Recombinant Human Megalin/LRP-2 (C3) Protein, CF

• Reactivity: Hu
• Format: Carrier-Free

Recombinant Human Megalin/LRP-2 (C3) Protein, CF Summary

• Details of Functionality: Bioassay data are not available.
• Source: Mouse myeloma cell line, NS0-derived human Megalin/LRP2 protein Glu2698-Gly3110, with a C-terminal 6-His tag
• Accession #: P98164
• N-terminal Sequence: Glu2698
• Protein/Peptide Type: Innovator Recombinant Proteins
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity not tested
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 82-95 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 3 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Megalin/LRP-2 (C3) Protein, CF

• calcium sensor protein
• DBS
• DBSlow-density lipoprotein receptor-related protein 2
• EC 1.1.2.3
• EC 3.4.21.9
• Glycoprotein 330
• gp330
• Heymann nephritis antigen homolog
• low density lipoprotein receptor-related protein 2
• LRP2
• LRP-2
• Megalin

Background

Megalin, also known as the low-density lipoprotein receptor-related protein 2 (LRP2), is a large type I transmembrane cell surface protein. This glycoprotein is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney (1). The Megalin protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling. Mutations in this gene cause Donnai-Barrow Syndrome (DBS) and Facio-Oculoacoustico-Renal Syndrome (FOAR) (1). Megalin is consisting of a 25 amino acid (aa) probable N-terminal signal peptide sequence, a 4400 aa extracellular region, a 22 aa single transmembrane domain, and a 213 aa C-terminal cytoplasmic tail. The entire extracellular region is made up of 36 class A motifs of putative ligand-binding domains arranged in four distinct clusters, 16 growth factor repeats separated by 8 YWTD spacer regions, and 1 epidermal growth factor-like repeat (2). The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase (3). The amino acid 2698-3110 encodes the third class A motif cluster in human Megalin, termed Megalin C3. Human Megalin C3 shares 77% and 74% identity with mouse and rat Megalin C3.

1. Christensen, E. I. and Birn, H. (2002) Nat. Rev. Mol. Cell Biol 3:256.
2. Saito, A. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:9725
3. Kantarci, S. et al. (2007) Nat. Genet 39:957.

Bioinformatics

• Uniprot:
  • P98164()