Recombinant Human IL-11 R alpha Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-11 R alpha Protein, CF Summary

• Details of Functionality: Measured by its ability to bind recombinant human IL-11 in a functional ELISA with an estimated K_d <1 nM.
• Source: Chinese Hamster Ovary cell line, CHO-derived human IL-11 R alpha protein
  Ser24-Val363, with a C-terminal 6-His tag
• Accession #: Q14626
• N-terminal Sequence: Ser24 & Cys26
• Protein/Peptide Type: Recombinant Proteins
• Gene: IL11RA
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 40-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 500 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-11 R alpha Protein, CF

• AI314697
• GP130
• IL-11 R alpha
• IL11R alpha
• IL11RA
• IL-11Ra
• Il11ra2
• NR1

Background

Interleukin-11 receptor alpha (IL-11 R alpha ) is a 49 kDa type I transmembrane member of the gp130 subfamily of the hematopoietic cytokine receptor family (1-3). Mature human IL-11 R alpha consists of a 347 amino acid (aa) extracellular domain (ECD) that contains a C2 type Ig-like domain, two fibronectin type III domains, two potential glycosylation sites, and a WSxWS motif, followed by a 21 aa transmembrane region and a 31 aa cytoplasmic domain (4). Within the ECD, human IL-11 R alpha shares 84%, 82%, 90%, and 86% aa sequence identity with mouse, rat, equine, and bovine IL-11 R alpha , respectively. Alternative splicing generates an additional isoform that lacks the cytoplasmic domain (4). Upon low affinity binding to IL-11, IL-11 R alpha associates with gp130 to form a high affinity receptor complex (1, 3). gp130 also functions as a subunit in the receptors for Cardiotrophin-1, CNTF, IL-6, IL-27, IL-31, LIF, and Oncostatin M (5). IL-11 R alpha is widely expressed in adults, embryos, and embryonic stem cells (4, 6, 7). Deletion of IL-11 R alpha in female mice causes faulty decidualization, lack of decidual NK cells, and infertility (8-10). IL-11 is anti-apoptotic for oligodendrocytes, and lack of IL-11 R alpha increases the severity of experimental autoimmune encephalitis (11, 12). IL-11 R alpha is also anti-apoptotic for colonic epithelia, and increased IL-11 signaling may be a factor in inflammation-associated gastrointestinal cancer development (3, 13). IL-11 R alpha enhances osteoclast differentiation and bone remodeling but inhibits adipocyte differentiation (1, 2). Recombinant soluble IL-11 R alpha confers IL-11 responsiveness to cells expressing gp130, while in cells expressing transmembrane IL-11 R alpha and gp130, soluble IL-11 R alpha acts as an IL-11 antagonist (14-16).

1. Sims, N.A. and N.C. Walsh (2010) BMB Reports 43:513.
2. White, U.A. and J.M. Stephens (2011) Curr. Pharm. Des. 17:340.
3. Kiessling, S. et al. (2004) J. Biol. Chem. 279:10304.
4. Cherel, M. et al. (1995) Blood 86:2534.
5. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85.
6. Hilton, D.J. et al. (1994) EMBO J. 13:4765.
7. Davidson, A.J. et al. (1997) Stem Cells 15:119.
8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
9. Robb, L. et al. (1998) Nat. Med. 4:303.
10. Ain, R. et al. (2004) Dev. Dyn. 231:700.
11. Gurfein, B.T. et al. (2009) J. Immunol. 183:4229.
12. Zhang, J. et al. (2011) J. Immunol. 187:1129.
13. Putoczki, T. and M. Ernst (2010) J. Leukoc. Biol. 88:1109.
14. Curtis, D.J. et al. (1997) Blood 90:4403.
15. Baumann, H. et al. (1996) J. Immunol. 157:284.
16. Karow, J. et al. (1996) Biochem. J. 318:489.

Publications for IL-11R alpha (8895-MR)(4)

Publications using 8895-MR

• Liang, P;Ness, J;Rapp, J;Boneva, S;Schwämmle, M;Jung, M;Schlunck, G;Agostini, H;Bucher, F; Characterization of the angiomodulatory effects of Interleukin 11 cis- and trans-signaling in the retina Journal of neuroinflammation 2024-09-18 [PMID: 39294742] (Bioassay, Human)
• M Lindkvist, MM Zegeye, M Grenegård, LU Ljungberg Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 2022-01-27;23(3):. 2022-01-27 [PMID: 35163371] (Bioassay, Human)
• J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH Nature Communications, 2021-01-04;12(1):66. 2021-01-04 [PMID: 33397952] (Bioassay, Human)
• P Aryappalli, K Shabbiri, RJ Masad, RH Al-Marri, SM Haneefa, YA Mohamed, K Arafat, S Attoub, O Cabral-Mar, KB Ramadi, MJ Fernandez-, BK Al-Ramadi Inhibition of Tyrosine-Phosphorylated STAT3 in Human Breast and Lung Cancer Cells by Manuka Honey is Mediated by Selective Antagonism of the IL-6 Receptor Int J Mol Sci, 2019-09-05;20(18):. 2019-09-05 [PMID: 31491838] (Bioassay, Insect)

Bioinformatics

• Gene Symbol: IL11RA
• Uniprot:
  • Q14626()