Recombinant Human ISG15 E1/UBE1L Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Enzyme Activity
Format
Carrier-Free

Order Details

Recombinant Human ISG15 E1/UBE1L Protein, CF Summary

Details of Functionality
Recombinant Human ISG15 Activating Enzyme is a member of the ISG15-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a ISG15-conjugating (E2) enzyme and a ISG15 ligase (E3) to conjugate ISG15 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human ISG15 Activating Enzyme concentration of 50-200 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ISG15 Activating Enzyme/UBE1L protein
Accession #
Protein/Peptide Type
Recombinant Enzymes
Gene
UBA7
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

Theoretical MW
112 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
E-309 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Buffer

X mg/ml (X µM) in 50 mM Hepes, pH 8.0, 100 mM NaCl, 1 mM DTT

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human ISG15 E1/UBE1L Protein, CF

  • D8
  • D8UBA1, ubiquitin-activating enzyme E1 homolog B
  • ISG15 Activating Enzyme/UBE1L
  • UBA1B
  • UBA7
  • UBA7, ubiquitin-activating enzyme E1
  • UBE1L
  • UBE2
  • UBE2MGC12713
  • Ubiquitin-activating enzyme 7
  • Ubiquitin-activating enzyme E1 homolog
  • ubiquitin-activating enzyme E1-like
  • ubiquitin-activating enzyme E1-related protein
  • ubiquitin-activating enzyme-2
  • ubiquitin-like modifier activating enzyme 7
  • ubiquitin-like modifier-activating enzyme 7

Background

Interferon-stimulated Gene 15 (ISG15) Activating Enzyme (E1), also known as Ubiquitin-activating Enzyme 1-like (UBE1L), UBA7, and UBE2, is a 1012 amino acid (aa) member of the Ubiquitin-activating enzyme family with a predicted molecular weight of 111 kDa. The mouse and rat ISG15 E1/UBE1L orthologs share 80% aa sequence identity with the human protein. ISG15 E1/UBE1L catalyzes the activation of the C-terminal carboxyl group of the small Ubiquitin-like modifier ISG15. Like other E1 enzymes, ISG15 E1/UBE1L contains a conserved ATP-binding domain and an active site cysteine residue, Cys599 in humans. It is expressed in multiple tissues in response to IFN-alpha or -beta, but the covalent attachment of ISG15 to protein substrates (ISGylation) is not required for IFN-alpha or -beta signaling (1,2). In addition, ISG15 E1/UBE1L knockout mice appear to have normal embryonic development and fertility (2). However, it has been suggested that ISG15 E1/UBE1L may play a role in viral immunity as these knockout mice demonstrate increased susceptibility to influenza infection (3). ISG15 E1/UBE1L has also been shown to modulate erythroid differentiation and mediate retinoid-induced differentiation of acute promyelocytic leukemia cells (4,5). ISG15 E1/UBEL1 is downregulated in lung cancer and has been shown to downregulate Cyclin D in lung cancer cells (6,7).

  1. Yuan, W. & R.M. Krug (2001) EMBO J. 20:362.
  2. Kim, K.I. et al. (2006) Mol. Cell. Biol. 26:472.
  3. Lai, C. et al. (2009) J. Virol. 83:1147.
  4. Pitha-Rowe, I. et al. (2004) J. Biol. Chem. 279:18178.
  5. Maragno, A.L. et al. (2011) PLoS One 6:e26068.
  6. Pitha-Rowe, I. et al. (2004) Cancer Res. 64:8109.
  7. Kok, K. et al. (1993) Proc. Natl. Acad. Sci. USA 90:6071.

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E-309
Species: Hu
Applications: Enzyme Activity

Publications for ISG15 Activating Enzyme/UBE1L (E-309)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol UBA7
Uniprot