Recombinant Human IGFBP-2 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human IGFBP-2 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.03‑0.09 µg/mL in the presence of 14 ng/mL rhIGF-II.
Source
Mouse myeloma cell line, NS0-derived human IGFBP-2 protein
Glu40-Gln328, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IGFBP2
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
36 kDa, reducing conditions
Publications
Read Publications using
674-B2 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGFBP-2 Protein, CF

  • BP2
  • IBP2
  • IBP-2
  • IGF-binding protein 2
  • IGFBP2
  • IGFBP-2
  • IGF-BP53
  • insulin-like growth factor binding protein 2 (36kD)
  • insulin-like growth factor binding protein 2, 36kDa
  • insulin-like growth factor-binding protein 2

Background

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Human IGFBP-2 cDNA encodes a 328 amino acid (aa) residue precursor protein with a putative 39 aa residue signal peptide that is processed to generate the 289 aa residue mature protein. IGFBP-2 contains an integrin receptor recognition sequence (RGD sequence) but lacks potential N-linked glycosylation sites. During development, IGFBP-2 is expressed in a number of tissues. The highest expression level is found in the central nervous system. In adults, high expression levels are also detected in the central nervous system and in a number of reproductive tissues. IGFBP-2 binds preferentially to IGF II, exhibiting a 2 - 10 fold higher affinity for IGF II than for IGF I.

  1. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3.
  2. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.

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Publications for IGFBP-2 (674-B2)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, Enzyme Assay, Western Blot.


Filter By Application
Bioassay
(4)
Enzyme Assay
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using 674-B2 Applications Species
M Kaur, AC Drake, G Hu, S Rudnick, Q Chen, R Phennicie, R Attar, J Nemeth, F Gaudet, J Chen Induction and Therapeutic Targeting of Human NPM1c+ Myeloid Leukemia in the Presence of Autologous Immune System in Mice J. Immunol., 2019;0(0):. 2019 [PMID: 30710044] (Bioassay, Human) Bioassay Human
E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs Front Endocrinol (Lausanne), 2018;9(0):36. 2018 [PMID: 29487569] (Western Blot, Human) Western Blot Human
S Bari, Q Zhong, X Fan, Z Poon, AST Lim, TH Lim, N Dighe, S Li, GNC Chiu, CLL Chai, WYK Hwang Ex Vivo Expansion of CD34CD90CD49fHematopoietic Stem and Progenitor Cells from Non-enriched Umbilical Cord Blood with Azole Compounds Stem Cells Transl Med, 2018;0(0):. 2018 [PMID: 29392885] (Bioassay, Human) Bioassay Human
TD Zaikos, MM Painter, NT Sebastian, VH Terry, KL Collins Class 1-selective histone deacetylase inhibitors enhance HIV latency reversal while preserving the activity of HDAC isoforms necessary for maximal HIV gene expression J. Virol., 2018;0(0):. 2018 [PMID: 29298886] (Bioassay, Human) Bioassay Human
Huynh H, Iizuka S, Kaba M, Kirak O, Zheng J, Lodish HF, Zhang CC Insulin-like growth factor-binding protein 2 secreted by a tumorigenic cell line supports ex vivo expansion of mouse hematopoietic stem cells. Stem Cells, 2008;26(6):1628-35. 2008 [PMID: 18369099] (Bioassay, Mouse) Bioassay Mouse
Cortesio CL, Jiang W Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006;449(1):164-70. 2006 [PMID: 16554018] (Enzyme Assay, Human) Enzyme Assay Human
Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005;333(3):1011-6. 2005 [PMID: 15964556] (Enzyme Assay, Human) Enzyme Assay Human

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Bioinformatics

Gene Symbol IGFBP2
Uniprot