Recombinant Human SCF Protein, CF

Images

 
1 μg/lane of Recombinant Human SCF/c-kit Ligand was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC/CF) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human SCF Protein, CF Summary

Additional Information
A New rh SCF is Now Available! It has a lower endotoxin specification and is human cell expressed!
Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 1‑5 ng/mL.
The specific activity of Recombinant Human SCF is approximately 1.3 x 106 IU/mg, which is calibrated against recombinant human SCF WHO International Standard (NIBSC code: 91/682).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human SCF/c-kit Ligand protein
Glu26-Ala189, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
KITLG
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
17-18 kDa, under reducing condtitions
Publications
Read Publications using
255-SC/CF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100-200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human SCF Protein, CF

  • c-kit Ligand
  • DCUA
  • DFNA69
  • DKFZp686F2250
  • familial progressive hyperpigmentation 2
  • FPH2
  • FPHH
  • KIT ligand
  • Kitl
  • KITLG
  • KL-1
  • Mast cell growth factor
  • MGF
  • MGFSHEP7
  • SCF
  • SCFStem cell factor
  • SFc-Kit ligand
  • SHEP7
  • SLF
  • steel factor

Background

What is Stem cell factor?

Stem cell factor (SCF) is a potent hematopoietic growth factor required in regulating both embryonic and adult hematopoiesis. SCF protein promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors (1 7). SCF is a primary growth and activation factor for mast cells and eosinophils (8). And SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels (9). Stem cell factor is an important cytokine for ex vivo clinical applications. Along with other cytokines, SCF is used in the culture and expansion of hematopoietic stem cells (HSCs) as well as for proliferation and differentiation of both myeloid and erythroid progenitor cells.

Mature stem cell factor consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane domain, and a 36 aa cytoplasmic tail (10). The ECD shows both N linked and O-linked glycosylation (11). SCF protein exists in two forms, a membrane-bound form and a proteolytically processed soluble form that lacks the transmembrane domain and cytoplasmic tail. The soluble form is created by proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releasing a 25 kDa soluble SCF protein which is comparable to the only form produced by Steel-dickie mutant mice (12, 13). There is also an alternately spliced isoform of human SCF that lacks 28 amino acids that encompasses the primary proteolytic recognition site (14). This form cannot be cleaved and is only membrane bound.

SCF binds to C-kit (CD117). C-kit is expressed on many different cell types including HSCs, mast cells, germ cells, and melanocytes. Binding of SCF to C-kit induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain (15). Tyrosine phosphorylation initiates multiple signaling pathways including RAS, PI3 kinase, Src, and JAK/STAT. Stem cell factor is highly conserved among mammals. Human SCF protein shares 79% 87% aa sequence identity with dog, cat, mouse, and rat SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells (10).

  1. Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31:1037. 
  2. Sette, C. et al. (2000) Int. J. Dev. Biol. 44:599. 
  3. Yoshida, H. et al. (2001) J. Invest. Dermatol. Symp. Proc. 6:1. 
  4. Erlandsson, A. et al. (2004) Exp. Cell Res. 301:201. 
  5. Kapur, R. et al. (2002) Blood 100:1287. 
  6. Wang, C.-H. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:540. 
  7. Bashamboo, A. et al. (2006) J. Cell Sci. 119:3039. 
  8. Reber, L. et al. (2006) Eur. J. Pharmacol. 533:327.
  9. Kanellakis, P. et al. (2006) Cardiovasc. Res. 70:117.
  10. Martin, F.H. et al. (1990) Cell 63:203.
  11. Arakawa, T. et al. (1991) J. Biol. Chem. 266:18942.
  12. Majumdar, M.K. et al. (1994) J. Biol. Chem. 269:1237.
  13. Brannan, C.I. et al. (1991) Proc. Natl. Acad. Sci. 88:4671.
  14. Anderson, D.M. et al. (1991) Cell Growth Differ. 2:373.
  15. Lemmon, M.A. et al. (1997) J. Biol. Chem. 272:6311.

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Publications for SCF/c-kit Ligand (255-SC/CF)(165)

We have publications tested in 8 confirmed species: Human, Mouse, Hamster, N/A, Porcine, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio anubis (Olive Baboon).

We have publications tested in 4 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard).


Filter By Application
Bioassay
(147)
Cell Culture
(14)
Differentiation
(1)
ELISA (Standard)
(1)
All Applications
Filter By Species
Human
(151)
Mouse
(2)
Hamster
(1)
N/A
(1)
Porcine
(1)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(5)
Primate - Papio anubis (Olive Baboon)
(1)
All Species
Showing Publications 1 - 10 of 165. Show All 165 Publications.
Publications using 255-SC/CF Applications Species
Y Liu, Y Liu, Y Jia, J Lv, D Sun, Y Yin, N Liu, B Su, G Sun Generation of an induced pluripotent stem cell (iPSC) line from a diabetic patient with glucagon receptor (GCGR) p.W83X mutation Stem Cell Research, 2022;61(0):102745. 2022 [PMID: 35381520] (Bioassay, Human) Bioassay Human
G Iannello, A Patel, D Sirabella, B Corneo, VV Thaker Derivation and characterization of the induced pluripotent stem cell line CUIMCi004-A from a patient with a novel frameshift variant in exon 18a of OCRL Stem Cell Research, 2021;59(0):102635. 2021 [PMID: 35074682] (Bioassay, Human) Bioassay Human
M Sone, S Nakamura, S Umeda, H Ginya, M Oshima, MA Kanashiro, SK Paul, K Hashimoto, E Nakamura, Y Harada, K Tsujimura, A Saraya, T Yamaguchi, N Sugimoto, A Sawaguchi, A Iwama, K Eto, N Takayama Silencing of p53 and CDKN1A establishes sustainable immortalized megakaryocyte progenitor cells from human iPSCs Stem Cell Reports, 2021;0(0):. 2021 [PMID: 34861163] (Bioassay, Human) Bioassay Human
AF de Souza, FF Bressan, NCG Pieri, RC Botigelli, T Revay, SK Haddad, DT Covas, ES Ramos, WA King, FV Meirelles Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients Cells, 2021;10(11):. 2021 [PMID: 34831322] (Bioassay, Human) Bioassay Human
SS Sahoo, VB Pastor, C Goodings, RK Voss, EJ Kozyra, A Szvetnik, P Noellke, M Dworzak, J Starý, F Locatelli, R Masetti, M Schmugge, B De Moerloo, A Catala, K Kállay, D Turkiewicz, H Hasle, J Buechner, K Jahnukaine, M Ussowicz, S Polychrono, OP Smith, O Fabri, S Barzilai, V de Haas, I Baumann, S Schwarz-Fu, European W, MR Niewisch, MG Sauer, B Burkhardt, P Lang, P Bader, R Beier, I Müller, MH Albert, R Meisel, A Schulz, G Cario, PK Panda, J Wehrle, S Hirabayash, M Derecka, R Durruthy-D, G Göhring, A Yoshimi-No, M Ku, D Lebrecht, M Erlacher, C Flotho, B Strahm, CM Niemeyer, MW Wlodarski Clinical evolution, genetic landscape and trajectories of clonal hematopoiesis in SAMD9/SAMD9L syndromes Nature Medicine, 2021;27(10):1806-1817. 2021 [PMID: 34621053] (Bioassay, Human) Bioassay Human
S Yu, C Di, S Chen, M Guo, J Yan, Z Zhu, L Liu, R Feng, Y Xie, R Zhang, J Chen, M Wang, D Wei, H Fang, T Yin, J Huang, S Chen, H Lu, J Zhu, J Qu Distinct immune signatures discriminate between asymptomatic and presymptomatic SARS-CoV-2pos subjects Cell Research, 2021;0(0):. 2021 [PMID: 34561618] (Bioassay, Human) Bioassay Human
GB Schwartz, H Kubo, MM Laronda Generation of two human induced pluripotent stem cell lines from a patient with complete androgen insensitivity syndrome with a hemizygous single nucleotide variant in the androgen receptor (AR) gene Stem Cell Research, 2021;55(0):102441. 2021 [PMID: 34233262] (Bioassay, Human) Bioassay Human
H Kato, Y Maezawa, Y Ouchi, N Takayama, M Sone, K Sone, A Takada-Wat, K Tsujimura, M Koshizaka, S Nagasawa, H Saitoh, M Ohtaka, M Nakanishi, H Tahara, A Shimamoto, A Iwama, K Eto, K Yokote Generation of disease-specific and CRISPR/Cas9-mediated gene-corrected iPS cells from a patient with adult progeria Werner syndrome Stem Cell Research, 2021;53(0):102360. 2021 [PMID: 34087989] (Cell Culture, Human) Cell Culture Human
GA Newby, JS Yen, KJ Woodard, T Mayuranath, CR Lazzarotto, Y Li, H Sheppard-T, SN Porter, Y Yao, K Mayberry, KA Everette, Y Jang, CJ Podracky, E Thaman, C Lechauve, A Sharma, JM Henderson, MF Richter, KT Zhao, SM Miller, T Wang, LW Koblan, AP McCaffrey, JF Tisdale, TA Kalfa, SM Pruett-Mil, SQ Tsai, MJ Weiss, DR Liu Base editing of haematopoietic stem cells rescues sickle cell disease in mice Nature, 2021;0(0):. 2021 [PMID: 34079130] (Bioassay, Human) Bioassay Human
JC Reid, D Golubeva, AL Boyd, CG Hollands, C Henly, L Orlando, A Leber, J Hébert, F Morabito, G Cutrona, L Agnelli, M Gentile, M Ferrarini, A Neri, B Leber, M Bhatia Human pluripotent stem cells identify molecular targets of trisomy 12 in chronic lymphocytic leukemia patients Cell Reports, 2021;34(11):108845. 2021 [PMID: 33730576] (Bioassay, Human) Bioassay Human
Show All 165 Publications.

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Bioinformatics

Gene Symbol KITLG
Uniprot