Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Collagen IV at 10 µg/mL can bind rhIntegrin a3/ beta 1 with an apparent K_d <2 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human Integrin alpha 3 beta 1/VLA-3 protein
  

  Human Integrin alpha 3 (Phe33-Glu991) Accession # P26006	acidic tail
  Human Integrin beta 1 (Glu21-Asp728) Accession # P05556	basic tail
  N-terminus	C-terminus

• Accession #: P26006 (Integrin alpha 3) & P05556 (Integrin beta 1)
• N-terminal Sequence: Phe33 (Integrin alpha 3) & No results obtained: Gln21 predicted (Integrin beta 1)
• Protein/Peptide Type: Recombinant Proteins
• Gene: ITGA3
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 110.5 kDa (Integrin alpha 3), 82.3 kDa (Integrin beta 1).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 150 kDa and 125 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Integrin alpha 3 beta 1/VLA-3 Protein, CF

• Integrin alpha 3 beta 1
• VLA3
• VLA-3

Background

VLA-3 (Very Late Antigen 3) is a member of the integrin family, beta 1 subfamily, of cell membrane adhesion molecules (1-3). Integrins are nondisulfide-linked transmembrane (TM) heterodimers that contain an alpha - and beta -subunit (1). VLA-3 is composed of an alpha 3 and beta 1 subunit. The alpha 3/CD49c subunit is a 130-150 kDa type I TM glycoprotein. It only associates with the beta 1 integrin subunit. It is synthesized as a 1051 amino acid (aa) precursor that undergoes proteolytic cleavage to generate a disulfide-linked 110 kDa, 843 aa extracellular heavy chain and a 30 kDa, 176 aa TM/cytoplasmic light chain (1, 4, 5, 6). The heavy chain contains seven 60 aa repeats that fold into a propeller-like structure (7). Sequences involving the first three repeats are associated with ligand binding (1). The light chain has two cytoplasmic alternate splice forms. The A form cytoplasmic domain is 52 aa, while the B form cytoplasmic domain is 37 aa (5). Human alpha 3 heavy chain is 88% aa identical to mouse heavy chain. The human beta 1/CD29 subunit is a 130-140 kDa type I transmembrane glycoprotein (8). Unlike the alpha -subunit, it does not undergo proteolytic cleavage. The molecule contains a 708 aa extracellular region, a 23 aa TM segment, and a 47 aa cytoplasmic domain. The extracellular region contains one von Willebrand factor-like A domain and four cysteine-rich repeats. The cytoplasimc domain shows five alternate splice forms (A, B, C1, C2 and D), all which vary by 12 to 48 amino acids (9, 10). Although the functions of all are unclear, the B isoform appears to be dominant negative to the A isoform (11). Human beta 1 extracellular domain is 92%, 94%, 93% and 96% aa identical to the extracellular regions of beta 1 in rat, bovine, mouse and feline, respectively. VLA-3 is known to bind fibronectin, collagen, and laminin-1, 5, 8, 10 and 11 (1). It also binds tetraspanins such as CD9, CD63 and CD151. CD151 binding may actually stabilize VLA-3, enabling it to bind to additional factors (12).

1. Tsuji, T. et al. (2004) J. Membr. Biol. 200:115.
2. Gu, J. and N. Taniguchi (2004) Glycoconj. J. 21:9.
3. Kreidberg, J.A. (2000) Curr. Opin. Cell Biol. 12:548.
4. Takada, Y. et al. (1991) J. Cell. Biol. 115:257.
5. de Melker, A.A. et al. (1997) Lab. Invest. 76:547.
6. Krokhin, O.V. et al. (2003) Biochemistry 42:12950.
7. Springer, T.A. (2002) Curr. Opin. Struct. Biol. 12:802.
8. Argraves, W.S. et al. (1987) J. Cell Biol. 105:1183.
9. Svineng, G. et al. (1998) Biochem. J. 330:1255.
10. van der Flier, A. et al. (1995) FEBS Lett. 369:340.
11. Balzac, F. et al. (1994) J. Cell Biol. 127:557.
12. Nishiuchi, R. et al. (2005) Proc. Natl. Acad. Sci. USA 102:1939.

Publications for Integrin alpha 3 beta 1/VLA-3 (2840-A3)(5)

Publications using 2840-A3

• Boudko, SP;Konopka, EH;Kim, W;Taga, Y;Mizuno, K;Springer, TA;Hudson, BG;Moy, TI;Lin, FY; A recombinant technique for mapping functional sites of hetero-trimeric collagen helices: collagen IV CB3 fragment as a prototype for integrin binding The Journal of biological chemistry 2023-06-09 [PMID: 37302550] (Bioassay, N/A)
• EJ Hunter, SW Hamaia, PS Kim, JM Malcor, RW Farndale The effects of inhibition and siRNA knockdown of collagen-binding integrins on human umbilical vein endothelial cell migration and tube formation Scientific Reports, 2022-12-14;12(1):21601. 2022-12-14 [PMID: 36517525] (Cell Culture, Human)
• C Cegarra, B Cameron, C Chaves, T Dabdoubi, TM Do, B Genêt, V Roudières, Y Shi, P Tchepikoff, D Lesuisse An innovative strategy to identify new targets for delivering antibodies to the brain has led to the exploration of the integrin family PLoS ONE, 2022-09-15;17(9):e0274667. 2022-09-15 [PMID: 36108060] (ELISA Capture, Human)
• KH Koh, Y Cao, S Mangos, NJ Tardi, RR Dande, HW Lee, B Samelko, MM Altintas, VP Schmitz, H Lee, K Mukherjee, V Peev, DJ Cimbaluk, J Reiser, E Hahm Nonimmune cell-derived ICOS ligand functions as a renoprotective alphavbeta3 integrin-selective antagonist J. Clin. Invest., 2019-03-18;129(4):1713-1726. 2019-03-18 [PMID: 30747722] (Surface Plasmon Resonance (SPR, Human)
• Haidari M, Zhang W, Caivano A, Chen Z, Ganjehei L, Mortazavi A, Stroud C, Woodside D, Willerson J, Dixon R Integrin alpha2beta1 mediates tyrosine phosphorylation of vascular endothelial cadherin induced by invasive breast cancer cells. J Biol Chem, 2012-07-25;287(39):32981-92. 2012-07-25 [PMID: 22833667] (Bioassay, Human)

Bioinformatics

• Gene Symbol: ITGA3
• Uniprot:
  • P26006 (Integrin alpha 3) & P05556 (Integrin beta 1)()