Recombinant Human Klotho (aa 34-981) Protein, CF


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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human Klotho (aa 34-981) Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using BaF3 mouse pro‑B cells transfected with human FGF RIIIc. The ED50 for this effect is 0.025-0.1 μg/mL in the presence of Recombinant Human FGF‑23 (Catalog # 2604-FG) and Heparin.
Mouse myeloma cell line, NS0-derived human Klotho protein
Glu34-Ser981, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
109.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
120-130 kDa, reducing conditions
Read Publications using
5334-KL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
Supplied as a 0.2 μm filtered solution in MES, Glycerol and EDTA.
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Klotho (aa 34-981) Protein, CF

  • EC 3.2.1
  • EC
  • KL
  • Klotho


Klotho, also called Klotho-alpha , is the founding member of the Klotho family within the glycosidase-1 superfamily (1, 2). Klotho is expressed in areas concerned with calcium regulation, predominantly in the kidney distal convoluted tubules, but also in the brain choroid plexus (which produces cerebrospinal fluid) and the parathyroid (1). The 1012 amino acid (aa) type I transmembrane protein contains a 32 aa signal sequence, a 948 aa extracellular domain (ECD) containing two extracellular glycosidase-like domains, a 21 aa transmembrane domain and a 10 aa intracellular domain. Within the ECD, human Klotho shares 87%, 90%, 90% and 86% aa identity with mouse, rat, bovine and equine Klotho, respectively. A 130 kDa form found in plasma and cerebrospinal fluid is produced by proteolytic cleavage of the glycosylated 135 kDa full-length Klotho (3, 4). A truncated 549 aa isoform predicted by alternative mRNA splicing has been detected in human and mice (3, 4). A prominent intracellular 120 kDa form of Klotho is localized to endoplasmic reticulum and Golgi membranes (4). The phenotype of Klotho-deficient mice resembles premature aging, including arteriosclerosis, osteoporosis, skin atrophy, infertility, emphysema and premature death (2). Conversely, excess Klotho extends lifespan (5). Klotho acts as a cofactor for interaction of FGF-23 with FGF R1 (6). This interaction negatively regulates 1 alpha -hydroxylase, the rate-limiting enzyme in the synthesis of 1,25(OH)2D3 (vitamin D) (7). Klotho deficient mice show severe hyperphosphatemia and ectopic calcification of soft tissues due to excess vitamin D
(2-7). Both Klotho and Klotho beta are cofactors for FGF19 binding (8). Klotho also shows glucuronidase activity which activates the renal ion channel TRPV5 to reabsorb urinary calcium (9). Klotho has been reported to downregulate insulin or IGF-1 signaling in adipocytes, to bind and antagonize Wnt molecules, and to facilitate release of parathyroid hormone (10-12).

  1. Nabeshima, Y. (2006) Sci. Aging Knowl. Environ. 8:pe11.
  2. Kuro-o, M. et al. (1997) Nature 390:45.
  3. Shiraki-Iida, T. et al. (1998) FEBS Lett. 424:6.
  4. Imura, A. et al. (2004) FEBS Lett. 565:143.
  5. Kurosu, H. et al. (2005) Science 309:1829.
  6. Kurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
  7. Tsujikawa, H. et al. (2003) Mol. Endocrinol. 17:2393.
  8. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
  9. Chang, Q. et al. (2005) Science 310:490.
  10. Yamamoto, M. et al. (2005) J. Biol. Chem. 280:38029.
  11. Liu, H. et al. (2007) Science 317:803.
  12. Imura, A. et al. (2007) Science 316:1615.

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Publications for Klotho (5334-KL)(19)

We have publications tested in 3 confirmed species: Human, Mouse, N/A.

We have publications tested in 4 applications: Bioassay, Cell Culture, ELISA (Control), ELISA Capture.

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Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using 5334-KL Applications Species
J Suk Kang, SS Son, JH Lee, SW Lee, AR Jeong, ES Lee, SK Cha, CH Chung, EY Lee Protective effects of klotho on palmitate-induced podocyte injury in diabetic nephropathy PLoS ONE, 2021;16(4):e0250666. 2021 [PMID: 33891667] (Bioassay, Mouse) Bioassay Mouse
S Jadhav, S Tripathi, A Chandrekar, SS Waikar, LL Hsiao A novel antibody for the detection of alternatively spliced secreted KLOTHO isoform in human plasma PLoS ONE, 2021;16(1):e0245614. 2021 [PMID: 33481901] (ELISA Capture, Human) ELISA Capture Human
S Batlahally, A Franklin, A Damianos, J Huang, P Chen, M Sharma, J Duara, D Keerthy, R Zambrano, LA Shehadeh, EC Martinez, MJ DeFreitas, S Kulandavel, CL Abitbol, M Freundlich, RM Kanashiro-, A Schmidt, M Benny, S Wu, KK Mestan, KC Young Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants Sci Rep, 2020;10(1):12368. 2020 [PMID: 32704023] (Bioassay, Human) Bioassay Human
W Kawarazaki, R Mizuno, M Nishimoto, N Ayuzawa, D Hirohama, K Ueda, F Kawakami-M, S Oba, T Marumo, T Fujita Salt causes aging-associated hypertension via vascular Wnt5a under Klotho deficiency J. Clin. Invest., 2020;0(0):. 2020 [PMID: 32597829] (Cell Culture, Human) Cell Culture Human
A Olejnik, A Krzywonos-, M Banaszkiew, I Bil-Lula Klotho protein contributes to cardioprotection during ischaemia/reperfusion injury J. Cell. Mol. Med., 2020;0(0):. 2020 [PMID: 32319182] (Bioassay, Human) Bioassay Human
E The, Q Yao, P Zhang, Y Zhai, L Ao, DA Fullerton, X Meng Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells Cells, 2020;9(2):. 2020 [PMID: 32046115] (Cell Culture, Human) Cell Culture Human
C Grange, E Papadimitr, V Dimuccio, C Pastorino, J Molina, R O'Kelly, LJ Niedernhof, PD Robbins, G Camussi, B Bussolati Urinary Extracellular Vesicles Carrying Klotho Improve the Recovery of Renal Function in an Acute Tubular Injury Model Mol. Ther., 2019;0(0):. 2019 [PMID: 31818691] (Cell Culture, Human) Cell Culture Human
J Miao, J Liu, J Niu, Y Zhang, W Shen, C Luo, Y Liu, C Li, H Li, P Yang, Y Liu, FF Hou, L Zhou Wnt/?-catenin/RAS signaling mediates age-related renal fibrosis and is associated with mitochondrial dysfunction Aging Cell, 2019;0(0):e13004. 2019 [PMID: 31318148] (Bioassay, Human) Bioassay Human
A Sahu, H Mamiya, SN Shinde, A Cheikhi, LL Winter, NV Vo, D Stolz, V Roginskaya, WY Tang, C St Croix, LH Sanders, M Franti, B Van Houten, TA Rando, A Barchowsky, F Ambrosio Age-related declines in ?-Klotho drive progenitor cell mitochondrial dysfunction and impaired muscle regeneration Nat Commun, 2018;9(1):4859. 2018 [PMID: 30451844] (Bioassay, Mouse) Bioassay Mouse
T Arbel Rubi, S Shahmoon, E Zigmond, T Etan, K Merenbakh-, M Pasmanik-C, G Har-Zahav, I Barshack, GW Vainer, N Skalka, R Rosin-Arbe, C Varol, T Rubinek, I Wolf Klotho suppresses colorectal cancer through modulation of the unfolded protein response Oncogene, 2018;0(0):. 2018 [PMID: 30232408] (Bioassay, Human) Bioassay Human
Show All 19 Publications.

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Gene Symbol KL