Recombinant Human His6-SENP1 Catalytic Domain Protein, CF

• Reactivity: Hu
• Applications: Enzyme Activity
• Format: Carrier-Free

Recombinant Human His6-SENP1 Catalytic Domain Protein, CF Summary

• Details of Functionality: Recombinant Human His6-SENP1 Catalytic Domain is a SUMO-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-SENP1 Catalytic Domain concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.
• Source: E. coli-derived human SENP1 protein
  Asp415 - Leu644 with a N-terminal 6-His tag
• Accession #: Q9P0U3.2
• Protein/Peptide Type: Recombinant Enzymes
• Gene: SENP1
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human His6-SENP1 Catalytic Domain Protein, CF

• EC 3.4.22
• EC 3.4.22.-
• SENP1
• Sentrin/SUMO-specific protease SENP1
• sentrin-specific protease 1
• SUMO1/sentrin specific peptidase 1
• SUMO1/sentrin specific protease 1
• SuPr-2

Background

SUMO/Sentrin Specific Peptidase 1 (SENP1) is a member of the SENP family of proteases (1). SENPs are a group of cysteine-type peptidases that catalyze two essential functions in the SUMO pathways: processing of full-length small Ubiquitin-related modifiers (SUMOs) to their mature forms and deconjugation of SUMOs from SUMOylated proteins. SENP1 is 644 amino acids (aa) in length with a predicted molecular weight of 73.5 kDa. Human SENP1 shares 89% aa sequence identity with the mouse and rat orthologs (1). Mammalian SENPs share a conserved C-terminal catalytic domain while the N-terminal domains have no significant similarity (2). SENP1 has broad specificity for the three mammalian SUMOs (3). It is highly expressed in testis, with lower levels being seen in thymus, pancreas, spleen, liver, ovary, and small intestine (3). SENP1 shuttles between the nucleus and cytoplasm, and it is thought that its localization is vital for the regulation for SUMOylation status of target proteins (2,4). SENP1 has been shown to enhance Androgen Receptor (AR)-dependent transcription via deSUMOylation of HDAC1, a negative regulator of AR (5,6). Consequently, SENP1 is thought to play a role in the development, progression, and metastasis of prostate cancer (6-8). SENP1 has also been shown to regulate Erythropoietin production during hypoxia by maintaining the stability of HIF1 alpha  (6). 

1. Gong, L. et al. (2000) J. Biol. Chem. 275:3355.
2. Bailey, D. & P. O'Hare (2004) J. Biol. Chem. 279:692.
3. Xu, Z. & S.W. Au (2005) Biochem. J. 386:325.
4. Kim, Y.H. et al. (2005) FEBS Lett. 579:6272.
5. Cheng, J. et al. (2004) Mol. Cell. Biol. 24:6021.
6. Zuo, Y. & J.K. Cheng (2009) Asian J. Androl. 11:36.
7. Cheng, J. et al. (2006) Neoplasia 8:667.
8. Wang, Q. et al. (2013) Oncogene 32:2493.

Publications for SENP1 (E-700)(2)

Publications using E-700

• M Brand, EB Bommeli, M Rütimann, U Lindenmann, R Riedl Discovery of a Dual SENP1 and SENP2 Inhibitor International Journal of Molecular Sciences, 2022-10-11;23(20):. 2022-10-11 [PMID: 36292935] (Bioassay)
• Liang , Qin, Dexheimer , Thomas S, Zhang , Ping, Rosenthal , Andrew S, Villamil , Mark A, You , Changjun, Zhang , Qiuting, Chen , Junjun, Ott , Christin, Sun , Hongmao, Luci , Diane K, Yuan , Bifeng, Simeonov , Anton, Jadhav , Ajit, Xiao , Hui, Wang , Yinsheng, Maloney , David J, Zhuang , Zhihao A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol, 2014-02-16;10(4):298-304. 2014-02-16 [PMID: 24531842] (Bioassay)

Bioinformatics

• Gene Symbol: SENP1
• Uniprot:
  • Q9P0U3.2()