Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)


There are currently no images for CXCL8/IL-8 (618-IL).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) Summary

Lyophilized from a 0.2 um filtered solution in PBS with BSA as a carrier protein.
Details of Functionality
Measured by its ability to induce myeloperoxidase release from cytochalasin B-treated human neutrophils. Schröder, J.M. et al. (1987) J. Immunol. 139:3474. The ED50 for this effect is 0.2-0.4 µg/mL. Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 1-5 ng/mL.
E. coli-derived human IL-8/CXCL8 protein
Ala23-Ser99 & Ser28-Ser99
Accession #
N-terminal Sequence
Ala23 & Ser28
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


Theoretical MW
8.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
618-IL in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived)

  • 3-10C
  • AMCF-I
  • C-X-C motif chemokine 8
  • CXCL8
  • Emoctakin
  • GCP1
  • GCP-1TSG-1
  • IL8
  • IL-8
  • interleukin 8
  • K60
  • LAI
  • LECT
  • LUCT
  • member 8
  • NAF
  • NAP1
  • NAP-1NAP1
  • NCF
  • Neutrophil-activating protein 1
  • Protein 3-10C
  • T cell chemotactic factor
  • T-cell chemotactic factor
  • TCF
  • TSG1


Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine CXCL8 (4). There is no CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of CXCL8 is regulated by these truncations, by CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents CXCL8 interaction with CXCR1 (11). CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).
  1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
  2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
  3. Pichert, A. et al. (2012) Biomatter 2:142.
  4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
  5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
  6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
  7. Neote, K. et al. (1994) Blood 84:44.
  8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
  9. Gerszten, R.E. et al. (1999) Nature 398:718.
  10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
  11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
  12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
  13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
  14. Li, A. et al. (2005) Angiogenesis 8:63.
  15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
  16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Rt, Rb
Applications: WB, Flow, IHC, IHC-P, KO
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Po, Bv, Ma
Applications: WB, ChIP, DB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, B/N, CyTOF-ready, Dual ISH-IHC, ELISA(Cap), Flow-CS, Flow-IC, KD, KO
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Species: Hu
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, DB, ELISA, Flow, Func, ICC/IF, IP, In vitro, B/N, Flow-CS

Publications for CXCL8/IL-8 (618-IL)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: Binding Assay, Bioassay.

Filter By Application
Binding Assay
All Applications
Filter By Species
All Species
Showing Publications 1 - 5 of 5.
Publications using 618-IL Applications Species
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Brown CE, Vishwanath RP, Aguilar B, Starr R, Najbauer J, Aboody KS, Jensen MC Tumor-derived chemokine MCP-1/CCL2 is sufficient for mediating tumor tropism of adoptively transferred T cells. J. Immunol., 2007;179(5):3332-41. 2007 [PMID: 17709550] (Bioassay, Human) Bioassay Human
Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression. Int. J. Oncol., 2007;30(4):977-83. 2007 [PMID: 17332938] (Bioassay, Human) Bioassay Human
Fan X, Patera AC, Pong-Kennedy A, Deno G, Gonsiorek W, Manfra DJ, Vassileva G, Zeng M, Jackson C, Sullivan L, Sharif-Rodriguez W, Opdenakker G, Van Damme J, Hedrick JA, Lundell D, Lira SA, Hipkin RW Murine CXCR1 is a functional receptor for GCP-2/CXCL6 and interleukin-8/CXCL8. J. Biol. Chem., 2007;282(16):11658-66. 2007 [PMID: 17197447] (Binding Assay, Mouse) Binding Assay Mouse
Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. J. Cell. Physiol., 2007;210(2):417-27. 2007 [PMID: 17096385] (Bioassay, Human) Bioassay Human

Reviews for CXCL8/IL-8 (618-IL) (0)

There are no reviews for CXCL8/IL-8 (618-IL). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for CXCL8/IL-8 (618-IL) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional CXCL8/IL-8 Products

Bioinformatics Tool for CXCL8/IL-8 (618-IL)

Discover related pathways, diseases and genes to CXCL8/IL-8 (618-IL). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CXCL8/IL-8 (618-IL)

Discover more about diseases related to CXCL8/IL-8 (618-IL).

Pathways for CXCL8/IL-8 (618-IL)

View related products by pathway.

PTMs for CXCL8/IL-8 (618-IL)

Learn more about PTMs related to CXCL8/IL-8 (618-IL).

Blogs on CXCL8/IL-8

There are no specific blogs for CXCL8/IL-8, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-8/CXCL8 (Endothelial Cell Derived) and receive a gift card or discount.


Gene Symbol IL8