Recombinant Human Glypican 2 Protein, CF

• Reactivity: Hu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Human Glypican 2 Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Glypican 2 at 4 µg/mL (100 µL/well) can bind Recombinant Human Midkine (Catalog # 258-MD) with a linear range of 4-200 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived human Glypican 2 protein
  Pro18-Ser553 & Ala359-Ser553, both with a C-terminal 6-His tag & Pro18-Ala359
• Accession #: Q8N158
• N-terminal Sequence: Pro18 & Ala359
• Protein/Peptide Type: Recombinant Proteins
• Gene: GPC2
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 59.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50 - 60 kDa, non-reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Glypican 2 Protein, CF

• 2410016G05Rik
• Cerebroglycan
• Glypican 2
• GPC2

Background

The glypicans (GPC) constitute a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. Based on the degree of their amino acid sequence similarity, two subfamilies of glypicans have been defined. One subfamily (sharing from 35-63% sequence homology) includes GPC1, 2, 4, and 6, while the second subfamily (sharing 54% sequence identity) includes GPC3 and 5. Proteins between the two subfamilies also share 17-25% sequence similarity (1-4). Glypicans are widely expressed in adult and fetal tissues. During embryonic development, the expression level of the various glypicans changes in a stage and tissue specific manner. GPC2, also known as cerebroglycan, is primarily expressed in developing neuronal tissues including the brain, spinal cord, dorsal root ganglia, and cranial nerves. It is found on the tracts of actively growing axons (5). Cell surface GPC2 binds midkine, indicating midkine-GPC2 interaction may participate in neuronal cell migration and neurite outgrowth (6).

1. Filmus, J and S.B. Selleck (2001) J. Clin. Invest. 108:497.
2. De Cat, B and G. David (2001) Semin. Cell Dev. Biol. 12:117.
3. Song, H. and J. Filmus (2002) Biochem. Biophys. Acta 1573:241.
4. Veugelers, M. et al. (1999) J. Biol. Chem. 274:26968.
5. Ivins, J.K. et al. (1997) Dev. Biol. 184:320.
6. Kurosawa, N. et al. (2001) Glycoconj. J. 18:499.

Publications for Glypican 2 (2304-GP)(5)

Publications using 2304-GP

• Pascual-Pasto, G;McIntyre, B;Hines, MG;Giudice, AM;Garcia-Gerique, L;Hoffmann, J;Mishra, P;Matlaga, S;Lombardi, S;Shraim, R;Schürch, PM;Yarmarkovich, M;Hofmann, TJ;Alikarami, F;Martinez, D;Tsang, M;Gil-de-Gómez, L;Spear, TT;Bernt, KM;Wolpaw, AJ;Dimitrov, DS;Li, W;Bosse, KR; CAR T-cell-mediated delivery of bispecific innate immune cell engagers for neuroblastoma Nature communications 2024-08-21 [PMID: 39164224] (Flow Cytometry, Human)
• Balke-Want, H;Keerthi, V;Gkitsas, N;Mancini, AG;Kurgan, GL;Fowler, C;Xu, P;Liu, X;Asano, K;Patel, S;Fisher, CJ;Brown, AK;Tunuguntla, RH;Patel, S;Sotillo, E;Mackall, CL;Feldman, SA; Homology-independent targeted insertion (HITI) enables guided CAR knock-in and efficient clinical scale CAR-T cell manufacturing Molecular cancer 2023-06-26 [PMID: 37365642] (Flow Cytometry, Human)
• N Li, A Quan, D Li, J Pan, H Ren, G Hoeltzel, N de Val, D Ashworth, W Ni, J Zhou, S Mackay, SM Hewitt, R Cachau, M Ho The IgG4 hinge with CD28 transmembrane domain improves VHH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer Nature Communications, 2023-04-08;14(1):1986. 2023-04-08 [PMID: 37031249] (ELISA Capture, Human)
• C Brégère, U Fisch, FS Halbeisen, C Schneider, T Dittmar, S Stricker, S Aghlmandi, R Guzman Doublecortin and Glypican-2 concentrations in the cerebrospinal fluid from infants are developmentally downregulated PLoS ONE, 2023-02-17;18(2):e0279343. 2023-02-17 [PMID: 36800341] (ELISA Control, Human)
• S Lugert, T Kremer, R Jagasia, A Herrmann, S Aigner, C Giachino, I Mendez-Dav, AM Gardier, JP Carralot, H Meisterman, A Augustin, MD Saxe, J Lamerz, G Duran-Pach, A Ducret, V Taylor, DJ David, C Czech Glypican-2 levels in cerebrospinal fluid predict the status of adult hippocampal neurogenesis Sci Rep, 2017-04-25;7(0):46543. 2017-04-25 [PMID: 28440309] (Bioassay, ELISA (Control), Human)

Bioinformatics

• Gene Symbol: GPC2
• Uniprot:
  • Q8N158()