Recombinant Human IFN-alpha-1a Protein, CF

Recombinant Human IFN alpha-1a Protein (Catalog # 11014-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.100-1.50 ng/mL

2 μg/lane of Recombinant Human IFN-alpha-1a Protein (Catalog # 11014-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 17‑21 kDa.

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human IFN-alpha-1a Protein, CF Summary

• Details of Functionality: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED₅₀ for this effect is 0.100-1.50 ng/mL.
• Source: Human embryonic kidney cell, HEK293-derived human IFN-alpha 1 protein
  Cys24-Glu189
• Accession #: P01562.1
• N-terminal Sequence: Cys24
• Protein/Peptide Type: Recombinant Proteins
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 17-21 kDa, under reducing conditions.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha-1a Protein, CF

• IFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alphaD;Interferon alpha-1/13;leIF D
• IFNA1
• IFNalpha 1
• IFN-alpha 1

Background

Interferons (IFN) are a family of cytokines with potent anti‑viral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). Human IFNA2 was originally cloned in the early ‘80s and now more than a dozen closely related IFN alpha subtypes have been identified in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2-4). Structurally, type I IFNs belong to the class of five helical‑bundle cytokines, with the IFNA subtypes containing 2 conserved disulfide bonds (5). Mature human IFNA1, also known as IFNA13, shares 63% amino acid (aa) sequence identity with mouse IFNA1. Two variants of human IFNA1 are known to exist, IFNA1a and IFNA1b, which only differ by a single aa at residue 137 (6). The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (7, 8). IFNA1 is the most expressed IFNA subtype and primarily expressed by plasmacytoid dendritic cells (pDC), which are the earliest cells recruited to the sites of virus entry (9). Individual IFNA subtypes are known to display unique efficacies to viral protection, and IFNA1 exhibits low potency, determined by both anti‑viral and antiproliferative activities (10). Human IFNA1 was the first IFNA to be purified and has been tested as a treatment for various diseases (11-13).

1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
2. Goeddel, D.V. et al. (1980) Nature 287:411.
3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
5. Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
6. Hussain, M. et al. (2000) J Interferon Cytokine Res. 20:763.
7. van Pesch, V. et al. (2004) J. Virol. 78:8219.
8. James, C.M. et al. (2007) Vaccine. 25(10):1856.
9. Szubin, R. et al. (2008) J Interferon Cytokine Res. 28:749.
10. Moll, H.P. et al. (2011) Cytokine. 53:52.
11. Rubinstein, M. et al. (1978) Science. 202:1289.
12. Harper, M.S. et al. (2015) PLOS Pathogens 11:e1005254.
13. George, J. and Mattapallil, J.J. (2018) Front Immunol. 9:299.

Bioinformatics

• Uniprot:
  • P01562.1()