Recombinant Human PDGF R beta Fc Chimera Avi-tag Protein, CF

When Recombinant Human PDGF-BB (220-BB) is coated at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human PDGF R beta Fc Chimera Avi-tag (Catalog # AVI10652) binds with an ED50 of 12-120 ng/mL.

2 μg/lane of Biotinylated Recombinant Human PDGF R beta Fc Chimera Avi-tag (Catalog # AVI10652) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 107-119 kDa and 200-230 kDa, respectively.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human PDGF R beta Fc Chimera Avi-tag Protein, CF Summary

• Additional Information: Biotinylated
• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human PDGF-BB (Catalog # 220-BB) is coated at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human PDGF R beta Fc Chimera Avi-tag (Catalog # AVI10652) binds with an ED 50 of 12-120 ng/mL. Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED 50 for this effect is 1-3 µg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human PDGF R beta protein

  Human PDGF R beta (Leu33-Lys531) Accession # AAA36427.1

  IEGRMD

  Human IgG1 (Pro100-Lys330)

  Avi-tag

  N-terminus

  C-terminus

• Accession #: AAA36427.1
• N-terminal Sequence: Leu33
• Structure / Form: Disulfide-linked homodimer, biotinylated via Avi-tag
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Bioactivity2
• Theoretical MW: 85 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 107-119 kDa, under reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human PDGF R beta Fc Chimera Avi-tag Protein, CF

• beta-type platelet-derived growth factor receptor
• CD140 antigen-like family member B
• CD140b antigen
• CD140b
• EC 2.7.10
• EC 2.7.10.1
• JTK12
• PDGF R beta
• PDGFR
• PDGFR1
• PDGFRB
• PDGF-R-beta
• platelet-derived growth factor receptor, beta polypeptide

Background

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist. Our Avi-tag Biotinylated Human PDGF R beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity

1. Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Bioinformatics

• Uniprot:
  • AAA36427.1()