Recombinant Human LRP-6 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Human LRP-6 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. rhLRP-6/Fc Chimera binds rhDkk-1, biotin with an apparent KD <25 nM. Biotinylated rmWnt-3a immobilized on a streptavidin-coated plate at 1 µg/mL can bind rhLRP-6/Fc Chimera with a linear range of 0.2-10 µg/mL.
Optimal concentrations should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived human LRP-6 protein
Human LRP-6
(Ala20 - Pro1368)
Accession # O75581
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Ala20
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
LRP6
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.1 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
180 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
200 kDa, reducing conditions
Publications
Read Publications using
1505-LR in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LRP-6 Fc Chimera Protein, CF

  • ADCAD2
  • FLJ90062
  • FLJ90421
  • low density lipoprotein receptor-related protein 6
  • low-density lipoprotein receptor-related protein 6
  • LRP6
  • LRP-6

Background

The low-density lipoprotein (LDL) receptor-related protein 5 (LRP5) and LRP6 constitute a distinct subgroup of the LDL receptor family. Both LRP5 and LRP6 are type I transmembrane proteins that function as co-receptors with Frizzled (FZD) in the canonical Wnt signaling pathway (1, 2). LRP6 cDNA encodes a 1613 amino acid residue (aa) precursor with a 19 aa signal sequence, a 1353 aa extracellular region, a 23 aa transmembrane (TM) segment and a 218 aa cytoplasmic domain (3). The extracellular region contains four N-terminal cysteine-rich EGF-like repeats, followed by three cysteine-rich LDLR repeats. This pattern of the EGF and LDLR repeat arrangement is different than that typically found in other LDL receptor family proteins. The intracellular region of LRP6 contains protein-protein interaction motifs and is required for canonical Wnt signal transduction (4). Human LRP-6 shares 98% and 74% aa sequence identity with mouse LRP-6 and human LRP-5, respectively. Based on the current model, canonical Wnt signaling requires the interaction of Wnt with FZD and LRP to form a trimeric complex which signals downstream to stabilize cytoplasmic  beta -catenin. The stabilized beta -catenin is then translocated to the nucleus where it complexes with the transcription factor LEF/TCF to regulate the transcription of target genes (5). LRP6 has also been shown to interact with the Dickkopf proteins (DKKs), which are modulators of Wnt signaling (6 - 8). Binding of DKK-1 to LRP6 dissociates LRP6 from FZD, and antagonizes the formation of the functional receptor complex. On cells where the transmembrane proteins Kremens are also present, a ternary complex of LRP6, DKK-1 and Kremen is formed to trigger the internalization of the complex and removal LRP6 from the cell surface. Thus, DKK-1 and Kremen function synergistically to antagonize LRP6-mediated Wnt activity. Although DKK-2 also functions as a Wnt antagonist on cells that express Kremen, DKK-2 binding to LRP-6 in the absence of Kremen activates rather than inhibits LRP mediated beta -catenin signaling (9, 10).

  1. Howell, B.W. and J. Herz (2001) Curr. Op. Neurobiology 11:74.
  2. Pinson, K.I. et al. (2000) Nature 407:535.
  3. Brown, S.D. et al. (1998) Biochem. Biophys. Res. Commun. 248:879.
  4. Tamai, K. et al. (2000) Nature 407:530.
  5. Schweizer, L. and H. Varmus (2003) BMC Cell Biology 4:4.
  6. Mao, B. et al. (2001) Nature 411:321.
  7. Semenov, M.V. et al. (2001) Curr. Biol. 11:951.
  8. Bafico, A. et al. (2001) Nature Cell Biol. 3:683.
  9. Zorn, A.M. (2001) Curr. Biol. 11:R592.
  10. Mao, B. et al. (2002) Nature 417:664.

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Publications for LRP-6 (1505-LR)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: Antibody Selection, Binding Assay, Bioassay, ELISA capture.


Filter By Application
Antibody Selection
(1)
Binding Assay
(1)
Bioassay
(1)
ELISA capture
(2)
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Human
(4)
Mouse
(1)
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Bioinformatics

Gene Symbol LRP6
Uniprot