Recombinant Human His6-SUMO3 Protein, CF

Product Discontinued

Recombinant Human His6-SUMO3 Protein, CF Summary

• Details of Functionality: Recombinant Human His6-SUMO3 can be conjugated to substrate proteins via the subsequent actions of an SUMO-activating (E1) enzyme, an SUMO-conjugating (E2) enzyme, and an SUMO ligase (E3). Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-SUMO3 concentration of 10-50 μM.
• Source: E. coli-derived human SUMO3 protein
  Contains a N-terminal Met-Arg-Gly-Ser and 6-His tag
• Accession #: P55854
• Protein/Peptide Type: Recombinant Proteins
• Gene: SUMO3
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl and DTT.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-SUMO3 Protein, CF

• SMT3 (suppressor of mif two 3, yeast) homolog 1
• SMT3 homolog 1
• SMT3 suppressor of mif two 3 homolog 1
• SMT3 suppressor of mif two 3 homolog 3 (S. cerevisiae)
• SMT3 suppressor of mif two 3 homolog 3 (yeast)
• SMT3A
• SMT3B
• SMT3H1
• SMT3H1small ubiquitin-related modifier 3
• SUMO-2
• SUMO3
• SUMO-3
• ubiquitin-like protein SMT3A
• Ubiquitin-like protein SMT3B

Background

Human Small Ubiquitin-like Modifier 3 (SUMO3), also known as SMT3A, is synthesized as a 103 amino acid (aa), propeptide with a predicted 11.5 kDa. SUMO3 contains a two aa C-terminal prosegment. Human SUMO3 shares 83% sequence identity with mouse SUMO3. SUMO3 also has high aa sequence homology to SUMO2 and SUMO4, 87% and 75%, respectively. SUMO3 shares only 47% sequence identity with SUMO1. SUMOs are a family of small, related proteins that can be enzymatically attached to a target protein by a post-translational modification process termed SUMOylation (1-3). All SUMO proteins share a conserved Ubiquitin domain and a C-terminal diglycine cleavage/attachment site. Following prosegment cleavage, the C-terminal glycine residue of SUMO3 is enzymatically attached to a lysine residue on a target protein. In humans, SUMO3 is conjugated to a variety of molecules in the presence of the SAE1/UBA2 SUMO-activating (E1) enzyme and the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (4,5). In yeast, the SUMO-activating (E1) enzyme is Aos1/Uba2p (6). Because of the high level of sequence homology most studies report effects of SUMO2/3. For example, addition of SUMO2/3 was shown to modulate the function of ARHGAP21, a RhoGAP protein known to be involved in cell migration (7). Other reports indicate that the conjugation by SUMO2/3, but not SUMO1, may represent an important mechanism to protect neurons during episodes of cerebral ischemia (8,9). However, studies suggest that SUMO2/3 expression is regulated in an isoform-specific manner since oxidative stress downregulated the transcription of SUMO3 but not SUMO2 (10).

SUMOylation can occur without the requirement of a specific E3 ligase activity, where SUMO is transferred directly from Ubc9 to specific substrates. SUMOylated substrates are primarily localized to the nucleus (RanGAP-1,RANBP2, PML, p53, Sp100, HIPK2) but there are also cytosolic substrates (I kappa B alpha , GLUT1,GLUT4). SUMO modification has been implicated in functions such as nuclear transport, chromosome segregation, transcriptional regulation, apoptosis, and protein stability.

1. Desterro, J.M. et al. (1997) FEBs. Lett. 417:297.
2. Bettermann, K. et al. (2012) Cancer Lett. 316:113.
3. Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
4. Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
5. Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
6. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
7. Bigarella, C.L. et al. (2012) FEBS Lett. 586:3522.
8. Datwyler, A.L. et al. (2012) J. Cereb. Blood Flow Metab. 31:2152.
9. Wang, Z. et al. (2012) Protein Expr. Purif. 82:174.
10. Sang, J. et al. (2012) Biochem. J. 435:489.

Bioinformatics

• Gene Symbol: SUMO3
• Uniprot:
  • P55854()