Recombinant Human Netrin-4 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Netrin-4 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rrUNC5H2/Fc Chimera at 5 µg/mL (100 µL/well) can bind rhNetrin-4 with a linear range of 6-400 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Netrin-4 protein
  Val26-Lys628 , with a C-terminal 10-His tag
• Accession #: Q9HB63.2
• N-terminal Sequence: Val26
• Protein/Peptide Type: Recombinant Proteins
• Gene: NTN4
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 90 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 2 weeks, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Netrin-4 Protein, CF

• beta-Netrin
• hepar-derived netrin-like protein
• netrin 4
• Netrin4
• Netrin-4
• NTN4
• PRO3091

Background

Netrins/UNC-6 (netr: Sanskrit for “one who guides”) are a family of laminin-related small proteins that are involved in neurite outgrowth and axon guidance. Netrins bind to the DCC and UNC5 family of receptors to attract or repel axons. Human Netrin-4 is synthesized as a 628 amino acid (aa) precursor that contains a 19 aa signal sequence, a 443 aa laminin-related region containing an N-terminal laminin globular domain (domain VI) followed by 3 laminin EGF-like repeats, and a 166 aa C-terminal domain rich in basic aa residues that serves as a heparin binding site. Unlike human Netrin-1 which is reminiscent of the laminin gamma -chain, human Netrin-4 resembles the laminin beta -chain. Netrin-4 has been reported to exist as both a monomer and a dimer. The dimeric form was reported to be the less active of the two. Human Netrin-4 shares 31%, 29% and 25% aa sequence identity with human Netrin-1, 2 and G2, respectively; it shares 89% aa identity with mouse Netrin-4. Netrin-4 has widespread expression, occurring in nervous tissues such as embryonic floor plate and postnatal neurons such as cerebellar granule cells and hippocampal pyramidal cells. It also is found in non-neural tissues, such as adult Bowman’s capsule and medullary tubular epithelium in kidney, and embryonic pancreatic and intestinal epithelium, plus cells of the ureteric bud. Netrin-4 expression is often associated with basement membrane. Netrin-4 has been shown to initiate axon outgrowth from olfactory bulb explants (1 - 7).

1. McFarlane, S. (2000) Biochem. Cell. Biol. 78:563.
2. Kennedy, T.E. (2000) Biochem. Cell Biol. 78:569.
3. Yu, T.W. and C.I. Bargmann (2001) Nature Neurosci. 4(Suppl):1169.
4. Stein, E. et al. (2001) Science 291:1976.
5. Koch, M. et al. (2000) J. Cell Biol. 151:221.
6. Yin, Y. et al. (2000) Mech. Dev. 96:115.
7. Livesey, F.J. (1999) Cell. Mol. Life Sci. 56:62.

Publications for Netrin-4 (1254-N4/CF)(8)

Publications using 1254-N4/CF

• H Zhang, D Vreeken, DG Leuning, CS Bruikman, A Junaid, W Stam, RG de Bruin, WMPJ Sol, TJ Rabelink, BM van den Be, AJ van Zonnev, JM van Gils Netrin-4 expression by human endothelial cells inhibits endothelial inflammation and senescence The international journal of biochemistry & cell biology, 2021-02-23;134(0):105960. 2021-02-23 [PMID: 33636396] (Bioassay, Human)
• N Naldaiz-Ga, M Goicoechea, IM Aragón, V Pérez-Lópe, S Fuertes-Al, B Herrera-Im, A López de M, R de Luna-Di, PM Baptista, MA Fernández, MF Lara, A Izeta Isolation and characterization of myogenic precursor cells from human cremaster muscle Sci Rep, 2019-03-05;9(1):3454. 2019-03-05 [PMID: 30837559] (Bioassay, Human)
• L Li, Y Huang, Y Gao, T Shi, Y Xu, H Li, M Hyytiäinen, J Keski-Oja, Q Jiang, Y Hu, Z Du EGF/EGFR upregulates and cooperates with Netrin-4 to protect glioblastoma cells from DNA damage-induced senescence BMC Cancer, 2018-12-04;18(1):1215. 2018-12-04 [PMID: 30514230] (Bioassay, Human)
• AA Villanueva, P Falcón, N Espinoza, LS R, LA Milla, E Hernandez-, VA Torres, P Sanchez-Go, V Palma The Netrin-4/ Neogenin-1 axis promotes neuroblastoma cell survival and migration Oncotarget, 2017-02-07;8(6):9767-9782. 2017-02-07 [PMID: 28038459] (Bioassay, Human)
• Akino T, Han X, Nakayama H, McNeish B, Zurakowski D, Mammoto A, Klagsbrun M, Smith E Netrin-1 promotes medulloblastoma cell invasiveness and angiogenesis, and demonstrates elevated expression in tumor tissue and urine of patients with pediatric medulloblastoma. Cancer Res, 2014-05-08;74(14):3716-26. 2014-05-08 [PMID: 24812271] (Western Blot)
• Li L, Hu Y, Ylivinkka I, Li H, Chen P, Keski-Oja J, Hyytiainen M NETRIN-4 protects glioblastoma cells FROM temozolomide induced senescence. PLoS ONE, 2013-11-12;8(11):e80363. 2013-11-12 [PMID: 24265816] (Bioassay, Human)
• Sneddon , Julie B, Borowiak , Malgorza, Melton , Douglas Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme. Nature, 2012-10-07;491(7426):765-8. 2012-10-07 [PMID: 23041930] (Cell Culture, Human)
• Lejmi E, Leconte L, Pedron-Mazoyer S, Ropert S, Raoul W, Lavalette S, Bouras I, Feron JG, Maitre-Boube M, Assayag F, Feumi C, Alemany M, Jie TX, Merkulova T, Poupon MF, Ruchoux MM, Tobelem G, Sennlaub F, Plouet J Netrin-4 inhibits angiogenesis via binding to neogenin and recruitment of Unc5B. Proc. Natl. Acad. Sci. U.S.A., 2008-08-21;105(34):12491-6. 2008-08-21 [PMID: 18719102] (Bioassay, Human)

Bioinformatics

• Gene Symbol: NTN4
• Uniprot:
  • Q9HB63.2()