Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. When Recombinant Human Glucosylceramidase/GBA (Catalog # 7410-GHB) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human LIMPII/SR-B2 Fc Chimera (Catalog # 1966-LM) binds with an ED50 of 100-800 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human LIMPII/SR-B2 protein
  

  Human LIMPII (Arg27-Thr432) Accession # Q14108	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #: Q14108
• N-terminal Sequence: Arg27
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: SCARB2
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 73 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 105-115 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human LIMPII/SR-B2 Fc Chimera Protein, CF

• AMRF
• CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2(lysosomal integral membrane protein II)
• CD36 antigen
• CD36L2
• CD36L2LIMP II
• HLGP85
• LGP85
• LIMP-2,85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2
• LIMPII
• LIMPIICD36 antigen-like 2
• LPG85
• lysosome membrane protein 2
• Lysosome membrane protein II
• SCARB2
• Scavenger receptor class B member 2,85 kDa lysosomal membrane sialoglycoprotein
• scavenger receptor class B, member 2
• SRB2
• SR-B2
• SR-BII

Background

LIMPII (Lysosomal Integral Membrane Protein II), also known as LPG85 (85 kDa lysosomal membrane sialoglycoprotein) and as CD36 antigen-like 2 (CD36L2), is a major lysosomal membrane protein. It belongs to the scavenger receptor class B subfamily and is designated member 2 (SR-B2). Other mammalian members of this family include SR-B1 (alternatively known as Cla-1 and CD36L1), and SR-B3 (CD36) (1-3). SR-B/CD36 family members are type III integral membrane proteins with an N- as well as a C-terminal cytoplasmic tail, and a large extracellular (or lumenal in the case of LIMPII) loop containing similarly spaced cysteine residues and multiple glycosylation sites. The C-terminal cytoplasmic tail has a di-leucine-based motif that mediates effective lysosomal targeting. LIMPII is widely expressed on all tissues and cell types so far examined. It is also expressed on the surface of activated platelets. LIMPII binds thrombospondin-1, but the biological significance of this interaction is not known. LIMPII-thrombospondin interaction may contribute to the pro-adhesive changes of activated platelets during coagulation, and inflammation (1). Overexpression of LIMPII causes an enlargement of early endosomes and late endosomes, suggesting that LIMPII may play a role in lysosome/endosome biogenesis (4). Mice deficient in LIMPII are impaired in membrane transport processes, resulting in ureteric pelvic junction obstruction, deafness and peripheral neuropathy (5).

1. Crombie, R. and R. Silverstein (1998) J. Biol. Chem. 273:4855. 
2. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785.
3. Eskelinen, E-L. et al. (2003) Trends in Cell Biology 13:137.
4. Kuronita, T. et al. (2002) J. Cell Sci. 115:4117.
5. Gamp, A-C. et al. (2003) Human Molecular Genetics 12:631.

Publications for LIMPII/SR-B2 (1966-LM)(4)

Publications using 1966-LM

• Ku Z, Ye X, Shi J, Wang X, Liu Q, Huang Z Single Neutralizing Monoclonal Antibodies Targeting the VP1 GH Loop of Enterovirus 71 Inhibit both Virus Attachment and Internalization during Viral Entry. J Virol, 2015-09-23;89(23):12084-95. 2015-09-23 [PMID: 26401034] (Bioassay, Human)
• Yamayoshi S, Iizuka S, Yamashita T Human SCARB2-dependent infection by coxsackievirus A7, A14, and A16 and enterovirus 71. J. Virol., 2012-03-21;86(10):5686-96. 2012-03-21 [PMID: 22438546] (Immunoprecipitation, Virus)
• Yamayoshi S, Koike S Identification of a Human SCARB2 Region That Is Important for Enterovirus 71 Binding and Infection. J. Virol., 2011-03-09;85(10):4937-46. 2011-03-09 [PMID: 21389126] (Immunoprecipitation, Human)
• Yamayoshi S, Yamashita Y, Li J, Hanagata N, Minowa T, Takemura T, Koike S Scavenger receptor B2 is a cellular receptor for enterovirus 71. Nat. Med., 2009-06-21;15(7):798-801. 2009-06-21 [PMID: 19543282] (Bioassay, Human)

Bioinformatics

• Gene Symbol: SCARB2
• Uniprot:
  • Q14108()