Recombinant Human LYVE-1 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human LYVE-1 Protein, CF Summary

• Details of Functionality: Measured by its ability to bind hyaluronan in a functional ELISA.
• Source: Mouse myeloma cell line, NS0-derived human LYVE-1 protein
  Ser24-Thr238, with a C-terminal 6-His tag
• Accession #: Q9Y5Y7
• N-terminal Sequence: Ser24
• Protein/Peptide Type: Recombinant Proteins
• Gene: LYVE1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-60 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LYVE-1 Protein, CF

• cell surface retention sequence binding protein-1
• Cell surface retention sequence-binding protein 1
• CRSBP1
• CRSBP-1
• extracellular link domain containing 1
• extracellular link domain-containing 1
• Extracellular link domain-containing protein 1
• HAR
• Hyaluronic acid receptor
• lymphatic vessel endothelial hyaluronan receptor 1
• lymphatic vessel endothelial hyaluronic acid receptor 1
• LYVE1
• LYVE-1
• UNQ230/PRO263
• XLKD1

Background

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D-glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 - 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity.

LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 - 11).

1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Publications for LYVE-1 (2089-LY)(1)

Publication using 2089-LY

• EJ Lim, Y Suh, KC Yoo, JH Lee, IG Kim, MJ Kim, JH Chang, SG Kang, SJ Lee Tumor-associated mesenchymal stem-like cells provide extracellular signaling cue for invasiveness of glioblastoma cells Oncotarget, 2017-01-03;8(1):1438-1448. 2017-01-03 [PMID: 27903965] (Bioassay, Human)

Bioinformatics

• Gene Symbol: LYVE1
• Uniprot:
  • Q9Y5Y7()