Recombinant Human IFN-alpha 1 (Alpha D [Ala 114]) Protein

• Reactivity: Hu
• Applications: Bioactivity

Recombinant Human IFN-alpha 1 (Alpha D [Ala 114]) Protein Summary

• Additional Information: A New rh IFN-alpha 1 is Available! It has lower endotoxin specifications, and is made in-house.
• Details of Functionality:

  Interferon was titrated with the use of the cytopathic effect inhibition assay as listed:

  Bovine (MDBK/VSV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC₅₀ for interferon in this assay is ~5 U/ml. Lot Activity was derived from multiple determinations in the above assay.

  Human (A549/EMCV) – performed as described [Budd, et al. (1985) Canc. Chem. Pharm. 12:39]. The EC₅₀ for interferon in this assay is ~1 U/ml.

• Source: E. coli-derived human IFN-alpha 1 protein
• Accession #: J00210
• Protein/Peptide Type: Recombinant Proteins
• Gene: IFNA1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Endotoxin Note: <1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 19.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
• Buffer: Phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA)
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IFN-alpha 1 (Alpha D [Ala 114]) Protein

• IFL;IFN;IFNA@;IFNA13;IFN-ALPHA;IFN-alphaD;Interferon alpha-1/13;leIF D
• IFNA1
• IFNalpha 1
• IFN-alpha 1

Background

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Publications for IFN-alpha 1 (11175-1)(1)

Publication using 11175-1

• Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012-07-26;487(7408):505-9. 2012-07-26 [PMID: 22763448] (Bioassay, Human)

Bioinformatics

• Gene Symbol: IFNA1
• Uniprot:
  • J00210()