Recombinant Human PDGFRL Fc Chimera Protein, CF

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Recombinant Human PDGFRL Fc Chimera (Catalog # 1723-PR) inhibits HCT-116 human Colorectal carcinoma proliferation. The ED50 for this effect is 1.5-9 μg/mL.
2 μg/lane of Recombinant Human PDGFRL Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 69- 84 kDa and 140- 170 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human PDGFRL Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HCT-116 human colorectal carcinoma cells. The ED50 for this effect is 1.5-9 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human PDGFRL protein
Human PDGFRL
(Gln22-Ser375)
Accession # Q15198
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Gln22 inferred from enzymatic pyroglutamate treatment revealing His23.
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
69-84 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGFRL Fc Chimera Protein, CF

  • PDGFRL
  • PDGFR-Like Protein
  • PDGRL
  • platelet-derived growth factor receptor-like protein
  • platelet-derived growth factor receptor-like
  • platelet-derived growth factor-beta-like tumor suppressor
  • PRLTS
  • PRLTSPDGF receptor beta-like tumor suppressor

Background

Platelet-derived growth factor receptor-like protein is a 67 kDa glycoprotein protein consisting of two Ig-like C2-type domains. By sequence similarity, human PDGFRL is 90% similar to the mouse version and 91% similar to that of the rat. It is a secreted protein related to the class III subfamily of receptor tyrosine kinases (RTK), the platelet-derived growth factor receptors (1-5). PDGFRL is a tumor suppressor active in the tumor-suppression network and implicated in colorectal cancer, and a decrease in PDGFRL expression levels has been observed in breast cancer (1). A variant of PDGFRL is found to play a role in the development of Behçet disease, a complex immunoregulatory disease (6). The autoimmune role of PDGFRL is also supported by its up-regulation in a mouse model for Rheumatoid Arthritis (7). Study also showed that PDGFRL may play a role in chondrocyte proliferation and differentiation.
  1. Guo, F-J. et al. (2010) World J Gastroenterol. 16:1465.
  2. Andrae, J. et al. (2008) Genes Dev. 22:1276.
  3. Heldin, C-H. and B. Westermark (1999) Physiol. Rev. 79:1283.
  4. Claesson-Welsh, L. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4917.
  5. Matsui, T. et al. (1989) Science 243:800.
  6. Hou, S. et al. (2013). Human Mutat. 34:74.
  7. Fujikado, N. et al. (2006). Arthritis Res. & Ther. 8:R100.
  8. Kawata, K. et al. (2017) J Cell Biochemistry. 118:4033.

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