Recombinant Human His6-USP15 Protein, CF

• Reactivity: Hu
• Applications: Bioactivity
• Format: Carrier-Free

Recombinant Human His6-USP15 Protein, CF Summary

• Details of Functionality:

  Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human USP15 concentration of 5-20 nM when using Ubiquitin-AMC or Ubiquitin-Rh110 substrates (U-550, U-555).

• Source: Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP15 protein
  Met1 - Asn981 with a C-terminal 6-His tag
• Accession #: Q9Y4E8.3
• Protein/Peptide Type: Recombinant Enzymes
• Gene: USP15
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 113 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, TCEP and Glycerol.
• Purity: >85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-USP15 Protein, CF

• Deubiquitinating enzyme 15
• EC 3.1.2.15
• EC 3.4.19.12
• Gcap18
• KIAA0529
• KIAA0529ubiquitin carboxyl-terminal hydrolase 15
• MGC131982
• MGC149838
• MGC74854
• ubiquitin specific peptidase 15
• ubiquitin specific protease 15
• ubiquitin thioesterase 15
• Ubiquitin thiolesterase 15
• Ubiquitin-specific-processing protease 15
• Ubp109
• UNPH-2
• UNPH4
• USP15

Background

Ubiquitin carboxyl-terminal hydrolase 15 (USP15) is a specialized cysteine protease with a predicted molecular weight of 112 kDa.  USP15 is a member of the peptidase C19 family and the human protein shares 98% amino acid sequence identity with its mouse ortholog.  USP15 is a regulator of the TGF beta signaling pathway, where it may promote signaling strength by deubiquitination of SMAD proteins and/or the TGF beta R1.  Other reported targets of USP15 include histone H2B, NF kappa BI alpha , MDM2 and human papillomavirus type 16 E6 protein.

 

1. Eichhorn P.J., et al. (2012) Nat. Med. 18: 429.
2. Long L., et al. (2014) J. Biol. Chem. 289: 8916.
3. Voss R.M., et al. (2009) J. Virol. 83: 8885.
4. Zou Q., et al. (2014) Nat. Immunol. 15: 562.

 

Publications for USP15 (E-594)(3)

Publications using E-594

• WC Chan, X Liu, RS Magin, NM Girardi, SB Ficarro, W Hu, MI Tarazona G, CA Starnbach, A Felix, G Adelmant, AC Varca, B Hu, AS Bratt, E DaSilva, NJ Schauer, I Jaen Maiso, EK Dolen, AX Ayala, JA Marto, SJ Buhrlage Accelerating inhibitor discovery for deubiquitinating enzymes Nature Communications, 2023-02-08;14(1):686. 2023-02-08 [PMID: 36754960] (Bioassay, Human)
• E Höllmüller, S Geigges, ML Niedermeie, KM Kammer, SM Kienle, D Rösner, M Scheffner, A Marx, F Stengel Site-specific ubiquitylation acts as a regulator of linker histone H1 Nature Communications, 2021-06-09;12(1):3497. 2021-06-09 [PMID: 34108453] (Bioassay, Human)
• Teyra J, Singer A, Schmitges F, Jaynes P, Kit Leng Lui S, Polyak M, Fodil N, Krieger J, Tong J, Schwerdtfeger C, Brasher B, Ceccarelli D, Moffat J, Sicheri F, Moran M, Gros P, Eichhorn P, Lenter M, Boehmelt G, Sidhu S Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15. Structure, 2019-01-31;0(0):. 2019-01-31 [PMID: 30713027] (Bioassay, Human)

Bioinformatics

• Gene Symbol: USP15
• Uniprot:
  • Q9Y4E8.3()