Recombinant Human MuSK Fc Chimera Protein, CF

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When Recombinant Human MuSK Fc Chimera (Catalog # 9810‑MK) iscoated at 2 μg/mL, Recombinant Human LRP-4 (Catalog # 5948-LR) binds withan ED50 of 0.075‑0.45 μg/mL in the presence of Recombinant Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human MuSK Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. In the presence of Recombinant Human Agrin (Catalog # 6624-AG), immobilized Recombinant Human MuSK Fc Chimera (2 µg/mL at 100 µL/well) binds Recombinant Human LRP-4 (Catalog # 5948-LR) with an ED50 of 0.075-0.45 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human MuSK protein
Human MuSK
(Leu24-Thr495)
Accession # O15146-1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Leu24
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
86-103 kDa, reducing conditions

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MuSK Fc Chimera Protein, CF

  • EC 2.7.10
  • EC 2.7.10.1
  • MGC126323
  • MGC126324
  • muscle, skeletal, receptor tyrosine kinase
  • MuSK
  • skeletal receptor tyrosine-protein kinase

Background

MuSK (muscle-specific kinase) is a 100 kDa type I transmembrane (TM) protein belonging to the receptor tyrosine kinase family (1). It contains a 472 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 353 cytoplasmic domain (1). Within the ECD, Human MuSK shares 91% and 92% aa sequence identity with mouse MuSK and rat MuSK, respectively. It is found in the postsynaptic membrane of skeletal muscle motor endplates (2). Human MuSK has multiple isoforms. One contains deletions at residues 307‑394 and 454‑461, while a second is a short soluble form that contains residues 120‑209 plus a unique 24 aa C‑terminal tail (3). MuSK binds the heparin sulfate proteoglycan agrin to promote acetylcholine receptor clustering. It has also been found to bind with low-density lipoprotein receptor-related protein 4 (LRP4) (4), Wnt ligands (5), biglycan (6), and ColQ (7). Recent studies have showed that MuSK does not bind agrin directly, but enhanced the MuSK-LRP4 interaction (7, 8). When Agrin binds to the N-Terminal region of LRP4, this promotes the association of LRP4 and MuSK, which then stimulates MuSK kinase activity (9, 10).
  1. Valenzuela, D.M. et al. (1995) Neuron. 15:573.
  2. Finn, A.J. et al. (2003) Nat. Neurosci. 6:717.
  3. Nasrin, F. et al. (2014) Sci. Rep. 4:6841.
  4. Kim, N. et al. (2008) Cell 135:334.
  5. Banerjee, S. et al. (2011) Development 138:3287.
  6. Amenta, A.R. et al. (2012) J Neurosci 32:2324.
  7. Otsuka, K. et al (2015) Scientific Reports. 5:13928.
  8. Zhang, B. et al. (2008) Neuron 60:285.
  9. Zhang, W. et al. (2011) J Biol Chem. 286(47):40624.
  10. Burden, S.J. et al. (2017) CSH Perspectives Biol. 5:a009167.

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