Recombinant Human IL-17B (CHO) Protein, CF

Recombinant Human IL-17B induces IL-8 secretion by HepG2 human hepatocellular carcinoma cells.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-17B (CHO) Protein, CF Summary

• Details of Functionality: Measured by its ability to induce IL-8 secretion by HepG2 human hepatocellular carcinoma cells. Tang, Y. et al. (2011) Clin. Exp. Immunol. 166:281. The ED₅₀ for this effect is 0.4-2 μg/mL.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-17B protein
  Gln21-Phe180
• Accession #: Q9UHF5
• N-terminal Sequence: Gln21, Ser51 & Gly102
  Gln21 predicted, sequencing might be blocked
• Structure / Form: Noncovalently-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL17B
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 18 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 20-25 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in Sodium Phosphate and NaCl.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-17B (CHO) Protein, CF

• Cytokine Zcyto7
• cytokine-like protein ZCYTO7
• IL17B
• IL-17B
• IL-17Binterleukin-17 beta
• IL20
• IL-20interleukin-17B
• interleukin 17B
• interleukin 20
• Interleukin-20
• MGC138900
• MGC138901
• neuronal interleukin-17 related factor
• Neuronal interleukin-17-related factor
• NIRF
• ZCYTO7interleukin-20

Background

Interleukin-17B (IL-17B), is an approximately 20 kDa glycosylated cytokine that plays a role in inflammation and bone growth. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17B shares 90% and 91% amino acid sequence identity with mouse and rat IL-17B, respectively (3, 4). IL-17B binds to IL-17 RB and induces the production of inflammatory cytokines and the infiltration of inflammatory immune cells (3, 4). IL-17B is expressed in the central nervous system, testis, ovary, stomach, pancreas, and small intestine (3-5). In the skeletal system, IL-17B is expressed in bone collars surrounding primary ossification centers as well as in epiphyseal plate chondrocytes during development and after bone fracture (6, 7). IL-17B is up-regulated in arthritic cartilage where it exacerbates the severity of disease (8). It is also up-regulated in aggressive breast cancer cell lines, and its exaggerated signaling through IL-17 RB promotes tumorigenicity (9). It inhibits the adhesion of vascular endothelial cells to Matrigel and enhances their migration (10).

1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
4. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.
5. Moore, E.E. et al. (2002) Neuromuscul. Disord. 12:141.
6. Kokubu, T. et al. (2008) J. Histochem. Cytochem. 56:89.
7. You, Z. et al. (2005) Biochem. Biophys. Res. Commun. 326:624.
8. Yamaguchi, Y. et al. (2007) J. Immunol. 179:7128.
9. Huang, C.-K. et al. (2013) Oncogene July 15 Epub.
10. Sanders, A.J. et al. (2010) J. Oncol. 2010:817375.

Bioinformatics

• Gene Symbol: IL17B
• Uniprot:
  • Q9UHF5()