Recombinant Human MARCO Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human MARCO Protein, CF Summary

• Details of Functionality: Measured by its ability to bind fluorescein-conjugated E. coli Bioparticles. Sankala, M. et al. (2002) J. Biol. Chem. 277:33378. The ED₅₀ for this effect is 0.3-1.5 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human MARCO protein
  Met79-Val520, with an N-terminal 6-His tag
• Accession #: Q9UEW3
• N-terminal Sequence: His
• Protein/Peptide Type: Recombinant Proteins
• Gene: MARCO
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 44.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 60-70 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 250 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MARCO Protein, CF

• macrophage receptor with collagenous structureSCARA2Scavenger receptor class A member 2
• MARCO
• member 2
• SCARA2

Background

MARCO (macrophage receptor with collagenous structure), also known as SCARA2, is an 80 kDa type II transmembrane glycoprotein in the class A scavenger receptor family (1). Human MARCO consists of a 43 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 456 aa extracellular domain (ECD) that includes a stalk region, a collagen‑like region, and one SRCR domain (2). Within the ECD, human MARCO shares 69% aa sequence identity with mouse and rat MARCO. MARCO is constitutively expressed on the surface of splenic and lymph node macrophages (3). Its expression can be induced on Kupffer cells, alveolar macrophages, and glial cells by microbial infection, chemical irritants, and Th1 polarizing factors (4‑6). The SRCR domain mediates binding of MARCO to its various ligands, while the collagen‑like region mediates assembly into a disulfide-linked trimer (2, 3, 7). MARCO binds bacterial LPS and lipoteichoic acid, modified LDL, CpG oligonucleotides, UGRP1, silica, and TiO₂ (3, 7‑10). It interacts in cis with the formyl peptide receptors FPR1 and FPRL1 on astrocytes and microglia (11). MARCO ligation promotes the production of inflammatory mediators by macrophages (8). MARCO mediated internalization of some ligands prevents their activation of cell surface TLR4 but enables their activation of intracellular TLR3 (12). MARCO contributes to the clearance of apoptotic cells and inhaled bacteria, mast cell mediated silicosis, and the amelioration of allergen or ozone induced lung inflammation (4, 13‑16). It is required for the organization of the splenic marginal zone and the interaction of splenic macrophages and B cells (17).

1. Sheetal, A. et al. (2008) J. Immunotoxicol. 5:151.
2. Elomaa, O. et al. (1998) J. Biol. Chem. 273:4530.
3. Elomaa, O. et al. (1995) Cell 80:603.
4. Dahl, M. et al. (2007) J. Clin. Invest. 117:757.
5. Jozefowski, S. et al. (2005) J. Immunol. 175:8032.
6. Braun, B.J. et al. (2011) J. Neuroinflamm. 8:11.
7. Chen, Y. et al. (2006) J. Biol. Chem. 281:12767.
8. Jozefowski, S. et al. (2006) J. Leukoc. Biol. 80:870.
9. Bin, L.-H. et al. (2003) J. Immunol. 171:924.
10. Hamilton, Jr., R.F. et al. (2006) J. Biol. Chem. 281:34218.
11. Brandenburg, L.-O. et al. (2010) J. Neurochem. 113:749.
12. Mukhopadhyay, S. et al. (2011) Blood 117:1319.
13. Arredouani, M. et al. (2004) J. Exp. Med. 200:267.
14. Rogers, N.J. et al. (2009) J. Immunol. 182:1982.
15. Brown, J.M. et al. (2007) Am. J. Respir. Cell Mol. Biol. 36:43.
16. Arredouani, M.S. et al. (2007) J. Immunol. 178:5912.
17. Karlsson, M.C.I. et al. (2003) J. Exp. Med. 198:333.

Bioinformatics

• Gene Symbol: MARCO
• Uniprot:
  • Q9UEW3()