Recombinant Human Lymphotoxin alpha2/beta1 Protein

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Lymphotoxin alpha2/beta1 Protein Summary

Details of Functionality
Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells. The ED50 for this effect is 0.5‑3 ng/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Lymphotoxin protein
Leu35-Leu205 (LT alpha ) & Leu54-Gly244 (LT beta ), both with an N-terminal Met & Thr41 ‑ Leu205 (LT alpha )
Accession #
N-terminal Sequence
Met & Thr41 (LT alpha ) & Met (LT beta )
Structure / Form
Noncovalently-linked heterotrimer
Protein/Peptide Type
Recombinant Proteins
Gene
LTA
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa (LT alpha ), 18.5 kDa ( LT beta ).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
18-26 kDa, reducing conditions
Publications
Read Publications using
679-TX in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Lymphotoxin alpha2/beta1 Protein

  • LT
  • Lymphotoxin alpha
  • Lymphotoxin
  • tnfb
  • TNFSF1
  • TNLG1E

Background

Lymphotoxin-alpha (LT-alpha ), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT-beta ) are members of the TNF family. LT-alpha cDNA encodes a 205 amino acid secreted soluble protein with a 34 amino acid signal sequence. LT-beta cDNA encodes a 244 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 19 - 48), and an extracellular domain (residues 49 - 244). Secreted LT-alpha assembles as a soluble homotrimer, LT-alpha 3. In addition, secreted LT-alpha also complexes with the membrane associated LT-beta to generate two types of heterotrimers, LT-alpha 1/ beta 2 and LT-alpha 2/ beta 1 (1). The soluble LT-alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT-alpha 1/ beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT-alpha 2/ beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT-alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naive CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).

  1. Browning et al. (1993) Cell 72:847.
  2. Ettinger et al. (1996) PNAS 93:13102.
  3. Cuff et al. (1998) J. Immunol. 161:6853.
  4. Browning et al. (1997) J. Immunol. 159:3288.
  5. Gramaglia et al. (1999) J. Immunol. 162:1333.

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Publications for Lymphotoxin (679-TX)(3)

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