Recombinant Human IL-13 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-13 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.5-2.5 ng/mL.
Source
E. coli-derived human IL-13 protein
Gly21-Asn132
Accession #
N-terminal Sequence
Gly21
Protein/Peptide Type
Recombinant Proteins
Gene
IL13
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
12.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
9 kDa, reducing conditions
Reviewed Applications
Read 2 Reviews rated 5
using
213-ILB in the following application:

Publications
Read Publications using
213-ILB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-13 Protein

  • ALRHMGC116789
  • BHR1interleukin-13
  • IL13
  • IL-13
  • IL-13MGC116788
  • interleukin 13
  • MGC116786
  • NC30
  • P600

Background

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells (1‑3). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (4). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑regulation of CD23/Fc epsilon RII (1, 5). IL-13 binds with low affinity to the transmembrane IL-13 R alpha 1 which then forms a signaling complex with the transmembrane IL-4 R alpha (6‑8). This high affinity receptor complex also functions as the type 2 IL-4 receptor (6, 7). IL‑13 R alpha 2 inhibits responses to both IL-13 and IL-4. It binds IL-13 with high affinity (9, 10) and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex (11, 12). It also blocks signaling through IL-4-occupied IL-13 R alpha 1/IL-4 R alpha receptor complexes (12, 13). In addition, IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis (14‑16).
  1. Saggini, A. et al. (2011) Int. J. Immunopathol. Pharmacol. 24:305.
  2. Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.
  3. Liang, H.E. et al. (2012) Nat. Immunol. 13:58.
  4. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  5. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  6. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Chen, W. et al. (2009) J. Immunol. 183:7870.
  10. Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.
  11. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  12. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  13. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  14. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
  15. Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.
  16. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

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Publications for IL-13 (213-ILB)(76)

We have publications tested in 2 confirmed species: Human, N/A.

We have publications tested in 5 applications: Bioassay, Cell Culture, Differentiation, ELISA (Standard), Neutralization.


Filter By Application
Bioassay
(68)
Cell Culture
(5)
Differentiation
(1)
ELISA (Standard)
(1)
Neutralization
(1)
All Applications
Filter By Species
Human
(74)
N/A
(1)
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Showing Publications 1 - 10 of 76. Show All 76 Publications.
Publications using 213-ILB Applications Species
V Delprat, C Tellier, C Demazy, M Raes, O Feron, C Michiels Cycling hypoxia promotes a pro-inflammatory phenotype in macrophages via JNK/p65 signaling pathway Sci Rep, 2020;10(1):882. 2020 [PMID: 31964911] (Bioassay, Human) Bioassay Human
G Liu, L Yin, X Ouyang, K Zeng, Y Xiao, Y Li M2 Macrophages Promote HCC Cells Invasion and Migration via miR-149-5p/MMP9 Signaling J Cancer, 2020;11(5):1277-1287. 2020 [PMID: 31956374] (Cell Culture, Human) Cell Culture Human
SC Lee, MS Tang, AV Easton, JC Devlin, LL Chua, I Cho, FM Moy, TF Khang, YAL Lim, P Loke Linking the effects of helminth infection, diet and the gut microbiota with human whole-blood signatures PLoS Pathog., 2019;15(12):e1008066. 2019 [PMID: 31841569] (Cell Culture, Human) Cell Culture Human
SD Pauls, LA Rodway, T Winter, CG Taylor, P Zahradka, HM Aukema Alpha-linolenic acid enhances the phagocytic and secretory functions of alternatively activated macrophages in part via changes to the oxylipin profile Int. J. Biochem. Cell Biol., 2019;119(0):105662. 2019 [PMID: 31811954] (Bioassay, Human) Bioassay Human
N Berges, MS Nawaz, T Børresdatt, L Hagen, M Bjørås, JK Laerdahl, I Alseth Complex alternative splicing of human Endonuclease V mRNA, but evidence for only a single protein isoform PLoS ONE, 2019;14(11):e0225081. 2019 [PMID: 31703097] (Cell Culture, Human) Cell Culture Human
X Unamuno, J Gómez-Ambr, B Ramírez, A Rodríguez, S Becerril, V Valentí, R Moncada, C Silva, J Salvador, G Frühbeck, V Catalán NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling Cell. Mol. Immunol., 2019;0(0):. 2019 [PMID: 31551515] (Bioassay, Human) Bioassay Human
Y Wu, MM Konaté, J Lu, H Makhlouf, R Chuaqui, S Antony, JL Meitzler, MJ Difilippan, H Liu, A Juhasz, G Jiang, I Dahan, K Roy, JH Doroshow IL-4 and IL-17A Cooperatively Promote Hydrogen Peroxide Production, Oxidative DNA Damage, and Upregulation of Dual Oxidase 2 in Human Colon and Pancreatic Cancer Cells J. Immunol., 2019;0(0):. 2019 [PMID: 31548328] (Bioassay, Human) Bioassay Human
S Sugihara, S Sugimoto, K Tachibana, M Kobashi, H Nomura, T Miyake, Y Hirai, O Yamasaki, S Morizane TNF-α and IL-17A induce the expression of lympho-epithelial Kazal-type inhibitor in epidermal keratinocytes J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 31501002] (Cell Culture, Human) Cell Culture Human
X Qin, M Liu, S Zhang, C Wang, T Zhang The role of IL-36? and its regulation in eosinophilic inflammation in allergic rhinitis Cytokine, 2019;117(0):84-90. 2019 [PMID: 30831444] (Bioassay, Human) Bioassay Human
G Cantero-Re, CM Butnaru, N Brouwers, S Mitrovic, MA Valverde, V Malhotra Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of Ca2+-induced mucin secretion from goblet cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 30482841] (Bioassay, Human) Bioassay Human
Show All 76 Publications.

Reviews for IL-13 (213-ILB) (2) 52

Average Rating: 5
(Based on 2 reviews)

Reviews using 213-ILB:
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CellProlif
(1)
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 IL-13 213-ILB
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reviewed by:
Anonymous
06/09/2019
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Summary

LotDCSK0518041
CommentsCells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
Cell Proliferation IL-13 213-ILB
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reviewed by:
Anonymous
CellProlif 10/07/2018
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Summary

ApplicationCell Proliferation
LotDCSK0518041

Comments

CommentsCells were supplied with the cytokinetreatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-13 to differentiate DC cells

FAQs for IL-13 (213-ILB) (0)

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Recent Reviews

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Anonymous
06/09/2019
Application:
Species:

Anonymous
10/07/2018
Application: CellProlif
Species:

Bioinformatics

Gene Symbol IL13
Uniprot