Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein Summary

• Additional Information: Intended for preclinical researchers who may transition to GMP IL-1beta for their clinical work
• Details of Functionality: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED₅₀ for this effect is <12 pg/mL.
  The specific activity of Recombinant Human IL-1 beta is >5.0 x 10⁷ IU/mg, which is calibrated against recombinant human IL-1 beta WHO International Standard (NIBSC code: 86/680).
• Source: E. coli-derived human IL-1 beta/IL-1F2 protein
  Ala117-Ser269
  Produced using non-animal reagents in an animal-free laboratory.
• Accession #: NP_000567.1
• N-terminal Sequence: Ala117 & Pro118
• Protein/Peptide Type: Animal-Free Recombinant Proteins
• Gene: IL1B
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 17 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 12 months, 2 to 8 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is covered by one or more of the following U.S. patents: 5,681,933 and 5,474,899.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 beta/IL-1F2, Animal-Free Protein

• catabolin
• IL1 beta
• IL-1 beta
• IL-1
• IL1B
• IL-1b
• IL1-BETA
• IL-1F2
• IL1F2IL-1 beta
• interleukin 1, beta
• interleukin-1 beta
• preinterleukin 1 beta
• pro-interleukin-1-beta

Background

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL‑1 R3/IL‑1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1‑4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5‑7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%‑78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL‑1 beta .

1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
5. March, C.J. et al. (1985) Nature 315:641.
6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Manufacturing Process

Animal-Free Manufacturing Conditions
Our dedicated controlled-access animal-free laboratories ensure that at no point in production are the products exposed to potential contamination by animal components or byproducts. Every stage of manufacturing is conducted in compliance with R&D Systems' stringent Standard Operating Procedures (SOPs). Production and purification procedures use equipment and media that are confirmed animal-free.

Production

• All molecular biology procedures use animal-free media and dedicated labware.
• Dedicated fermentors are utilized in committed animal-free areas.

Purification

• Protein purification columns are animal-free.
• Bulk proteins are filtered using animal-free filters.
• Purified proteins are stored in animal-free containers in a dedicated cold storage room.

Quality Assurance

• Low Endotoxin Level.
• No impairment of biological activity.
• High quality product obtained under stringent conditions.
• For ex vivo research or bioproduction, additional documentation can be provided.

Please read our complete Animal-Free Statement

 

Publications for IL-1 beta/IL-1F2 (AFL201)(1)

Publication using AFL201

• Souheil-Antoine Younes IL-15 promotes activation and expansion of CD8+ T cells in HIV-1 infection J Clin Invest, 2016-06-20;126(7):2745-2756. 2016-06-20 [PMID: 27322062] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IL1B
• Uniprot:
  • NP_000567.1()