Recombinant Human IL-18/IL-1F4 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-18/IL-1F4 Protein Summary

Additional Information
A New rh IL-18 is Now Available! Competitively priced; Higher purity; Lower endotoxin; Manufactured in-house!
Details of Functionality
Induction of IFN-gamma by KG-1 cell [human myelomonocyte; ATCC CCL246] in response to the recombinant human IL-18 was measured using human IFN-gamma ELISA. The activity of lot 111 was as follows: IL-18 final concentration 10 ng/mL; IFN-gamma induction 48.1 IU/mL and IL-18 final concentration 20 ng/mL; IFN-gamma induction 64.3 IU/mL.
Source
E. coli-derived human IL-18/IL-1F4 protein
37-193 aa
Protein/Peptide Type
Recombinant Proteins
Gene
IL18
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
B001-5 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
25 μg in PBS containing 0.1% BSA and 1% sucrose.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Reconstitution Instructions
Reconstitute in 250 μL of ice-cold distilled water on ice.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-18/IL-1F4 Protein

  • Iboctadekin
  • IFN-gamma-inducing factor
  • IGIF
  • IGIFIL-1 gamma
  • IL18
  • IL-18
  • IL-18MGC12320
  • IL-1F4
  • IL1F4iboctadekin
  • IL-1g
  • Interferon gamma-inducing factor
  • interleukin 18 (interferon-gamma-inducing factor)
  • Interleukin-1 gamma
  • interleukin-18

Background

Interleukin-18 (IL-18) is a proinflammatory cytokine in the IL-1 family that exerts distinct immune effects depending on the local cytokine environment. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. The precursor is activated intracellularly by Caspase-1 mediated proteolysis to release the 17 kDa mature cytokine. The precursor can also be released by necrotic cells for extracellular cleavage by multiple proteases.
IL-18 activation is induced by infection or tissue damage and contributes to disease pathology in chronic inflammation (1-3). IL-18 binds to the widely expressed IL-18 R alpha which recruits IL-18 R beta to form the signaling receptor complex (4, 5). Its bioactivity is negatively regulated by interactions with IL-18 binding proteins and virally encoded IL-18BP homologs (6). In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells (7, 8). In the absence of IL-12 or IL-15, however, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils (9, 10). In the presence of IL-1 beta or IL-23, IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells (11). IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst (12, 13). In cancer, IL-18 exhibits diverse activities including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis (14). Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18 (15). Alternative splicing in human ovarian cancer generates an isoform that is resistant to Caspase-1 activation (16). A cell surface form can be expressed on M-CSF induced macrophages and released in response to bacterial endotoxin (17).
  1. Dinarello, C.A. et al. (2013) Front. Immunol. 4:289.
  2. Smith, D.E. (2011) J. Leukoc. Biol. 89:383.
  3. Gu, Y. et al. (1997) Science 275:206.
  4. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
  5. Cheung, H. et al. (2005) J. Immunol. 174:5351.
  6. Novick, D. et al. (1999) Immunity 10:127.
  7. Fehniger, T.A. et al. (1999) J. Immunol. 162:4511.
  8. Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
  9. Yoshimoto, T. et al. (2000) Nat. Immunol. 1:132.
  10. Kroeger, K.M. et al. (2009) J. Leukoc. Biol. 86:769.
  11. Lalor, S.J. et al. (2011) J. Immunol. 186:5738.
  12. Li, J. et al. (2004) Cell. Immunol. 227:103.
  13. Elbim, C. et al. (2005) Clin. Diagn. Lab. Immunol. 12:436.
  14. Fabbi, M. et al. (2015) J. Leukoc. Biol. 97:665.
  15. Ushio, S. et al. (1996) J. Immunol. 156:4274.
  16. Gaggero, A. et al. (2004) Oncogene 23:7552.
  17. Bellora, F. et al. (2012) Eur. J. Immunol. 42:1618.

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Publications for IL-18/IL-1F4 (B001-5)(39)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), ELISA Developmet.


Filter By Application
Bioassay
(37)
ELISA (Standard)
(1)
ELISA Developmet
(1)
All Applications
Filter By Species
Human
(38)
N/A
(1)
All Species
Showing Publications 1 - 10 of 39. Show All 39 Publications.
Publications using B001-5 Applications Species
S Suliman, M Murphy, M Musvosvi, A Gela, EW Meermeier, H Geldenhuys, C Hopley, A Toefy, N Bilek, A Veldsman, WA Hanekom, JL Johnson, WH Boom, G Obermoser, H Huang, M Hatherill, DM Lewinsohn, E Nemes, TJ Scriba MR1-Independent Activation of Human Mucosal-Associated Invariant T Cells by Mycobacteria J. Immunol., 2019;0(0):. 2019 [PMID: 31611259] (Bioassay, Human) Bioassay Human
T Hofland, I de Weerdt, H Ter Burg, R de Boer, S Tannheimer, SH Tonino, AP Kater, E Eldering Dissection of the Effects of JAK and BTK Inhibitors on the Functionality of Healthy and Malignant Lymphocytes J. Immunol., 2019;0(0):. 2019 [PMID: 31511358] (Bioassay, Human) Bioassay Human
RP Semper, M Vieth, M Gerhard, R Mejías-Luq Helicobacter pylori Exploits the NLRC4 Inflammasome to Dampen Host Defenses J. Immunol., 2019;0(0):. 2019 [PMID: 31511355] (Bioassay, Human) Bioassay Human
CL Zimmer, M Cornillet, C Solà-Riera, KW Cheung, MA Ivarsson, MQ Lim, N Marquardt, YS Leo, DC Lye, J Klingström, PA MacAry, HG Ljunggren, L Rivino, NK Björkström NK cells are activated and primed for skin-homing during acute dengue virus infection in humans Nat Commun, 2019;10(1):3897. 2019 [PMID: 31467285] (Bioassay, Human) Bioassay Human
TA Mace, MB Ware, SA King, S Loftus, MR Farren, E McMichael, S Scoville, C Geraghty, G Young, WE Carson, SK Clinton, GB Lesinski Soy isoflavones and their metabolites modulate cytokine-induced natural killer cell function Sci Rep, 2019;9(1):5068. 2019 [PMID: 30911044] (Bioassay, Human) Bioassay Human
LD Uppendahl, M Felices, L Bendzick, C Ryan, B Kodal, P Hinderlie, KLM Boylan, APN Skubitz, JS Miller, MA Geller Cytokine-induced memory-like natural killer cells have enhanced function, proliferation, and in vivo expansion against ovarian cancer cells Gynecol. Oncol., 2019;0(0):. 2019 [PMID: 30658847] (Bioassay, Human) Bioassay Human
G Han, MH Spitzer, SC Bendall, WJ Fantl, GP Nolan Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry Nat Protoc, 2018;0(0):. 2018 [PMID: 30258176] (Bioassay, Human) Bioassay Human
L Golden-Mas, RH McMahan, MS Kriss, AL Kilgore, L Cheng, RJ Dran, A Wieland, HR Rosen Early and late changes in natural killer cells in response to ledipasvir/sofosbuvir treatment Hepatol Commun, 2018;2(4):364-375. 2018 [PMID: 29619416] (Bioassay, Human) Bioassay Human
D Peppa, I Pedroza-Pa, P Pellegrino, I Williams, MK Maini, P Borrow Adaptive Reconfiguration of Natural Killer Cells in HIV-1 Infection Front Immunol, 2018;9(0):474. 2018 [PMID: 29616021] (Bioassay, Human) Bioassay Human
L van den Dr, MAA Claassen, ZMA Groothuism, E van Gorp, A Boonstra Immune activation in prolonged cART-suppressed HIV patients is comparable to that of healthy controls Virology, 2017;509(0):133-139. 2017 [PMID: 28644978] (Bioassay, Human) Bioassay Human
Show All 39 Publications.

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Gene Symbol IL18
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