Recombinant Human MAGP-2/MFAP5 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human MAGP-2/MFAP5 Protein, CF Summary

• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of BUD‑8 human fibroblast cells at 10 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
• Source: Mouse myeloma cell line, NS0-derived human MAGP-2/MFAP5 protein
  Ile22-Leu173 with a C-terminal 6-His tag
• Accession #: Q13361
• N-terminal Sequence: Ile22 & Ser154
• Structure / Form: Monomer
• Protein/Peptide Type: Recombinant Proteins
• Gene: MFAP5
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 25-35 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 400 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human MAGP-2/MFAP5 Protein, CF

• MAGP2
• MAGP-2
• MAGP2MFAP-5
• MFAP5
• Microfibril-associated glycoprotein 2
• microfibrillar associated protein 5
• microfibrillar-associated protein 5
• MP25
• MP25microfibril-associated glycoprotein-2

Background

MAGP-2 (Microfibril-associated glycoprotein 2), also called MFAP5 (microfibril associated protein 5) is a secreted, cell-associated 25 kDa mammalian member of the MFAP family of proteins (1). Human MAGP-2 cDNA encodes 173 amino acids (aa) including a 21 aa signal sequence, an RDG motif (aa 30‑32; absent in MAGP-1) and a central cysteine‑rich binding domain (aa 84‑140) (1‑3). One potential splice variant shows a 10 aa deletion between aa 73‑82 that includes the only potential glycosylation site (4). Mature human MAGP-2 shares 80%, 82%, 82%, 84% and 85% aa identity with mouse, rat, bovine, porcine and canine MAGP-2, respectively. Both MAGP-1 and MAGP-2 bind and covalently crosslink fibrillins 1 and 2  through the binding domain, but MAGP-2 has more limited distribution and binds a more limited set of extracellular matrix proteins (1‑3). However, the MAGP-2 RGD motif binds to the integrin alpha v beta 3, allowing cell attachment to microfibrils (5). MAGP‑2 is thought to facilitate microfibril assembly and induce elastin fiber formation (1‑3, 6). Through its binding to EGF repeats, MAGP-2 mediates the metalloproteinase-dependent release of Jagged1 and the metalloproteinase-independent release of Notch extracellular domains (7, 8). In endothelial cells, MAGP-2 promotes angiogenic sprouting through inhibiting Notch signaling, binding integrin  alpha v beta 3 (which then enhances VEGF signaling), and/or enhancing endothelial cell response to FGF basic and EGF (9, 10). In ovarian cancer, MAGP-2 expression is associated with cancer cell survival, increased microvessel density, and poor prognosis (11). Skin MAGP-2 expression is increased in human scleroderma and the tight skin (TSK) mouse; MAGP‑2 may contribute to abnormal collagen accumulation in these conditions by increasing the half-life of type I collagen (12, 13). Fibrillin-1 mutations within the region that binds MAGP-2 can be found in Marfan syndrome (6).

1. Gibson, M.A. et al. (1996) J. Biol. Chem. 271:1096.
2. Hanssen, E. et al. (2004) J. Biol. Chem. 279:29185.
3. Segade, F. et al. (2002) J. Biol. Chem. 277:11050.
4. Genbank protein Accession # AAQ18021.
5. Gibson, M.A. et al. (1999) J. Biol. Chem. 274:13060.
6. Lemaire, R. et al. (2007) J. Biol. Chem. 282:800.
7. Nehring, L.C. et al. (2005) J. Biol. Chem. 280:20349.
8. Miyamoto, A. et al. (2006) J. Biol. Chem. 281:10089.
9. Albig, A.R. et al. (2008) Microvasc. Res. 76:7.
10. Albig, A.R. et al. (2007) Angiogenesis 10:197.
11. Mok, S.C. et al. (2009) Cancer Cell 16:521.
12. Lemaire, R. et al. (2005) Arthritis Rheumatol. 52:1812.
13. Ito, S. et al. (2006) Am.J. Respir. Cell Mol. Biol. 34:688.

Publications for MAGP-2/MFAP5 (4914-MG)(1)

Publication using 4914-MG

• Han, C;Leonardo, TR;Romana-Souza, B;Shi, J;Keiser, S;Yuan, H;Altakriti, M;Ranzer, MJ;Ferri-Borgogno, S;Mok, SC;Koh, TJ;Hong, SJ;Chen, L;DiPietro, LA; Microfibril-associated protein 5 and the regulation of skin scar formation Scientific reports 2023-05-30 [PMID: 37253753] (Bioassay, Human)

Bioinformatics

• Gene Symbol: MFAP5
• Uniprot:
  • Q13361(Human)