 aa signal sequence, a 234 aa extracellular domain (ECD) that includes three closely-spaced consensus Ser-Gly HS attachment sites near the N-terminus, a 25 aa TM segment, and a 34 aa cytoplasmic region that includes a PDZ binding motif with a tyrosine phosphorylation site. The ECD is variably modified by GAGs, producing molecular weights of 120 - 200 kDa for native Syndecan-1. Soluble forms are shed via proteolytic cleavage. Human Syndecan-1 ECD shares 65 - 71% aa identity with the ECD of rat, mouse, canine, equine and bovine Syndecan-1. Syndecan-1 shows highest expression on epithelial cells such as keratinocytes, and terminally differentiated B cells such as plasma cells (4, 5). It aids wound healing in skin, cornea, and heart following myocardial infarction by promoting re-epithelialization, migration, and collagen deposition (4 - 8). It binds chemokines, creating chemotactic gradients when shed, but also binds and modulates integrins to control the influx of leukocytes (5, 7, 9). The net effect is to allow, but limit, inflammation. In myeloma and other cancers, shedding of Syndecan-1 can facilitate growth, angiogenesis and metastasis (10 - 12). Growth factors, such as FGFs and HGF, bind GAG chains and use Syndecan-1 as a coreceptor (12, 13). The GAG chains may also be used by a variety of viruses and bacteria for cell adhesion and uptake (4).

1. Tkachenko, E. et al. (2005) Circ. Res. 96:488.
2. Mali, M. et al. (1990) J. Biol. Chem. 265:6884.
3. Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
4. Fears, C.Y. and A. Woods (2006) Matrix Biol. 25:443.
5. Stepp, M.A. et al. (2002) J. Cell Sci. 115:4517.
6. Ojeh, N. et al. (2008) J. Invest. Dermatol. 128:26.
7. Stepp, M.A. et al. (2007) J. Cell Sci. 120:2851.
8. Vanhoutte, D. et al. (2007) Circulation 115:475.
9. Li, Q. et al. (2002) Cell 111:635.
10. Beauvais, D.M. et al. (2009) J. Exp. Med. 206:691.
11. Yang, Y. et al. (2007) J. Biol. Chem. 282:13326.
12. Derksen, P.W.B. et al. (2002) Blood 99:1405.
13. Su, G. et al. (2007) J. Biol. Chem. 282:14906.

Publications for Syndecan-1/CD138 (2780-SD)(5)

Publications using 2780-SD

• Matsunuma, M;Kan, R;Yamada, Y;Hamada, K;Kanagawa, M;Nomizu, M;Kikkawa, Y; Chain-specificity of laminin ?1-5 LG45 modules in the recognition of carbohydrate-linked receptors and intramolecular binding Scientific reports 2023-06-27 [PMID: 37369727] (Bioassay, Human)
• MW Britten, L Lümers, K Tominaga, J Peters, D Dirkmann Glycocalyx components affect platelet function, whole blood coagulation, and fibrinolysis: an in vitro study suggesting a link to trauma-induced coagulopathy BMC anesthesiology, 2021-03-19;21(1):83. 2021-03-19 [PMID: 33740916] (Human)
• AF Asega, MC Menezes, D Trevisan-S, D Cajado-Car, L Bertholim, AK Oliveira, A Zelanis, SMT Serrano Cleavage of proteoglycans, plasma proteins and the platelet-derived growth factor receptor in the hemorrhagic process induced by snake venom metalloproteinases Sci Rep, 2020-07-31;10(1):12912. 2020-07-31 [PMID: 32737331] (Bioassay, Snake)
• JI Buchheim, MC Enzinger, A Choukèr, M Bruegel, L Holdt, M Rehm The stressed vascular barrier and coagulation - The impact of key glycocalyx components on in vitro clot formation Thromb. Res., 2019-12-28;186(0):93-102. 2019-12-28 [PMID: 31927395] (Activation, Human)
• Dengue Virus NS1 Disrupts the Endothelial Glycocalyx, Leading to Hyperpermeability PLoS Pathog, 2016-07-14;12(7):e1005738. 2016-07-14 [PMID: 27416066] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SDC1
• Uniprot:
  • NP_002988()