Recombinant Human IL-13 Protein

Images

 
There are currently no images for IL-13 (213-ILB).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations

Recombinant Human IL-13 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human IL-13 protein
Gly21-Asn132
Accession #
N-terminal Sequence
Gly21
Protein/Peptide Type
Recombinant Proteins
Gene
IL13
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
213-ILB in the following application:

Publications
Read Publications using
213-ILB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-13 Protein

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells (1‑3). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (4). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑regulation of CD23/Fc epsilon RII (1, 5). IL-13 binds with low affinity to the transmembrane IL-13 R alpha 1 which then forms a signaling complex with the transmembrane IL-4 R alpha (6‑8). This high affinity receptor complex also functions as the type 2 IL-4 receptor (6, 7). IL‑13 R alpha 2 inhibits responses to both IL-13 and IL-4. It binds IL-13 with high affinity (9, 10) and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex (11, 12). It also blocks signaling through IL-4-occupied IL-13 R alpha 1/IL-4 R alpha receptor complexes (12, 13). In addition, IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis (14‑16).
  1. Saggini, A. et al. (2011) Int. J. Immunopathol. Pharmacol. 24:305.
  2. Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.
  3. Liang, H.E. et al. (2012) Nat. Immunol. 13:58.
  4. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  5. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  6. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Chen, W. et al. (2009) J. Immunol. 183:7870.
  10. Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.
  11. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  12. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  13. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  14. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
  15. Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.
  16. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

Customers Who Viewed This Item Also Viewed...

NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP
NB600-922
Species: Ch
Applications: WB, ELISA, IHC, IHC-P, IP
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
AF152
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7954-GM/CF
Species: Hu
409-ML/CF
Species: Mu
NB7343
Species: Ca
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89146
Species: Hu
Applications: ICC/IF, IHC, IHC-P
213-ILB
Species: Hu
Applications: Bioactivity

Publications for IL-13 (213-ILB)(64)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 3 applications: Bioassay, ELISA (Standard), Neutralization.


Filter By Application
Bioassay
(62)
ELISA (Standard)
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(62)
N/A
(1)
All Species
Showing Publications 1 - 10 of 64. Show All 64 Publications.
Publications using 213-ILB Applications Species
G Cantero-Re, CM Butnaru, N Brouwers, S Mitrovic, MA Valverde, V Malhotra Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of Ca2+-induced mucin secretion from goblet cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30482841] (Bioassay, Human) Bioassay Human
L She, Y Qin, J Wang, C Liu, G Zhu, G Li, M Wei, C Chen, G Liu, D Zhang, X Chen, Y Wang, Y Qiu, Y Tian, X Zhang, Y Liu, D Huang Tumor-associated macrophages derived CCL18 promotes metastasis in squamous cell carcinoma of the head and neck Cancer Cell Int., 2018;18(0):120. 2018 [PMID: 30181713] (Bioassay, Human) Bioassay Human
SR Kim, JM Lewis, BM Cyrenne, PF Monico, FN Mirza, KR Carlson, FM Foss, M Girardi BET inhibition in advanced cutaneous T cell lymphoma is synergistically potentiated by BCL2 inhibition or HDAC inhibition Oncotarget, 2018;9(49):29193-29207. 2018 [PMID: 30018745] (Bioassay, Human) Bioassay Human
G Genard, AC Wera, C Huart, B Le Calve, S Penninckx, A Fattacciol, T Tabarrant, C Demazy, N Ninane, AC Heuskin, S Lucas, C Michiels Proton irradiation orchestrates macrophage reprogramming through NF?B signaling Cell Death Dis, 2018;9(7):728. 2018 [PMID: 29950610] (Bioassay, Human) Bioassay Human
Y Ito, R Al Mubarak, N Roberts, K Correll, W Janssen, J Finigan, R Mishra, HW Chu IL-13 induces periostin and eotaxin expression in human primary alveolar epithelial cells: Comparison with paired airway epithelial cells PLoS ONE, 2018;13(4):e0196256. 2018 [PMID: 29672593] (Bioassay, Human) Bioassay Human
EY Huang, JC Chang, HH Chen, CY Hsu, HC Hsu, KL Wu Carcinoembryonic antigen as a marker of radioresistance in colorectal cancer: a potential role of macrophages BMC Cancer, 2018;18(1):321. 2018 [PMID: 29580202] (Bioassay, Human) Bioassay Human
E Berdyshev, E Goleva, I Bronova, N Dyjack, C Rios, J Jung, P Taylor, M Jeong, CF Hall, BN Richers, KA Norquest, T Zheng, MA Seibold, DY Leung Lipid abnormalities in atopic skin are driven by type 2 cytokines JCI Insight, 2018;3(4):. 2018 [PMID: 29467325] (Bioassay, Human) Bioassay Human
R Bruen, S Curley, S Kajani, D Crean, ME O'Reilly, MB Lucitt, CG Godson, FC McGillicud, O Belton Liraglutide dictates macrophage phenotype in apolipoprotein E null mice during early atherosclerosis Cardiovasc Diabetol, 2017;16(1):143. 2017 [PMID: 29110715] (Bioassay, Human) Bioassay Human
Y Wang, JB Mumm, R Herbst, R Kolbeck, Y Wang IL-22 Increases Permeability of Intestinal Epithelial Tight Junctions by Enhancing Claudin-2 Expression J. Immunol., 2017;0(0):. 2017 [PMID: 28939759] (Bioassay, Human) Bioassay Human
J Zeitvogel, N Jokmin, S Rieker, I Klug, C Brandenber, T Werfel GATA3 regulates FLG and FLG2 expression in human primary keratinocytes Sci Rep, 2017;7(1):11847. 2017 [PMID: 28928464] (Bioassay, Human) Bioassay Human
Show All 64 Publications.

Reviews for IL-13 (213-ILB) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 213-ILB:
Filter by Applications
CellProlif
(1)
All Applications
Filter by Species
All Species
Images Ratings Applications Species Date Details
 IL-13 213-ILB
Enlarge
reviewed by:
Anonymous
06/09/2019
View

Summary

LotDCSK0518041
CommentsCells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
Cell Proliferation IL-13 213-ILB
Enlarge
reviewed by:
Anonymous
CellProlif 10/07/2018
View

Summary

ApplicationCell Proliferation
LotDCSK0518041

Comments

CommentsCells were supplied with the cytokinetreatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-13 to differentiate DC cells

FAQs for IL-13 (213-ILB) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IL-13 Products

Bioinformatics Tool for IL-13 (213-ILB)

Discover related pathways, diseases and genes to IL-13 (213-ILB). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-13 (213-ILB)

Discover more about diseases related to IL-13 (213-ILB).
 

Pathways for IL-13 (213-ILB)

View related products by pathway.

PTMs for IL-13 (213-ILB)

Learn more about PTMs related to IL-13 (213-ILB).

Blogs on IL-13.

FLICE, FLICE, baby
Cell death via apoptosis is a fundamental cellular function triggered by the cell death receptor family and their ligands which signal through downstream adaptor molecules and the caspase protease family. All caspases exist in a precursor form compose...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
06/09/2019
Application:
Species:

Anonymous
10/07/2018
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL13
Uniprot