Recombinant Human Neuroligin 3/NLGN3 Protein, CF

When Recombinant Human Neuroligin 3/NLGN3 (Catalog # 9069-NL) is immobilized at 2 µg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED50 of 150-900 ng/mL.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human Neuroligin 3/NLGN3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 3/NLGN3 is immobilized at 2 μg/mL, Biotinylated Recombinant Human Neurexin 1 beta Fc Chimera binds with a typical ED₅₀ = 150-900 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Neuroligin 3/NLGN3 protein
  Ala39-Ser709 (Ser92Tyr), with a C-terminal 6-His tag
• Accession #: Q9NZ94
• N-terminal Sequence: Ala39
• Protein/Peptide Type: Recombinant Proteins
• Gene: NLGN3
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 87-101 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Neuroligin 3/NLGN3 Protein, CF

• ASPGX1
• AUTSX1
• Gliotactin homolog
• HNL3
• KIAA1480HNL3
• Neuroligin 3
• neuroligin-3
• NL3
• NLGN3

Background

Neuroligin 3 (NLGN3) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1, 2). Mature human Neuroligin 3 is an approximately 120 kDa molecule that consists of a 672 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 118 aa cytoplasmic tail (3). Alternative splicing generates additional isoforms with 20 aa or 40 aa deletions in the ECD (3-5). Within the ECD, human Neuroligin 3 shares 99% aa sequence identity with comparable regions of mouse and rat Neuroligin 3. It is expressed during development on astrocytes and Schwann cells (6) as well as on neurons where it localizes to synaptic membranes and post-synaptic densities of both glutamatergic and GABAergic synapses (7). It associates in cis with Neuroligin 1 and Neuroligin 2 and trans-synaptically with Neurexin-1 beta , -2 beta , and -3 beta that lack the splice site 4 insertion (3, 7). A soluble form of the Neuroligin 3 ECD can be released by neurons and promotes glioma cell proliferation (8). Mutations in Neuroligin 3 enhance neuronal dendritic branching, alter both excitatory and inhibitory synaptic activity (9-12), and are associated with the development of autism spectrum disorder (13, 14).

1. Bemben, M.A. et al. (2015) Trends Neurosci. 38:496.
2. Mackowiak, M. et al. (2014) Pharmacol. Rep. 66:830.
3. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
4. Philibert, R.A. et al. (2000) Gene 246:303.
5. Talebizadeh, Z. et al. (2006) J. Med. Genet. 43:e21.
6. Gilbert, M. et al. (2001) Glia 34:151.
7. Budreck, E.C. and P. Scheiffele (2007) Eur. J. Neurosci. 26:1738.
8. Venkatesh, H.S. et al. (2015) Cell 161:803.
9. Etherton, M. et al. (2011) Proc. Natl. Acad. Sci. USA 108:13764.
10. Foldy, C. et al. (2013) Neuron 78:498.
11. Tabuchi, K. et al. (2007) Science 318:71.
12. Gutierrez, R.C. et al. (2009) Neuroscience 162:208.
13. Jamain, S. et al. (2003) Nat. Genet. 34:27.
14. Rothwell, P.E. et al. (2014) Cell 158:198.

Publications for Neuroligin 3/NLGN3 (9069-NL)(3)

Publications using 9069-NL

• E Camporesi, T Lashley, J Gobom, J Lantero-Ro, O Hansson, H Zetterberg, K Blennow, B Becker Neuroligin-1 in brain and CSF of neurodegenerative disorders: investigation for synaptic biomarkers Acta neuropathologica communications, 2021-02-01;9(1):19. 2021-02-01 [PMID: 33522967] (Reference Standard, Human)
• G Goranci-Bu, A Mariappan, E Gabriel, A Ramani, L Ricci-Viti, M Buccarelli, QG D'Alessand, R Pallini, J Gopalakris Rapid and Efficient Invasion Assay of Glioblastoma in Human Brain Organoids Cell Rep, 2020-06-09;31(10):107738. 2020-06-09 [PMID: 32521263] (Bioassay, Human)
• J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice J. Biol. Chem, 2017-03-10;0(0):. 2017-03-10 [PMID: 28283575] (Bioassay, Mouse)

Bioinformatics

• Gene Symbol: NLGN3
• Uniprot:
  • Q9NZ94()