Recombinant Human IGSF4A/SynCAM1 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human IGSF4A/SynCAM1 Protein, CF Summary

• Details of Functionality: Measured by its binding activity in a functional ELISA. Galiberet, L. et al. (2005) J. Biol. Chem. 280:21955. Immobilized Recombinant Human CRTAM Fc Chimera (Catalog # 1695-CR) at 2 µg/mL (100 µL/well) can bind Recombinant Human IGSF4A/SynCAM1 with a linear range of 3-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human IGSF4A/SynCAM1 protein
  Ile39-Ala375, with a C-terminal 6-His tag
• Accession #: Q9BY67
• N-terminal Sequence: Ile39
• Protein/Peptide Type: Recombinant Proteins
• Gene: CADM1
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 38.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 70-80 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGSF4A/SynCAM1 Protein, CF

• BL2
• Cadm1
• cell adhesion molecule 1
• IgSF4
• IGSF4A
• IGSF4ADKFZp686F1789
• IGSF4immunoglobulin superfamily, member 4D variant 2
• Immunoglobulin superfamily member 4
• immunoglobulin superfamily, member 4
• Necl-2
• NECL2MGC149785
• nectin-like 2
• Nectin-like protein 2
• RA175
• sgIGSF
• Spermatogenic immunoglobulin superfamily
• ST17
• sTSLC-1
• Synaptic cell adhesion molecule
• SynCAM
• SynCAM1
• SYNCAMMGC51880
• TSLC1
• TSLC-1
• TSLC1/Nectin-like 2/IGSF4
• Tumor suppressor in lung cancer 1SYNCAM1

Background

IGSF4A is a 75 kDa immunoglobulin superfamily protein that mediates calcium-independent adhesive interactions, either homophilic or heterophilic with CRTAM (class I-restricted T cell-associated molecule), Necl-1 (SynCAM3) or Nectin-3 (1, 4, 5). Alternate names for IGSF4A reflect its activities. As TSLC-1 (tumor suppressor in lung cancer-1), it is downregulated in non-small cell lung cancer (2). This may allow evasion of antitumor responses, since TSLC-1 interaction with CRTAM on activated NK and CD8⁺ T cells stimulates cytotoxicity and upregulates IFN-gamma production (3, 4). As Necl-2 (nectin-like 2), it is expressed on T zone-restricted BDCA3⁺ dendritic cells, again interacting with CRTAM (4). As SynCAM-1 (synaptic cell adhesion molecule-1) it binds homotypically across synaptic clefts and, like neuroligin/neurexin adhesion pairs, promotes formation of neural cell synapses (6, 7). Expression on mast cells allows attachment to neurites and fibroblasts (8, 9). As SgIGSF (spermatogenic IgSF), expression on developing sperm allows adhesion to an unknown partner on Sertoli cells that is critical for normal development (10, 11). Human IGSF4A cDNA encodes 442 amino acids including a signal peptide, three Ig-like domains (a V-type, a divergent C1-type and an I-type), a transmembrane domain and a cytoplasmic domain with protein 4.1 and PDZ domain binding sites. These sites allow connections to adaptor molecules and are critical in synaptogenesis (6, 7). The outer Ig-like domain is most likely to mediate adhesion, with altered affinity in splice variants with shorter spacing of this domain from the plasma membrane or varied glycosylation (1, 3). The five reported splice variants include a prominently expressed soluble form of about 45 kDa that inhibits adhesion (12). Human IGSF4A ECD shares 99%, 91%, 89% and 97% amino acid identity with mouse, bovine, rat and canine IGSF4A, respectively.

1. Biederer, T. et al. (2006) Genomics 87:139.
2. Kuramochi, M. et al. (2002) Nat. Genet. 27:427.
3. Boles, K. S. et al. (2005) Blood 106:779.
4. Galibert, L. et al. (2005) J. Biol. Chem. 280:21955.
5. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421.
6. Biederer, T. et al. (2002) Science 297:1525.
7. Sara, Y. et al. (2005) J. Neurosci. 25:260.
8. Furuno, T. et al. (2005) J. Immunol. 174:6934.
9. Ito, A. et al. (2003) Blood 101:2601.
10. Wakayama, T. et al. (2001) Mol. Reprod. Dev. 60:158.
11. Fujita, E. et al. (2006) Mol. Cell. Biol. 26:718.
12. Koma, Y. et al. (2004) Oncogene 23:5687.

Bioinformatics

• Gene Symbol: CADM1
• Uniprot:
  • Q9BY67()