Recombinant Human SCUBE3 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human SCUBE3 Protein, CF Summary

• Details of Functionality: Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is typically 0.25-1.25 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human SCUBE3 protein
  Ala21-Lys993, with an N-terminal HA-tag (YPYDVPDYA)
• Accession #: Q8IX30
• N-terminal Sequence: Tyr & Arg538
• Protein/Peptide Type: Recombinant Proteins
• Gene: SCUBE3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 108.3 kDa (Monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 110-130 kDa & 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCUBE3 Protein, CF

• CEGF3
• CUB and EGF containing protein 3
• CUB domain and EGF-like repeat containing 3
• DKFZp686B09105
• DKFZp686B1223
• DKFZp686D20108
• FLJ34743
• SCUBE3
• signal peptide, CUB and EGF-like domain containing protein 3
• signal peptide, CUB and EGF-like domain-containing protein 3
• signal peptide, CUB domain, EGF-like 3

Background

SCUBE3 (signal peptide, CUB and EGF-like domain-containing protein 3) is a member of the SCUBE family of secreted glycoproteins (1). Like other SCUBE proteins, the 993 amino acid (aa) human SCUBE3 precursor contains a signal peptide (aa 1‑20), nine EGF‑like motifs (aa 29‑398), a linker region with multiple N‑glycosylation sites (aa 399‑803) and a C‑terminal CUB domain (aa 804‑916). Cysteines within the EGF‑like and linker regions are mainly conserved (1). The 130 kDa, mature, full‑length form of human SCUBE3 shares 96%, 93%, 96%, 95%, 95% and 94% aa sequence identity with mouse, rat, equine, bovine, canine and porcine SCUBE3, respectively (1). It also shares 66% and 58% aa identity with human SCUBE1 and SCUBE2, respectively. Cleavage by proteases such as MMP‑2 and MMP‑9 produces a CUB domain-containing, 55 kDa fragment that, like full‑length SCUBE3, binds TGF-beta RII (1, 2). SCUBE proteins form homo-oligomers and hetero‑oligomers, and are predicted to affect TGF‑ beta and Hedgehog signaling (2‑5). SCUBE3 is most highly expressed in osteoblasts (1, 3, 6). It is also found in umbilical vein endothelial cells with SCUBE1 and SCUBE2, and in cardiac ventricular myocytes (1, 3, 6). Transgenic over-expression of SCUBE3 can cause progressive cardiac hypertrophy (3). SCUBE3 and its active fragment are frequently over‑expressed in lung cancer tumor tissues, and can promote epithelial to mesenchymal transition by enhancing TGF-beta signaling pathways (2).

1. Wu, B.T. et al. (2004) J. Biol. Chem. 279:37485.
2. Wu, Y.Y. et al. (2011) Oncogene 30:3682.
3. Yang, H.Y. et al. (2007) Cardiovasc. Res. 75:139.
4. Johnson, J.L. et al. (2012) Dev. Biol. 368:193.
5. Creanga, A. et al. (2012) Genes Dev. 26:1312.
6. Haworth, K. et al. (2007) Gene Expr. Patterns 7:630.

Publications for SCUBE3 (7730-SC)(1)

Publication using 7730-SC

• Yingzi Liu, Christian F. Guerrero-Juarez, Fei Xiao, Nitish Udupi Shettigar, Raul Ramos, Chen-Hsiang Kuan et al. Hedgehog signaling reprograms hair follicle niche fibroblasts to a hyper-activated state Developmental Cell 2022-07-01 [PMID: 35777353] (In vivo assay, Mouse)

Bioinformatics

• Gene Symbol: SCUBE3
• Uniprot:
  • Q8IX30(Human)