Recombinant Human ISG15 E1/UBE1L Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human ISG15 E1/UBE1L Protein, CF Summary

• Details of Functionality: Recombinant Human ISG15 Activating Enzyme is a member of the ISG15-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a ISG15-conjugating (E2) enzyme and a ISG15 ligase (E3) to conjugate ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 Activating Enzyme concentration of 50-200 nM.
• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ISG15 Activating Enzyme/UBE1L protein
• Accession #: P41226
• Protein/Peptide Type: Recombinant Enzymes
• Gene: UBA7
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Theoretical MW: 112 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer:

  X mg/ml (X µM) in 50 mM Hepes, pH 8.0, 100 mM NaCl, 1 mM DTT

• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ISG15 E1/UBE1L Protein, CF

• D8
• D8UBA1, ubiquitin-activating enzyme E1 homolog B
• ISG15 Activating Enzyme/UBE1L
• UBA1B
• UBA7
• UBA7, ubiquitin-activating enzyme E1
• UBE1L
• UBE2
• UBE2MGC12713
• Ubiquitin-activating enzyme 7
• Ubiquitin-activating enzyme E1 homolog
• ubiquitin-activating enzyme E1-like
• ubiquitin-activating enzyme E1-related protein
• ubiquitin-activating enzyme-2
• ubiquitin-like modifier activating enzyme 7
• ubiquitin-like modifier-activating enzyme 7

Background

Interferon-stimulated Gene 15 (ISG15) Activating Enzyme (E1), also known as Ubiquitin-activating Enzyme 1-like (UBE1L), UBA7, and UBE2, is a 1012 amino acid (aa) member of the Ubiquitin-activating enzyme family with a predicted molecular weight of 111 kDa. The mouse and rat ISG15 E1/UBE1L orthologs share 80% aa sequence identity with the human protein. ISG15 E1/UBE1L catalyzes the activation of the C-terminal carboxyl group of the small Ubiquitin-like modifier ISG15. Like other E1 enzymes, ISG15 E1/UBE1L contains a conserved ATP-binding domain and an active site cysteine residue, Cys599 in humans. It is expressed in multiple tissues in response to IFN-alpha or -beta, but the covalent attachment of ISG15 to protein substrates (ISGylation) is not required for IFN-alpha or -beta signaling (1,2). In addition, ISG15 E1/UBE1L knockout mice appear to have normal embryonic development and fertility (2). However, it has been suggested that ISG15 E1/UBE1L may play a role in viral immunity as these knockout mice demonstrate increased susceptibility to influenza infection (3). ISG15 E1/UBE1L has also been shown to modulate erythroid differentiation and mediate retinoid-induced differentiation of acute promyelocytic leukemia cells (4,5). ISG15 E1/UBEL1 is downregulated in lung cancer and has been shown to downregulate Cyclin D in lung cancer cells (6,7).

1. Yuan, W. & R.M. Krug (2001) EMBO J. 20:362.
2. Kim, K.I. et al. (2006) Mol. Cell. Biol. 26:472.
3. Lai, C. et al. (2009) J. Virol. 83:1147.
4. Pitha-Rowe, I. et al. (2004) J. Biol. Chem. 279:18178.
5. Maragno, A.L. et al. (2011) PLoS One 6:e26068.
6. Pitha-Rowe, I. et al. (2004) Cancer Res. 64:8109.
7. Kok, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90:6071.

Publications for ISG15 Activating Enzyme/UBE1L (E-309)(1)

Publication using E-309

• Sun J, Zhou W, Kaliappan K, Nawaz Z, Slingerland J ERalpha phosphorylation at Y537 by Src triggers E6-AP-ERalpha binding, ERalpha ubiquitylation, promoter occupancy, and target gene expression. Mol Endocrinol, 2012;26(9):1567-77. 2012 [PMID: 22865929] (Bioassay, Human)

Bioinformatics

• Gene Symbol: UBA7
• Uniprot:
  • P41226(Human)