Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

Recombinant Human Stanniocalcin 2/STC-2 (Catalog # 9405-SO) inhibits Recombinant Mouse Wnt-3a induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 60-360 ng/ml in the presence of 10 ng/ml of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Stanniocalcin 2/STC-2 Protein, CF Summary

• Details of Functionality: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 60-360 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
• Source: Mouse myeloma cell line, NS0-derived human Stanniocalcin 2/STC-2 protein
  Thr25-Arg302, with a C-terminal 6-His tag
• Accession #: O76061
• N-terminal Sequence: Thr25
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 34-43 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Stanniocalcin 2/STC-2 Protein, CF

• Stanniocalcin 2
• stanniocalcin-2
• STC2
• STC-2
• STC-2Stanniocalcin-related protein
• STC-related protein
• STCRP

Background

Stanniocalcin 2 (STC-2) is a secreted disulfide-linked homodimeric glycoprotein hormone that is related to the STC protein first discovered from corpuscles of stannius in fish (1). The STC-2 has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues (2). Its C-terminus contains a cluster of histidine residues which may interact with metal ions (1). STC-2 is expressed in a wide variety of tissues. In the ovary, STC-2 has been shown to be a paracrine hormone that regulates granulosa cell function (1). The amino acid sequence of human mature STC-2 is 36% identical to that of human STC-1. It is also 99% and 88% identical to that of monkey and mouse STC-2, respectively (3). STC-2 enhances mesenchymal stem cell survival (4), promotes cell proliferation in cervical cancer (5), suppresses breast cancer cell migration and invasion (6), promotes osteoblast differentiation (7), and inhibits longitudinal bone growth directly at the growth plate (8). It is also a biomarker for cervical and lung cancers (9, 10),

1. Luo, C. et al. (2005). Endocrinology 146:469.
2. Tagliabracci, V. S. et al. (2015). Cell 161:1619.
3. Ishibashi, K. et al. (1998). Biochem. Biophys. Res. Commun. 250:252.
4. Kim PH, et al. (2015) BMB Rep. 48:702.
5. Wang Y, et al. (2015) Biochem Biophys Res Commun. 466:362.
6. Hou J, et al. (2015) PLoS One. 10:e0122179.
7. Zhou J, et al. (2016) Mol Med Rep. 14:5653.
8. Wu S, et al. (2006) J Biol Chem. 281: 5120.
9. Shen XJ, et al. (2014) Int J Clin Exp Pathol. 7: 8770.
10. Na SS, et al. (2015) Biochim Biophys Acta. 1854:668.

Bioinformatics

• Uniprot:
  • O76061()