Recombinant Human Pentraxin 2/SAP Protein

Product Discontinued

Recombinant Human Pentraxin 2/SAP Protein Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Fc gamma RllA/CD32a (Catalog # 1330-CD) is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Human Pentraxin 2/SAP that produces 50% of the optimal binding response is 0.175-1 μg/mL.
• Source: Mouse myeloma cell line, NS0-derived human Pentraxin 2/SAP protein
  His20-Val223, with a C-terminal 10-His tag
• Accession #: P02743
• N-terminal Sequence: His20
• Structure / Form: Noncovalently-linked homopentamer
• Protein/Peptide Type: Recombinant Proteins
• Gene: APCS
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 25 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 28-30 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Pentraxin 2/SAP Protein

• 9.5S alpha-1-glycoprotein
• amyloid P component, serum
• APCS
• MGC88159
• Pentraxin 2
• PTX2
• PTX2serum amyloid P-component
• SAP
• SAPpentaxin-related
• Serum Amyloid P component

Background

Pentraxin 2 (PTX2), also known as Serum Amyoid P Component (SAP), is a secreted serum glycoprotein that is a universal non-fibrillar component of amyloid deposits. These extracellular deposits of insoluble protein fibrils are the result of protein misfolding and can lead to tissue damage and disease (1, 2). PTX2 belongs to the pentaxin superfamily, whose members have the characteristic pentagonal discoid arrangement of five non-covalently bound subunits. Pentaxins bind to a variety of molecules in a calcium-dependent lectin-like manner through a pattern-recognition-binding site (1, 4, 5). Two subfamilies of pentaxins, the classical or short pentaxin subfamily that includes the serum C-reactive protein (CRP) and PTX2, and the fusion or long pentaxin subfamily whose members contain pentaxin-related
carboxyl-terminal halves, are known (1).

PTX2 and CRP share approximately 50% amino acid sequence identity (2, 5). They are produced and secreted by liver hepatocytes and circulates in plasma. Mouse PTX2 is a major acute-phase protein whose plasma concentrations increase dramatically during an acute phase response (2). In human where CRP is the major
acute-phase protein, the plasma concentration of human PTX2 remains relatively constant in response to tissue-damage (2, 5). The gene for PTX2 has been localized to human chromosome 1 where it is closely linked to the gene for CRP.

PTX2 associates ubiquitously with all amyloid deposits that are implicated in a diverse range of diseases including Alzheimer’s and prion diseases, type 2 diabetes and various systemic amyloidoses (3, 6, 7). As a non-fibrillar component, PTX2 regulates the solubility of amyloid fibrils and protects them from degradation by proteolytic enzymes and phagocytic cells. In addition to its role in the pathogenesis of amyloidoses, PTX2 also has an important physiological function in innate immunity (8). It is an opsonin that interacts with all three types of human Fc gamma receptors that mediate phagocytosis by human polymorphonuclear leukocytes. It has been proposed that PTX2 may function as an opsonin for a variety of ligands including autoantigens, apoptotic cells, chromatin, DNA, and micro-organisms.

1. Goodman, A. et al. (1996) Cytokine Growth Factor Rev. 7:191.
2. Steel, D. and A. Whitehead (1994) Immunol. Today 15:81.
3. Hirschfield, G.M. and P.N. Hawkins (2003) Int. J. Biochem. Cell Biol. 35:1608.
4. Emsley, J. et al. (1994) Nature 367:338.
5. Mantzouranis, E. et al. (1985) J. Biol. Chem. 260:7752.
6. Botto, M. et al. (1997) Nature Medicine 3:855.
7. Pepys, M. et al. (2002) Nature 417:254.
8. Bharadwaj, D. et al. (2001) J. Immunol. 166:6735.

Publications for Pentraxin 2/SAP (1948-SA)(4)

Publications using 1948-SA

• L Wang, D Liang, Y Huang, Y Chen, X Yang, Z Huang, Y Jiang, H Su, L Wang, JL Pathak, L Ge SAP deficiency aggravates periodontitis possibly via C5a-C5aR signaling-mediated defective macrophage phagocytosis of Porphyromonas gingivalis Journal of advanced research, 2022-10-12;0(0):. 2022-10-12 [PMID: 36243399] (Bioassay, Human)
• Pentraxin-2 suppresses c-Jun/AP-1 signaling to inhibit progressive fibrotic disease JCI Insight, 2016-12-08;1(20):e87446. 2016-12-08 [PMID: 27942582] (Bioassay, Human)
• Nobutaka Wakamiya Three pentraxins C-reactive protein, serum amyloid p component and pentraxin 3 mediate complement activation using Collectin CL-P1 Biochim. Biophys. Acta, 2016-11-15;0(0):. 2016-11-15 [PMID: 27864148] (Bioassay)
• Haudek SB, Trial J, Xia Y, Gupta D, Pilling D, Entman ML Fc receptor engagement mediates differentiation of cardiac fibroblast precursor cells. Proc. Natl. Acad. Sci. U.S.A., 2008-07-15;105(29):10179-84. 2008-07-15 [PMID: 18632582] (Bioassay, Human)

Bioinformatics

• Gene Symbol: APCS
• Uniprot:
  • P02743()