Recombinant Human G-CSFR/CD114 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human G-CSFR/CD114 Protein Summary

Details of Functionality
Measured by its ability to inhibit the G-CSF-induced proliferation of NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1-4 µg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF.
Source
Mouse myeloma cell line, NS0-derived human G-CSF R/CD114 protein
Glu25-Pro621
Accession #
N-terminal Sequence
Glu25
Protein/Peptide Type
Recombinant Proteins
Gene
CSF3R
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
66.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
95-105 kDa, reducing conditions
Publications
Read Publication using 381-GR.

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human G-CSFR/CD114 Protein

  • CD114 antigen
  • CD114
  • colony stimulating factor 3 receptor (granulocyte)
  • CSF3R
  • Csfgr
  • G-CSF R
  • G-CSF receptor
  • GCSFR
  • G-CSFR
  • GCSFRG-CSF-R
  • granulocyte colony-stimulating factor receptor

Background

Granulocyte Colony Stimulating Factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Cell activation by G-CSF is mediated by a type I membrane protein belonging to the cytokine receptor superfamily. Human G-CSF R is 836 amino acids (aa) in length, with 24 amino acid signal sequence, a 603 aa extracellular domain, a 23 aa transmembrane domain, and a 186 aa cytoplasmic domain. As a result of alternative splicing, at least four isoforms of G-CSF R that differ in their C-terminal region exist. Isoform 2 lacks the transmembrane region and may represent a soluble form of the receptor. The existence of 80 & 85 kDa forms of soluble G-CSF R in human serum has been reported (2). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia. These mutations typically led to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophil precursors in the bone marrow and neutropenia in peripheral blood (3). Human and mouse G-CSF R have a homology of 62.5%.

G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta. Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases. Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3, STAT3, and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (1).

  1. Nicola, N.A., in Cytokine Reference, (2001) Oppenhiem, J.J. and M. Feldmann, eds. Academic Press p. 193
  2. Iwasaki, H. et al. (1999) J. Immunol. 163:6907.
  3. Mitsui, T. et al. (2003) Blood. 101:2990.

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Publications for G-CSFR/CD114 (381-GR)(1)

We have publications tested in 1 application: Bioassay.


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Bioinformatics

Gene Symbol CSF3R
Uniprot